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ING3 (NM_019071) Human Tagged ORF Clone

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SKU
RC218424
ING3 (Myc-DDK-tagged)-Human inhibitor of growth family, member 3 (ING3), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "ING3 or NM_019071"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ING3
Synonyms Eaf4; ING2; MEAF4; p47ING3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218424 representing NM_019071
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGTACCTAGAAGACTATCTGGAAATGATTGAGCAGCTTCCTATGGATCTGCGGGACCGCTTCACGG
AAATGCGCGAGATGGACCTGCAGGTGCAGAATGCAATGGATCAACTAGAACAAAGAGTCAGTGAATTCTT
TATGAATGCAAAGAAAAATAAACCTGAGTGGAGGGAAGAGCAAATGGCATCCATCAAAAAAGACTACTAT
AAAGCTTTGGAAGATGCAGATGAGAAGGTTCAGTTGGCAAACCAGATATATGACTTGGTAGATCGACACT
TGAGAAAGCTGGATCAGGAACTGGCTAAGTTTAAAATGGAGCTGGAAGCTGATAATGCTGGAATTACAGA
AATATTAGAGAGGCGATCTTTGGAATTAGACACTCCTTCACAGCCAGTGAACAATCACCATGCTCATTCA
CATACTCCAGTGGAAAAAAGGAAATATAATCCAACTTCTCACCATACGACAACAGATCATATTCCTGAAA
AGAAATTTAAATCTGAAGCTCTTCTATCCACCCTTACGTCAGATGCCTCTAAGGAAAATACACTAGGTTG
TCGAAATAATAATTCCACAGCCTCTTCTAACAATGCCTACAATGTGAATTCCTCCCAACCTCTGGGATCC
TATAACATTGGCTCGTTATCTTCAGGAACTGGTGCAGGGGCAATTACCATGGCAGCTGCTCAAGCAGTTC
AGGCTACAGCTCAGATGAAGGAGGGACGAAGAACATCAAGTTTAAAAGCCAGTTATGAAGCATTTAAGAA
TAATGACTTTCAGTTGGGAAAAGAATTTTCAATGGCCAGGGAAACAGTTGGCTATTCATCATCTTCGGCA
CTTATGACAACATTAACACAGAATGCCAGTTCATCAGCAGCCGACTCACGGAGTGGTCGAAAGAGCAAAA
ACAACAACAAGTCTTCAAGCCAGCAGTCATCATCTTCCTCCTCCTCTTCTTCCTTATCATCGTGTTCTTC
ATCATCAACTGTTGTACAAGAAATCTCTCAACAAACAACTGTAGTGCCAGAATCTGATTCAAATAGTCAG
GTTGATTGGACTTACGACCCAAATGAACCTCGATACTGCATTTGTAATCAGGTATCTTATGGTGAGATGG
TGGGATGTGATAACCAAGATTGCCCTATAGAATGGTTCCATTATGGCTGCGTTGGATTGACAGAGGCACC
AAAAGGCAAATGGTACTGTCCACAGTGCACTGCTGCAATGAAGAGAAGAGGCAGCAGACACAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC218424 representing NM_019071
Red=Cloning site Green=Tags(s)

MLYLEDYLEMIEQLPMDLRDRFTEMREMDLQVQNAMDQLEQRVSEFFMNAKKNKPEWREEQMASIKKDYY
KALEDADEKVQLANQIYDLVDRHLRKLDQELAKFKMELEADNAGITEILERRSLELDTPSQPVNNHHAHS
HTPVEKRKYNPTSHHTTTDHIPEKKFKSEALLSTLTSDASKENTLGCRNNNSTASSNNAYNVNSSQPLGS
YNIGSLSSGTGAGAITMAAAQAVQATAQMKEGRRTSSLKASYEAFKNNDFQLGKEFSMARETVGYSSSSA
LMTTLTQNASSSAADSRSGRKSKNNNKSSSQQSSSSSSSSSLSSCSSSSTVVQEISQQTTVVPESDSNSQ
VDWTYDPNEPRYCICNQVSYGEMVGCDNQDCPIEWFHYGCVGLTEAPKGKWYCPQCTAAMKRRGSRHK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019071
ORF Size 1254 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019071.3
RefSeq Size 2221 bp
RefSeq ORF 1257 bp
Locus ID 54556
UniProt ID Q9NXR8
Cytogenetics 7q31.31
Domains PHD
Protein Families Druggable Genome, Transcription Factors
MW 46.6 kDa
Summary The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed. provided by RefSeq, Jul 2008
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"NM_019071" in other vectors (6)
SKU Description Size Price
RC218424L1 Lenti ORF clone of Human inhibitor of growth family, member 3 (ING3), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC218424L2 Lenti ORF clone of Human inhibitor of growth family, member 3 (ING3), transcript variant 1, mGFP tagged 10 ug
$757.00
RC218424L3 Lenti ORF clone of Human inhibitor of growth family, member 3 (ING3), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC218424L4 Lenti ORF clone of Human inhibitor of growth family, member 3 (ING3), transcript variant 1, mGFP tagged 10 ug
$757.00
RG218424 ING3 (tGFP-tagged) - Human inhibitor of growth family, member 3 (ING3), transcript variant 1 10 ug
$489.00 $657.00
SC128111 ING3 (untagged)-Human inhibitor of growth family, member 3 (ING3), transcript variant 1 10 ug
$457.00

Plasmid Map for RC218424

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC218424
Circular map for RC218424

file-alt Citations

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