OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

EXOC1 (NM_178237) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC218467
EXOC1 (Myc-DDK-tagged)-Human exocyst complex component 1 (EXOC1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $805.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "EXOC1 or NM_178237"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EXOC1
Synonyms BM-102; SEC3; SEC3L1; SEC3P
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218467 representing NM_178237
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGCAATCAAGCATGCATTACAAAGAGACATTTTTACACCAAATGATGAACGCCTGCTGAGCATTG
TGAATGTCTGCAAAGCAGGAAAAAAGAAAAAGAACTGTTTTTTATGTGCCACAGTGACAACTGAACGCCC
TGTGCAGGTTAAGGTGGTCAAAGTCAAGAAATCCGATAAGGGAGATTTCTACAAAAGGCAGATTGCATGG
GCCCTTCGAGATCTTGCTGTGGTAGATGCCAAAGATGCTATCAAAGAAAATCCTGAATTTGATTTACACT
TTGAAAAAATATATAAATGGGTTGCCAGCAGCACTGCTGAAAAGAATGCATTTATTTCATGCATTTGGAA
ATTGAATCAGCGATATCTCCGGAAGAAAATTGATTTTGTCAATGTTAGCTCACAGCTTTTGGAAGAATCT
GTTCCAAGTGGAGAAAATCAGAGTGTGACAGGAGGTGATGAAGAAGTAGTAGATGAATACCAAGAGTTAA
ATGCAAGAGAAGAACAGGATATCGAAATAATGATGGAAGGCTGTGAATATGCAATCTCGAATGCGGAAGC
CTTTGCAGAAAAATTGTCCAGAGAGCTGCAGGTGCTAGATGGGGCTAACATCCAGTCAATCATGGCATCT
GAAAAACAAGTCAACATCCTGATGAAATTGCTAGATGAGGCTCTAAAGGAGGTAGATCAGATTGAATTGA
AACTGAGCAGTTATGAGGAAATGCTCCAAAGTGTAAAAGAACAAATGGATCAGATCTCTGAAAGCAACCA
CCTAATTCATCTTAGTAACACTAATAATGTAAAACTCCTATCTGAGATAGAGTTCCTTGTGAACCACATG
GACTTGGCCAAAGGTCATATAAAGGCCCTTCAGGAAGGAGATCTTGCTTCTTCCAGAGGCATTGAGGCCT
GCACCAATGCTGCTGATGCCCTTCTGCAGTGCATGAATGTAGCTCTTCGACCAGGCCATGACTTGCTTCT
GGCAGTCAAACAGCAACAGCAGCGATTCAGTGATTTGCGAGAGCTTTTTGCCCGGAGACTGGCCAGTCAC
CTCAACAATGTTTTTGTTCAACAGGGTCATGATCAGAGTTCGACTCTTGCCCAACACTCTGTTGAACTGA
CTTTACCCAATCATCATCCATTTCATAGAGATTTGCTCCGATATGCCAAGCTGATGGAGTGGCTAAAGAG
TACAGATTATGGAAAATATGAAGGACTAACAAAGAATTACATGGATTATTTATCCCGACTATATGAAAGA
GAAATCAAAGATTTCTTTGAAGTTGCAAAGATCAAGATGACTGGCACAACTAAAGAAAGCAAGAAGTTTG
GTCTTCATGGAAGTTCGGGGAAATTAACTGGATCTACTTCTAGTCTAAATAAGCTCAGTGTTCAGAGTTC
AGGGAATCGCAGATCTCAGTCATCTTCCCTGTTGGATATGGGAAACATGTCTGCCTCTGATCTCGATGTT
GCTGACAGGACCAAATTTGATAAGATCTTTGAACAGGTACTAAGTGAACTGGAGCCCCTATGTCTGGCAG
AACAGGACTTCATAAGTAAATTTTTCAAACTACAGCAACATCAAAGTATGCCTGGAACTATGGCTGAAGC
AGAGGACCTGGATGGAGGAACATTATCACGGCAACATAATTGTGGCACACCACTGCCTGTTTCATCTGAG
AAAGATATGATCCGCCAAATGATGATTAAAATATTTCGCTGCATTGAGCCAGAGCTGAACAACCTAATTG
CATTAGGAGACAAAATTGATAGCTTTAACTCTCTTTATATGTTAGTCAAAATGAGTCATCATGTGTGGAC
TGCACAAAATGTGGACCCTGCTTCTTTCCTAAGTACTACATTGGGAAATGTTTTGGTGACTGTCAAAAGG
AACTTTGACAAATGCATTAGTAACCAAATAAGGCAAATGGAAGAAGTAAAGATCTCAAAAAAGAGTAAAG
TTGGAATTCTTCCATTTGTTGCTGAATTTGAAGAATTTGCTGGACTTGCAGAATCAATCTTCAAAAATGC
TGAGCGTCGTGGAGACCTGGATAAAGCATACACCAAACTTATCAGAGGAGTATTTGTTAATGTGGAGAAA
GTAGCAAATGAAAGCCAGAAGACCCCCAGGGATGTGGTTATGATGGAAAACTTTCACCATATTTTTGCAA
CTCTTTCTCGATTGAAAATCTCATGTCTAGAAGCAGAAAAAAAAGAAGCCAAACAAAAATACACAGATCA
CCTTCAGTCTTATGTCATTTACTCTTTAGGACAACCTCTTGAAAAACTAAATCATTTCTTTGAAGGTGTT
GAAGCTCGCGTGGCACAGGGCATAAGGGAGGAGGAAGTAAGTTACCAACTTGCATTTAACAAACAAGAAC
TTCGTAAAGTCATTAAGGAGTACCCTGGAAAGGAAGTAAAAAAAGGTCTAGATAACCTCTACAAGAAAGT
TGATAAACATTTATGTGAAGAAGAGAACTTACTTCAGGTGGTGTGGCACTCCATGCAAGATGAATTTATA
CGCCAGTATAAGCACTTTGAAGGTTTGATAGCTCGCTGTTATCCTGGATCTGGTGTTACAATGGAATTCA
CTATTCAGGACATTCTGGATTATTGTTCCAGCATTGCACAGTCCCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC218467 representing NM_178237
Red=Cloning site Green=Tags(s)

