ABCA12 (NM_015657) Human Tagged ORF Clone
SKU
RC218140
ABCA12 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family A (ABC1), member 12 (ABCA12), transcript variant 2
-
TrueORF®
TrueORF®
Expression-ready ORF plasmid with C-terminal tag(s)
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Product Data | |
Type | Human Tagged ORF Clone |
---|---|
Target Symbol | ABCA12 |
Synonyms | ARCI4A; ARCI4B; ICR2B; LI2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RC218140 representing NM_015657
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTACCTATATAAAAATTATTACTTCAGGTGACTCCGATAATATAACGCATGTGTGGAATGAGGATG ATGGACAGACCTTATCTCCAAGCAGTCTGGCTGCACAGCTCCTAATTCTGGAAAACTTTGAAGATGCCCT CTTAAATATATCAGCAAATAGTCCTTATATTCCTTACTTGGCATGTGTGAGAAATGTGACTGACAGTTTG GCCAGAGGTTCACCAGAAAATCTAAGACTCCTGCAGTCCACAATACGATTTAAAAAATCTTTTCTTCGCA ATGGTTCCTATGAAGATTACTTTCCTCCAGTTCCTGAAGTCCTAAAATCAAAACTGTCTCAACTTCGAAA CTTGACCGAACTTCTTTGTGAATCTGAAACTTTCAGTTTGATAGAGAAGTCATGCCAGCTCTCTGATATG AGCTTTGGGAGCCTGTGTGAAGAAAGTGAGTTTGATCTGCAACTCCTCGAAGCGGCAGAGCTGGGCACCG AAATAGCAGCCAGCTTACTGTACCATGACAATGTCATATCTAAAAAAGTGAGAGATTTGCTGACTGGAGA TCCAAGCAAAATTAATTTAAATATGGATCAGTTTCTAGAACAGGCACTGCAAATGAATTACTTGGAAAAT ATCACTCAGTTAATACCGATCATAGAAGCCATGCTGCATGTCAATAACAGTGCAGATGCTTCTGAAAAGC CAGGTCAGTTACTAGAAATGTTTAAAAATGTTGAAGAGCTGAAAGAAGATTTAAGGAGAACAACAGGAAT GTCCAACAGGACTATTGACAAGTTGCTGGCCATTCCCATCCCTGATAATAGAGCTGAGATTATTTCTCAG GTGTTCTGGCTGCATTCCTGTGATACTAATATCACCACTCCCAAACTAGAAGATGCAATGAAAGAATTCT GCAACCTGTCTCTTTCAGAGAGATCCCGGCAGTCTTACCTCATCGGACTCACCCTTCTGCACTACTTAAA CATTTACAACTTCACATACAAGGTGTTTTTCCCGAGGAAAGATCAAAAGCCAGTAGAAAAGATGATGGAG CTCTTCATAAGACTAAAAGAGATTCTCAATCAGATGGCTTCTGGCACACATCCGCTGCTAGACAAAATGA GATCCCTGAAGCAAATGCATCTGCCCAGAAGTGTTCCATTAACACAGGCAATGTACAGAAGCAACCGAAT GAACACACCACAAGGATCATTTAGCACCATCTCCCAAGCATTATGTTCTCAAGGAATTACCACTGAATAT TTAACTGCCATGCTGCCCTCTTCCCAGAGGCCAAAAGGCAACCACACCAAGGATTTTTTGACTTATAAAT TAACTAAAGAGCAAATTGCTTCAAAATATGGAATTCCCATAAATTCCACACCATTTTGCTTCTCCCTTTA TAAAGACATCATTAACATGCCCGCTGGACCTGTGATTTGGGCTTTCTTGAAACCTATGTTGTTGGGAAGA ATTTTGTATGCACCATATAACCCAGTCACAAAGGCAATAATGGAAAAGTCCAATGTAACTCTGAGACAGC