KRT37 (NM_003770) Human Tagged ORF Clone

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SKU
RC218287
KRT37 (Myc-DDK-tagged)-Human keratin 37 (KRT37)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KRT37
Synonyms HA7; K37; KRTHA7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218287 representing NM_003770
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCTCCTTCTACAGCACCTCCTCATGCCCTCTGGGTTGCACCATGGCTCCTGGAGCAAGAAATGTCT
TTGTCTCTCCTATCGATGTTGGGTGCCAGCCTGTGGCAGAGGCCAATGCTGCCTCCATGTGCCTCTTGGC
CAACGTGGCACACGCCAACAGAGTCCGTGTGGGGTCGACTCCCCTGGGCCGCCCCAGCCTCTGTCTGCCC
CCAACCAGTCACACTGCTTGTCCCTTGCCAGGGACCTGTCACATTCCCGGCAACATCGGAATCTGTGGGG
CCTACGGCAAAAACACCCTGAATGGCCATGAGAAGGAGACCATGAAGTTCCTGAATGACCGCCTGGCCAA
CTACCTGGAGAAGGTGCGCCAGCTGGAGCAGGAGAATGCAGAGCTGGAGACCACACTCCTCGAGAGGAGC
AAGTGCCACGAGTCCACCGTGTGCCCCGACTACCAGTCCTACTTCCGTACAATCGAGGAGCTCCAGCAGA
AGATCCTGTGCAGCAAGGCTGAGAATGCCAGGCTGATTGTACAAATTGACAACGCGAAGCTGGCTGCTGA
TGACTTTAGGATCAAGCTGGAGAGTGAGCGCTCCCTTCACCAGCTGGTGGAGGCGGACAAGTGCGGGACG
CAGAAGCTCCTGGATGACGCGACCCTGGCCAAGGCCGACCTGGAGGCCCAGCAGGAGTCCCTGAAGGAGG
AGCAGCTCTCCCTCAAGAGCAACCACGAGCAGGAAGTAAAGATTCTGAGGAGTCAGCTGGGGGAGAAGTT
CCGGATCGAGCTGGACATTGAGCCCACCATTGACCTGAACAGGGTGTTGGGGGAGATGCGGGCTCAGTAC
GAGGCCATGGTGGAGACCAACCACCAGGATGTGGAACAGTGGTTCCAAGCCCAGTCTGAAGGCATCAGCC
TGCAGGCCATGTCCTGCTCCGAGGAGCTGCAGTGCTGCCAGTCGGAGATCCTGGAGCTGAGATGCACGGT
GAATGCCCTGGAGGTGGAGCGCCAAGCCCAGCACACCTTGAAGGACTGTCTGCAGAACTCCCTGTGTGAA
GCGGAGGACCGCTACGGCACAGAGCTGGCCCAGATGCAGAGCCTCATTAGCAACTTGGAAGAGCAGTTGT
CTGAGATCCGGGCCGACCTGGAGCGGCAGAACCAGGAGTACCAGGTGCTGCTGGACGTGAAGGCCCGGTT
GGAGAACGAGATTGCCACATACCGGAACCTTCTGGAGAGCGAGGACTGCAAACTCCCCTGCAATCCCTGT
TCCACGCCTGCCTCCTGTACTTCTTGTCCAAGCTGTGGCCCTGTCACCGGTGGGTCTCCCTCTGGCCATG
GAGCCAGCATGGGGAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC218287 representing NM_003770
Red=Cloning site Green=Tags(s)

MTSFYSTSSCPLGCTMAPGARNVFVSPIDVGCQPVAEANAASMCLLANVAHANRVRVGSTPLGRPSLCLP
PTSHTACPLPGTCHIPGNIGICGAYGKNTLNGHEKETMKFLNDRLANYLEKVRQLEQENAELETTLLERS
KCHESTVCPDYQSYFRTIEELQQKILCSKAENARLIVQIDNAKLAADDFRIKLESERSLHQLVEADKCGT
QKLLDDATLAKADLEAQQESLKEEQLSLKSNHEQEVKILRSQLGEKFRIELDIEPTIDLNRVLGEMRAQY
EAMVETNHQDVEQWFQAQSEGISLQAMSCSEELQCCQSEILELRCTVNALEVERQAQHTLKDCLQNSLCE
AEDRYGTELAQMQSLISNLEEQLSEIRADLERQNQEYQVLLDVKARLENEIATYRNLLESEDCKLPCNPC
STPASCTSCPSCGPVTGGSPSGHGASMGR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003770
ORF Size 1347 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003770.5
RefSeq Size 1718 bp
RefSeq ORF 1350 bp
Locus ID 8688
UniProt ID O76014
Cytogenetics 17q21.2
MW 49.6 kDa
Summary The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_003770" in other vectors (4)
SKU Description Size Price
RC218287L3 Lenti ORF clone of Human keratin 37 (KRT37), Myc-DDK-tagged 10 ug
$757.00
RC218287L4 Lenti ORF clone of Human keratin 37 (KRT37), mGFP tagged 10 ug
$757.00
RG218287 KRT37 (tGFP-tagged) - Human keratin 37 (KRT37) 10 ug
$657.00
SC303375 KRT37 (untagged)-Human keratin 37 (KRT37) 10 ug
$503.00

Plasmid Map for RC218287

Download sequence for

Cloning scheme for RC218287

Circular map for RC218287

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