MTAIKHALQRDIFTPNDERLLSIVNVCKAGKKKKNCFLCATVTTERPVQVKVVKVKKSDKGDFYKRQIAW
ALRDLAVVDAKDAIKENPEFDLHFEKIYKWVASSTAEKNAFISCIWKLNQRYLRKKIDFVNVSSQLLEES
VPSGENQSVTGGDEEVVDEYQELNAREEQDIEIMMEGCEYAISNAEAFAEKLSRELQVLDGANIQSIMAS
EKQVNILMKLLDEALKEVDQIELKLSSYEEMLQSVKEQMDQISESNHLIHLSNTNNVKLLSEIEFLVNHM
DLAKGHIKALQEGDLASSRGIEACTNAADALLQCMNVALRPGHDLLLAVKQQQQRFSDLRELFARRLASH
LNNVFVQQGHDQSSTLAQHSVELTLPNHHPFHRDLLRYAKLMEWLKSTDYGKYEGLTKNYMDYLSRLYER
EIKDFFEVAKIKMTGTTKESKKFGLHGSSGKLTGSTSSLNKLSVQSSGNRRSQSSSLLDMGNMSASDLDV
ADRTKFDKIFEQVLSELEPLCLAEQDFISKFFKLQQHQSMPGTMAEAEDLDGGTLSRQHNCGTPLPVSSE
KDMIRQMMIKIFRCIEPELNNLIALGDKIDSFNSLYMLVKMSHHVWTAQNVDPASFLSTTLGNVLVTVKR
NFDKCISNQIRQMEEVKISKKSKVGILPFVAEFEEFAGLAESIFKNAERRGDLDKAYTKLIRGVFVNVEK
VANESQKTPRDVVMMENFHHIFATLSRLKISCLEAEKKEAKQKYTDHLQSYVIYSLGQPLEKLNHFFEGV
EARVAQGIREEEVSYQLAFNKQELRKVIKEYPGKEVKKGLDNLYKKVDKHLCEEENLLQVVWHSMQDEFI
RQYKHFEGLIARCYPGSGVTMEFTIQDILDYCSSIAQSH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178237
ORF Size 2637 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178237.2, NP_839955.1
RefSeq Size 3381 bp
RefSeq ORF 2640 bp
Locus ID 55763
UniProt ID Q9NV70, A0A024RDA1
Cytogenetics 4q12
MW 100.1 kDa
Summary The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of the exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. Alternatively spliced transcript variants encoding distinct isoforms have been described. provided by RefSeq, Jul 2008
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_178237" in other vectors (4)
SKU Description Size Price
RC218467L3 Lenti ORF clone of Human exocyst complex component 1 (EXOC1), transcript variant 2, Myc-DDK-tagged 10 ug
$1,105.00
RC218467L4 Lenti ORF clone of Human exocyst complex component 1 (EXOC1), transcript variant 2, mGFP tagged 10 ug
$1,105.00
RG218467 EXOC1 (tGFP-tagged) - Human exocyst complex component 1 (EXOC1), transcript variant 2 10 ug
$1,005.00
SC314858 EXOC1 (untagged)-Human exocyst complex component 1 (EXOC1), transcript variant 2 10 ug
$886.00

Plasmid Map for RC218467

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC218467
Circular map for RC218467

file-alt Citations

SmartBac, a new baculovirus system for large protein complex production
Zhai, Zhang, Yu et al
Journal of Structural Biology: X (2019)
Snippet: All human exocyst genes were purchased from Origene, USA. For each gene, two cycles of PCR were done to produce the target overlapped ends (M0492, NEB). 4V2G was digested by NdeI, KpnI and EcoRI (NEB)
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products