TGGCGGAATTAAGAGAAAAATCTCAAGAGTGGATGGATAAGTCGCCACTTTTCATGAATTCCTTCCATCT GTTAAACCAGGCAATTCCAATGCTCCAGAATACTCTAAGGAACCCTTTTGTGCAAGTTTTTGTAAAGTTC TCCGTGGGACTCGATGCTGTTGAACTATTGAAACAGATAGATGAACTCGATATTCTAAGACTGAAATTAG AGAACAACATTGACATCATCGATCAGCTTAACACACTATCTTCCCTGACAGTAAATATTTCCTCTTGTGT ATTATATGACCGTATTCAGGCAGCAAAAACCATAGATGAAATGGAGAGAGAGGCTAAAAGGCTCTACAAA AGCAACGAACTCTTTGGAAGTGTTATTTTTAAGCTTCCTTCTAACAGAAGCTGGCACAGAGGCTATGACT CTGGAAATGTCTTTCTTCCTCCTGTCATAAAATATACCATCCGGATGAGTCTCAAGACCGCACAGACCAC AAGAAGCCTAAGAACCAAGATTTGGGCTCCAGGGCCACACAATTCTCCATCACACAACCAGATCTATGGC AGGGCTTTTATTTATTTACAGGATAGTATTGAAAGAGCAATCATTGAATTGCAAACTGGAAGGAACTCCC AGGAAATAGCAGTCCAGGTTCAAGCAATTCCTTATCCCTGCTTCATGAAAGACAACTTCCTAACCAGTGT CTCTTATTCTCTTCCAATTGTGCTTATGGTTGCCTGGGTTGTATTTATAGCTGCCTTTGTAAAAAAGCTT GTCTATGAGAAAGACCTCCGGCTTCATGAGTACATGAAGATGATGGGTGTGAACTCCTGCAGCCATTTCT TTGCCTGGCTTATAGAGAGTGTTGGATTTTTACTGGTTACCATCGTGATCCTCATCATTATACTCAAGTT TGGCAATATTCTTCCTAAAACAAATGGGTTCATTTTGTTCCTGTATTTTTCGGACTACAGCTTCTCGGTT ATTGCCATGAGCTATCTTATCAGTGTCTTCTTCAACAACACCAACATTGCAGCTCTGATCGGAAGCCTCA TCTACATCATTGCCTTCTTTCCATTTATTGTTCTGGTTACAGTGGAGAATGAGTTGAGCTATGTATTGAA AGTGTTCATGAGCCTGCTGTCCCCAACAGCATTCAGCTATGCAAGCCAATACATTGCACGATACGAAGAA CAGGGCATTGGTCTTCAGTGGGAAAATATGTACACCTCCCCGGTTCAGGATGACACCACCTCATTTGGCT GGCTGTGCTGTCTAATCCTAGCTGACTCTTTCATTTATTTCCTTATTGCTTGGTATGTCAGGAATGTCTT CCCAGGGACATACGGTATGGCAGCTCCCTGGTATTTTCCAATTCTTCCTTCCTATTGGAAGGAGCGATTT GGGTGTGCAGAGGTGAAGCCTGAGAAGAGCAATGGCCTCATGTTTACTAACATCATGATGCAGAACACCA ACCCATCTGCCAGTCCTGAATACATGTTTTCCTCTAACATCGAGCCTGAACCTAAAGATCTCACAGTCGG GGTTGCCCTGCATGGGGTCACAAAGATCTATGGCTCAAAAGTTGCTGTTGATAACCTCAATCTGAACTTT TATGAAGGGCATATTACTTCATTGCTGGGGCCCAATGGAGCTGGGAAAACTACTACCATTTCCATGTTAA CTGGGCTGTTTGGGGCCTCAGCAGGCACCATTTTTGTATATGGAAAAGATATCAAAACAGACCTACACAC GGTACGGAAGAACATGGGAGTCTGTATGCAGCACGACGTCTTGTTCAGTTACCTCACTACTAAGGAGCAC CTTCTCCTATATGGTTCCATCAAAGTTCCTCACTGGACTAAAAAGCAGCTCCACGAGGAAGTAAAAAGGA CTTTAAAAGATACTGGACTATATAGCCATCGTCATAAGAGAGTTGGAACACTGTCAGGAGGCATGAAGAG GAAGTTATCTATATCCATAGCTCTCATTGGTGGATCAAGGGTAGTAATTTTGGATGAACCATCTACTGGA GTTGACCCATGTTCTCGCCGAAGTATATGGGATGTTATATCCAAGAACAAAACTGCCAGAACAATCATTC