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PAN3 (NM_175854) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC218027
PAN3 (Myc-DDK-tagged)-Human PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $629.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PAN3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218027 representing NM_175854
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAGTGGCGGCGGCCTCCCGCCCCCCTCGGCCGCCGCCTCCCCTTCCTCCTCCTCGCTGGCGGCGG
CGGTGGCGGTGGTGGCCCCGCCGGGGGTCGGGGGTGTCCCCGGCGGGGCGGCGGTAGGAGTGAAGCTGAA
GTACTGCCGCTACTACGCTAAGGATAAGACTTGCTTCTACGGGGAGGAGTGTCAGTTCCTGCATGAGGAC
CCTGCCGCCGGGGCTGCCCCGGGCCTCGGCCTCCATAGCAACAGCGTCCCCCTGGCTCTGGCTGGTGCAC
CCGTGGCCGGCTTTCCGCCGGGAGCCGTCGCGGGCGGGGGAGCTGGGCCGCCCCCCGGGCCCAAGAAGCC
GGACCTGGGGGACCCGGGGACCGGAGCCGCAGCCGGCGGAGGAGGCAGTAGCGGGGGACTCGATGGACCG
CGGCTGGCAATTCCAGGAATGGATGGAGGTGCTTTAACTGATACAAGTCTCACAGATTCCTATTTTAGCA
CCAGCTTTATTGGAGTCAATGGATTTGGAAGCCCTGTAGAAACAAAATATCCCCTGATGCAGAGAATGAC
TAATAGTAGCAGCTCCCCAAGCCTTCTAAATGACAGTGCCAAGCCATATTCAGCCCATGATCCTCTAACA
TCACCTGCTTCATCCTTGTTTAATGACTTTGGTGCCCTCAACATCTCTCAGAGACGAAAGCCAAGGAAGT
ATCGCCTGGGGATGCTGGAGGAGAGGCTAGTTCCGATGGGATCAAAGGCACGAAAAGCAAAGAACCCTAT
TGGCTGCCTTGCTGACAGGTGTAAATCAGGAGTACCCATCAATATGGTTTGGTGGAACAGAGTCACAGAA
AACAATTTACAGACACCAAATCCTACTGCAAGCGAGTTTATTCCTAAAGGAGGATCAACCTCCAGGCTGA
GTAACGTGTCCCAGTCAAATATGTCTGCCTTCTCTCAAGTTTTCTCTCACCCATCCATGGGAAGCCCTGC
TACTGCTGGATTAGCGCCAGGAATGTCGTTGTCTGCTGGGTCTTCCCCTCTTCATTCCCCCAAGATTACT
CCACATACTTCTCCTGCTCCCAGAAGAAGAAGTCACACTCCAAATCCAGCAAGTTACATGGTGCCTTCTA
GTGCCTCTACATCTGTTAATAATCCTGTTTCTCAGACTCCGTCTTCTGGTCAGGTGATCCAAAAGGAAAC
TGTTGGTGGGACGACTTACTTCTATACAGACACAACTCCAGCACCTTTGACTGGAATGGTGTTTCCAAAC
TATCATATTTATCCTCCAACTGCACCTCACGTTGCTTATATGCAACCGAAAGCAAACGCACCTTCCTTCT
TCATGGCTGATGAACTCCGACAGGAGCTGATCAACAGACATTTAATAACAATGGCTCAAATTGATCAAGC
AGATATGCCAGCAGTTCCTACAGAGGTTGACAGCTACCATAGCCTATTCCCTCTAGAACCACTGCCACCT
CCCAACCGGATACAGAAATCAAGTAATTTTGGATATATTACATCTTGCTACAAAGCTGTAAACAGCAAAG
ATGATCTGCCATATTGCCTTCGGAGGATACATGGTTTTCGTCTTGTTAACACAAAGTGCATGGTGTTGGT
CGACATGTGGAAGAAAATTCAACACTCAAATATCGTAACTTTGCGTGAAGTATTTACCACTAAAGCATTT
GCTGAGCCCTCTCTTGTGTTTGCATATGATTTCCATGCTGGAGGAGAAACTATGATGAGCAGACACTTTA
ATGACCCTAATGCTGATGCCTACTTCACCAAGAGAAAGTGGGGTCAGCACGAGGGACCATTGCCCAGGCA
GCATGCTGGATTATTGCCAGAATCTCTTATTTGGGCATATATTGTCCAACTAAGTTCTGCATTGCGTACC
ATTCATACAGCAGGTTTGGCATGTCGAGTTATGGATCCAACAAAGATTCTGATAACTGGCAAAACAAGGT
TGCGAGTAAATTGTGTTGGAGTTTTTGATGTTTTAACATTTGATAACAGTCAAAATAATAATCCATTGGC
ATTAATGGCCCAGTACCAGCAAGCAGATCTGATATCATTAGGAAAAGTTGTGTTGGCTTTGGCTTGCAAC
TCTTTGGCAGGAATTCAGCGAGAGAATTTACAGAAAGCCATGGAACTGGTGACAATCAACTATTCCTCTG
ACCTGAAGAATCTGATTTTGTATTTGTTGACTGACCAAAACAGGATGCGAAGTGTAAATGACATCATGCC
CATGATTGGTGCTCGATTTTATACTCAATTGGATGCTGCTCAAATGAGAAATGATGTCATAGAGGAAGAC
CTTGCAAAGGAGGTTCAAAATGGAAGACTGTTTAGGCTCCTAGCAAAATTGGGAACAATCAATGAGAGGC
CGGAGTTTCAGAAGGATCCCACTTGGTCAGAGACTGGAGACCGTTATCTGTTGAAACTCTTTAGGGATCA
TCTTTTTCATCAGGTGACAGAAGCAGGTGCTCCCTGGATTGACCTCAGTCATATAATTTCTTGTCTTAAC
AAGCTAGATGCTGGTGTGCCAGAAAAAATAAGCCTGATTTCCAGAGATGAGAAGAGTGTACTTGTGGTGA
CCTACAGTGACTTAAAGCGCTGCTTTGAAAATACTTTTCAAGAACTGATTGCAGCTGCAAATGGTCAGTT
G

