Argonaute 2 (AGO2) (NM_012154) Human Tagged ORF Clone
- TrueORF®
SKU
RC218078
AGO2 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1
$786.00
5 Days*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Argonaute 2 |
| Synonyms | CASC7; EIF2C2; LESKRES; LINC00980; PPD; Q10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012154 |
| ORF Size | 2577 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012154.5 |
| RefSeq Size | 3567 bp |
| RefSeq ORF | 2580 bp |
| Locus ID | 27161 |
| UniProt ID | Q9UKV8 |
| Cytogenetics | 8q24.3 |
| Domains | PAZ, Piwi |
| Protein Families | Druggable Genome |
| MW | 97 kDa |
| Summary | This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012154" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC218078L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,086.00
|
|
| RC218078L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1, mGFP tagged | 10 ug |
$1,086.00
|
|
| RC218078L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,086.00
|
|
| RC218078L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1, mGFP tagged | 10 ug |
$1,086.00
|
|
| RG218078 | AGO2 (tGFP-tagged) - Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1 | 10 ug |
$789.00
$986.00
|
|
| SC111241 | AGO2 (untagged)-Human eukaryotic translation initiation factor 2C, 2 (EIF2C2), transcript variant 1 | 10 ug |
$787.00
|
|
Citations
An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells
Li, Dong, Li et al
Sci Rep (2017) 7 (1), 7153 DOI: 10.1038/s41598-017-07332-3
Li, Dong, Li et al
Sci Rep (2017) 7 (1), 7153 DOI: 10.1038/s41598-017-07332-3
AI Summary: OriGene products pCMV/AGO2 and pCMV/GADD45A were used in this study to overexpress AGO2 and GADD45A, respectively, enabling the authors to investigate the post-transcriptional regulation of GADD45A by miR-138 in non-small-cell lung cancer cells.
Snippet: used as a negative control (Promega, Madison, WI, USA), pRC/p53 containing the entire p53 coding region was obtained from Sino Biological (Sino Biological Inc, Beijing, China), and pCMV/AGO2 and pCMV/GADD45A were purchased from OriGene (OriGene Technologies ... All 4 versions Cite SaveSaving
Dysregulation of a family of short noncoding RNAs, tsRNAs, in human cancer
Pekarsky, Balatti, Palamarchuk et al
PNAS (2016)
Pekarsky, Balatti, Palamarchuk et al
PNAS (2016)
Cited by 265
AI Summary: OriGene products contributed to this paper by providing the pCMV6-Entry-mycDDK tagged TrueORF gold expression vectors for PiwiL2 (SKU: RC205464), Ago1 (SKU: RC209163), and Ago2 (SKU: RC218078), which were used to generate constructs for studying protein interactions with tsRNAs in cancer research.
Snippet: For pMAX-piwiL2, pMAX-ago1, and pMAX-ago2, the ORFs of PiwiL2, Ago1, and Ago2 were subcloned in pcDNA4/HisMax (Invitrogen) vector from pCMV6-Entry-mycDDK tagged TrueORF gold expression vector purchased from OriGene [stock-keeping unit (SKU) nos. RC205464, RC209163, andRC218078, respectively]
Showing 1-2 of 2 papers.
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