TGTCAACGCACCACTTGGACGAGGCTGAAGTGCTGAGTGACCGCATCGCCTTCCTGGAGCAGGGTGGGCT TAGGTGCTGTGGGTCCCCATTTTACCTCAAGGAAGCCTTTGGCGATGGGTATCACCTCACGCTTACCAAG AAGAAGAGTCCAAATTTAAATGCAAATGCAGTATGTGACACCATGGCCGTGACAGCAATGATCCAATCAC ATCTCCCCGAAGCCTACCTCAAGGAGGATATTGGGGGAGAGCTTGTTTATGTACTTCCTCCATTCAGCAC CAAAGTCTCAGGGGCCTACCTGTCACTCCTACGGGCACTCGACAATGGCATGGGTGACCTCAACATCGGG TGCTACGGCATTTCAGATACCACCGTGGAGGAGGTCTTTCTGAACTTGACCAAAGAGTCACAAAAAAATA GTGCTATGAGTCTTGAGCACTTAACACAAAAGAAAATTGGGAATTCCAATGCCAATGGCATCTCAACTCC TGACGATTTATCTGTGAGCAGCAGCAATTTCACAGACAGAGATGACAAAATCCTGACAAGAGGAGAGAGG CTGGATGGCTTTGGACTGTTGCTGAAGAAGATCATGGCTATACTCATCAAGAGGTTCCACCACACCCGCA GGAACTGGAAAGGTCTCATTGCTCAGGTTATCCTCCCCATCGTCTTTGTTACCACTGCCATGGGCCTTGG CACACTGAGAAATTCCAGCAACAGTTATCCAGAGATTCAGATCTCCCCCTCTCTTTATGGTACCTCCGAA CAGACAGCCTTCTATGCTAATTATCACCCGAGCACGGAAGCACTTGTCTCAGCAATGTGGGACTTCCCTG GAATTGACAACATGTGTCTGAACACCAGTGATCTACAGTGTTTAAACAAAGACAGTCTGGAAAAATGGAA CACCAGTGGAGAACCCATCACTAATTTTGGTGTTTGCTCCTGCTCAGAAAATGTCCAGGAATGTCCTAAA TTTAACTATTCCCCACCGCACAGAAGAACTTACTCATCCCAGGTAATTTATAACCTCACTGGGCAACGAG TGGAAAATTATCTTATATCAACTGCAAATGAGTTTGTCCAAAAAAGATATGGAGGTTGGAGTTTTGGGCT GCCTTTGACAAAAGACCTTCGTTTTGATATAACAGGAGTCCCTGCCAATAGAACACTTGCCAAGGTATGG TATGATCCAGAAGGCTATCACTCCCTTCCAGCTTACCTCAACAGCCTGAATAATTTCCTTCTGCGAGTTA ACATGTCAAAATACGATGCTGCCCGACATGGCATCATCATGTATAGCCATCCTTATCCAGGAGTGCAAGA CCAAGAACAAGCCACAATCAGCAGTTTAATCGATATTTTAGTGGCACTGTCTATCTTGATGGGCTACTCT GTCACCACCGCCAGCTTTGTCACCTATGTTGTAAGGGAACATCAAACCAAAGCCAAACAGTTGCAGCACA TTTCAGGCATTGGCGTGACATGCTACTGGGTAACAAACTTCATTTATGACATGGTTTTCTACTTGGTGCC TGTAGCGTTTTCAATTGGTATCATTGCGATTTTCAAATTACCTGCATTCTACAGTGAAAACAACCTAGGC GCTGTATCTCTCCTACTTCTCCTGTTTGGGTATGCAACATTTTCCTGGATGTACTTGCTGGCTGGGCTCT TCCATGAAACAGGAATGGCCTTCATCACTTACGTCTGTGTCAACTTGTTTTTTGGCATTAATTCCATTGT TTCCCTGTCAGTGGTATACTTTCTTTCCAAGGAAAAGCCTAATGATCCGACTTTAGAACTTATTTCTGAA ACCCTCAAGCGCATTTTCCTGATTTTCCCACAATTCTGTTTTGGCTACGGTTTGATTGAACTTTCTCAAC AACAGTCGGTCCTAGACTTCTTAAAAGCATATGGAGTGGAATACCCAAATGAAACCTTTGAGATGAATAA ACTAGGTGCAATGTTTGTGGCTTTGGTTTCTCAGGGCACCATGTTTTTTTCCTTGCGACTCTTAATCAAC GAATCCCTGATAAAGAAACTCAGGCTTTTCTTCAGAAAATTTAATTCTTCACATGTAAGGGAGACAATAG