DMC1 (NM_007068) Human Tagged ORF Clone

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SKU
RC218311
DMC1 (Myc-DDK-tagged)-Human DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (DMC1)
  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DMC1
Synonyms dJ199H16.1; DMC1H; LIM15
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218311 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGAGGATCAAGTTGTGGCGGAAGAACCAGGATTCCAAGATGAAGAGGAATCTTTGTTTCAAGATA
TTGACCTGTTACAGAAACATGGAATTAACGTGGCTGACATTAAGAAACTGAAATCAGTAGGAATCTGTAC
CATCAAAGGTATACAGATGACAACAAGAAGAGCTCTATGCAATGTCAAAGGACTCTCAGAAGCCAAAGTA
GACAAGATTAAAGAGGCAGCGAACAAACTAATTGAACCAGGATTCTTGACTGCATTTGAGTATAGTGAAA
AGAGGAAAATGGTTTTCCATATCACCACCGGGAGCCAGGAATTTGATAAGTTACTAGGAGGTGGAATTGA
AAGTATGGCAATTACAGAAGCTTTTGGAGAATTTCGTACTGGAAAAACCCAGCTTTCTCATACCCTCTGT
GTGACAGCTCAACTTCCAGGAGCTGGTGGCTACCCAGGAGGAAAGATTATCTTCATTGATACAGAAAATA
CTTTCCGTCCAGATCGCCTTAGGGACATTGCTGATCGCTTTAATGTAGACCATGATGCAGTACTGGACAA
CGTACTTTATGCACGTGCATATACTAGTGAACATCAGATGGAGCTACTTGATTATGTAGCAGCAAAGTTC
CATGAAGAAGCTGGCATCTTCAAGCTATTGATTATCGATTCAATAATGGCACTTTTTCGAGTGGATTTCA
GTGGCCGTGGGGAGTTGGCCGAACGGCAGCAAAAATTGGCCCAGATGTTGTCACGACTCCAAAAAATCTC
AGAAGAATATAACGTGGCTGTTTTTGTGACCAATCAAATGACTGCCGATCCAGGAGCAACTATGACCTTT
CAGGCAGATCCCAAAAAACCCATTGGGGGACACATTCTGGCTCATGCTTCAACAACAAGAATAAGCTTGC
GAAAGGGAAGAGGAGAGCTCAGAATTGCCAAGATTTATGACAGTCCTGAGATGCCTGAAAATGAAGCCAC
CTTCGCAATAACTGCTGGAGGAATTGGGGATGCCAAGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC218311 protein sequence
Red=Cloning site Green=Tags(s)

MKEDQVVAEEPGFQDEEESLFQDIDLLQKHGINVADIKKLKSVGICTIKGIQMTTRRALCNVKGLSEAKV
DKIKEAANKLIEPGFLTAFEYSEKRKMVFHITTGSQEFDKLLGGGIESMAITEAFGEFRTGKTQLSHTLC
VTAQLPGAGGYPGGKIIFIDTENTFRPDRLRDIADRFNVDHDAVLDNVLYARAYTSEHQMELLDYVAAKF
HEEAGIFKLLIIDSIMALFRVDFSGRGELAERQQKLAQMLSRLQKISEEYNVAVFVTNQMTADPGATMTF
QADPKKPIGGHILAHASTTRISLRKGRGELRIAKIYDSPEMPENEATFAITAGGIGDAKE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007068
ORF Size 1020 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007068.4
RefSeq Size 2281 bp
RefSeq ORF 1023 bp
Locus ID 11144
UniProt ID Q14565
Cytogenetics 22q13.1
Protein Families Druggable Genome
MW 37.7 kDa
Summary This gene encodes a member of the superfamily of recombinases (also called DNA strand-exchange proteins). Recombinases are important for repairing double-strand DNA breaks during mitosis and meiosis. This protein, which is evolutionarily conserved, is reported to be essential for meiotic homologous recombination and may thus play an important role in generating diversity of genetic information. Alternative splicing results in multiple transcript variants. provided by RefSeq, May 2013
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Documents
Resources
"NM_007068" in other vectors (4)
SKU Description Size Price
RC218311L3 Lenti ORF clone of Human DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (DMC1), Myc-DDK-tagged 10 ug
$757.00
RC218311L4 Lenti ORF clone of Human DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (DMC1), mGFP tagged 10 ug
$757.00
RG218311 DMC1 (tGFP-tagged) - Human DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (DMC1) 10 ug
$489.00 $657.00
SC308955 DMC1 (untagged)-Human DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (DMC1) 10 ug
$503.00

Plasmid Map for RC218311

Download sequence for

Cloning scheme for RC218311

Circular map for RC218311

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