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC218027 protein sequence
Red=Cloning site Green=Tags(s)

MNSGGGLPPPSAAASPSSSSLAAAVAVVAPPGVGGVPGGAAVGVKLKYCRYYAKDKTCFYGEECQFLHED
PAAGAAPGLGLHSNSVPLALAGAPVAGFPPGAVAGGGAGPPPGPKKPDLGDPGTGAAAGGGGSSGGLDGP
RLAIPGMDGGALTDTSLTDSYFSTSFIGVNGFGSPVETKYPLMQRMTNSSSSPSLLNDSAKPYSAHDPLT
SPASSLFNDFGALNISQRRKPRKYRLGMLEERLVPMGSKARKAKNPIGCLADRCKSGVPINMVWWNRVTE
NNLQTPNPTASEFIPKGGSTSRLSNVSQSNMSAFSQVFSHPSMGSPATAGLAPGMSLSAGSSPLHSPKIT
PHTSPAPRRRSHTPNPASYMVPSSASTSVNNPVSQTPSSGQVIQKETVGGTTYFYTDTTPAPLTGMVFPN
YHIYPPTAPHVAYMQPKANAPSFFMADELRQELINRHLITMAQIDQADMPAVPTEVDSYHSLFPLEPLPP
PNRIQKSSNFGYITSCYKAVNSKDDLPYCLRRIHGFRLVNTKCMVLVDMWKKIQHSNIVTLREVFTTKAF
AEPSLVFAYDFHAGGETMMSRHFNDPNADAYFTKRKWGQHEGPLPRQHAGLLPESLIWAYIVQLSSALRT
IHTAGLACRVMDPTKILITGKTRLRVNCVGVFDVLTFDNSQNNNPLALMAQYQQADLISLGKVVLALACN
SLAGIQRENLQKAMELVTINYSSDLKNLILYLLTDQNRMRSVNDIMPMIGARFYTQLDAAQMRNDVIEED
LAKEVQNGRLFRLLAKLGTINERPEFQKDPTWSETGDRYLLKLFRDHLFHQVTEAGAPWIDLSHIISCLN
KLDAGVPEKISLISRDEKSVLVVTYSDLKRCFENTFQELIAAANGQL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_175854
ORF Size 2661 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175854.7, NP_787050.6
RefSeq Size 5643 bp
RefSeq ORF 2664 bp
Locus ID 255967
UniProt ID Q58A45
Cytogenetics 13q12.2
Protein Families Druggable Genome
MW 76 kDa
Summary Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175854" in other vectors (4)
SKU Description Size Price
RC218027L3 Lenti ORF clone of Human PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN3), Myc-DDK-tagged 10 ug
$929.00
RC218027L4 Lenti ORF clone of Human PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN3), mGFP tagged 10 ug
$929.00
RG218027 PAN3 (tGFP-tagged) - Human PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN3) 10 ug
$829.00
SC314436 PAN3 (untagged)-Human PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) (PAN3) 10 ug
$894.00

file-alt Citations

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