ATGAGGATGAAGATGTGCGGGCTGAGAGATTAAGAGTTGAGAGTGGTGCAGCTGAATTTGACTTGGTCCA ACTTTATTGTCTCACAAAGACCTACCAACTTATCCACAAAAAGATTATAGCTGTAAACAACATCAGCATT GGGATACCTGCTGGAGAGTGTTTTGGGCTTCTTGGAGTGAATGGAGCAGGAAAGACCACTATATTCAAGA TGCTGACAGGAGACATCATTCCTTCAAGTGGAAACATTCTGATCAGAAATAAGACCGGATCTCTGGGTCA CGTTGATTCTCACAGCTCATTAGTTGGCTACTGTCCTCAGGAAGATGCCTTAGATGACCTGGTAACTGTG GAAGAACATTTGTATTTCTATGCCAGGGTACATGGAATTCCAGAAAAGGATATTAAAGAAACTGTTCATA AACTCCTTAGGAGACTTCACCTGATGCCCTTCAAGGACAGAGCTACCTCTATGTGCAGTTATGGCACAAA AAGAAAATTATCCACTGCACTGGCCTTGATAGGGAAACCTTCCATTCTACTGCTGGATGAGCCGAGCTCT GGCATGGATCCGAAGTCGAAACGGCACCTCTGGAAGATCATTTCAGAAGAAGTACAGAACAAATGTTCCG TCATCCTCACATCTCACAGCATGGAAGAATGTGAAGCTCTCTGTACCAGGTTGGCCATTATGGTGAATGG AAAGTTTCAATGTATTGGATCTTTGCAGCACATAAAGAGCAGGTTTGGACGAGGATTTACTGTCAAAGTT CACTTGAAGAATAACAAAGTGACCATGGAGACCCTCACAAAGTTCATGCAGCTGCACTTTCCAAAAACAT ACTTAAAAGATCAGCACCTCAGCATGCTAGAGTATCATGTACCAGTCACAGCAGGAGGAGTCGCAAACAT TTTTGATCTGCTGGAAACCAACAAGACTGCTTTAAATATTACAAATTTCTTAGTGAGTCAGACCACTCTG GAAGAGGTTTTCATCAACTTTGCCAAAGACCAGAAGTCCTATGAAACTGCTGATACCAGCAGCCAAGGTT CCACTATAAGTGTTGACTCACAAGATGACCAGATGGAGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC218140 representing NM_015657
Red=Cloning site Green=Tags(s) MFTYIKIITSGDSDNITHVWNEDDGQTLSPSSLAAQLLILENFEDALLNISANSPYIPYLACVRNVTDSL ARGSPENLRLLQSTIRFKKSFLRNGSYEDYFPPVPEVLKSKLSQLRNLTELLCESETFSLIEKSCQLSDM SFGSLCEESEFDLQLLEAAELGTEIAASLLYHDNVISKKVRDLLTGDPSKINLNMDQFLEQALQMNYLEN ITQLIPIIEAMLHVNNSADASEKPGQLLEMFKNVEELKEDLRRTTGMSNRTIDKLLAIPIPDNRAEIISQ VFWLHSCDTNITTPKLEDAMKEFCNLSLSERSRQSYLIGLTLLHYLNIYNFTYKVFFPRKDQKPVEKMME LFIRLKEILNQMASGTHPLLDKMRSLKQMHLPRSVPLTQAMYRSNRMNTPQGSFSTISQALCSQGITTEY LTAMLPSSQRPKGNHTKDFLTYKLTKEQIASKYGIPINSTPFCFSLYKDIINMPAGPVIWAFLKPMLLGR ILYAPYNPVTKAIMEKSNVTLRQLAELREKSQEWMDKSPLFMNSFHLLNQAIPMLQNTLRNPFVQVFVKF SVGLDAVELLKQIDELDILRLKLENNIDIIDQLNTLSSLTVNISSCVLYDRIQAAKTIDEMEREAKRLYK SNELFGSVIFKLPSNRSWHRGYDSGNVFLPPVIKYTIRMSLKTAQTTRSLRTKIWAPGPHNSPSHNQIYG RAFIYLQDSIERAIIELQTGRNSQEIAVQVQAIPYPCFMKDNFLTSVSYSLPIVLMVAWVVFIAAFVKKL VYEKDLRLHEYMKMMGVNSCSHFFAWLIESVGFLLVTIVILIIILKFGNILPKTNGFILFLYFSDYSFSV IAMSYLISVFFNNTNIAALIGSLIYIIAFFPFIVLVTVENELSYVLKVFMSLLSPTAFSYASQYIARYEE QGIGLQWENMYTSPVQDDTTSFGWLCCLILADSFIYFLIAWYVRNVFPGTYGMAAPWYFPILPSYWKERF GCAEVKPEKSNGLMFTNIMMQNTNPSASPEYMFSSNIEPEPKDLTVGVALHGVTKIYGSKVAVDNLNLNF YEGHITSLLGPNGAGKTTTISMLTGLFGASAGTIFVYGKDIKTDLHTVRKNMGVCMQHDVLFSYLTTKEH LLLYGSIKVPHWTKKQLHEEVKRTLKDTGLYSHRHKRVGTLSGGMKRKLSISIALIGGSRVVILDEPSTG VDPCSRRSIWDVISKNKTARTIILSTHHLDEAEVLSDRIAFLEQGGLRCCGSPFYLKEAFGDGYHLTLTK KKSPNLNANAVCDTMAVTAMIQSHLPEAYLKEDIGGELVYVLPPFSTKVSGAYLSLLRALDNGMGDLNIG CYGISDTTVEEVFLNLTKESQKNSAMSLEHLTQKKIGNSNANGISTPDDLSVSSSNFTDRDDKILTRGER LDGFGLLLKKIMAILIKRFHHTRRNWKGLIAQVILPIVFVTTAMGLGTLRNSSNSYPEIQISPSLYGTSE QTAFYANYHPSTEALVSAMWDFPGIDNMCLNTSDLQCLNKDSLEKWNTSGEPITNFGVCSCSENVQECPK FNYSPPHRRTYSSQVIYNLTGQRVENYLISTANEFVQKRYGGWSFGLPLTKDLRFDITGVPANRTLAKVW YDPEGYHSLPAYLNSLNNFLLRVNMSKYDAARHGIIMYSHPYPGVQDQEQATISSLIDILVALSILMGYS VTTASFVTYVVREHQTKAKQLQHISGIGVTCYWVTNFIYDMVFYLVPVAFSIGIIAIFKLPAFYSENNLG AVSLLLLLFGYATFSWMYLLAGLFHETGMAFITYVCVNLFFGINSIVSLSVVYFLSKEKPNDPTLELISE TLKRIFLIFPQFCFGYGLIELSQQQSVLDFLKAYGVEYPNETFEMNKLGAMFVALVSQGTMFFSLRLLIN ESLIKKLRLFFRKFNSSHVRETIDEDEDVRAERLRVESGAAEFDLVQLYCLTKTYQLIHKKIIAVNNISI GIPAGECFGLLGVNGAGKTTIFKMLTGDIIPSSGNILIRNKTGSLGHVDSHSSLVGYCPQEDALDDLVTV EEHLYFYARVHGIPEKDIKETVHKLLRRLHLMPFKDRATSMCSYGTKRKLSTALALIGKPSILLLDEPSS GMDPKSKRHLWKIISEEVQNKCSVILTSHSMEECEALCTRLAIMVNGKFQCIGSLQHIKSRFGRGFTVKV HLKNNKVTMETLTKFMQLHFPKTYLKDQHLSMLEYHVPVTAGGVANIFDLLETNKTALNITNFLVSQTTL EEVFINFAKDQKSYETADTSSQGSTISVDSQDDQMES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015657 |
ORF Size | 6831 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_015657.3, NP_056472.2 |
RefSeq Size | 8097 bp |
RefSeq ORF | 6834 bp |
Locus ID | 26154 |
UniProt ID | Q86UK0 |
Cytogenetics | 2q35 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | ABC transporters |
MW | 256.8 kDa |
Summary | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This encoded protein is a member of the ABC1 subfamily, which is the only major ABC subfamily found exclusively in multicellular eukaryotes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
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