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EIF5AL1 (NM_001099692) Human Tagged ORF Clone

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SKU
RC217890
EIF5AL1 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 5A-like 1 (EIF5AL1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EIF5AL1
Synonyms bA342M3.3; eIF-4D; eIF-5A; eIF-5A-1; eIF-5A1; EIF5A; EIF5AP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217890 representing NM_001099692
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGATGATTTGGACTTCGAGACAGGAGATGCAGGGGCCTCAGCCACCTTCCCAATGCAGTGCTCAG
CATTACGTAAGAATGGCTTTGTGGTGCTCAAAGGCTGGCCATGTAAGATCGTGGAGATGTCTGCTTCGAA
GACTGGCAAGCACGGCCACGCCAAGGTCCATCTGGTTGGTATTGACATCTTTACTGGGAAGAAATATGAA
GATATCTGCCCGTCAACTCATAATATGGATGTCCCCAACATCAAAAGGAATGACTTCCAGCTGATTGGCA
TCCAGGATGGGTACCTATCACTGCTCCAGGACAGCGGGGAGGTACCAGAGGACCTTCGTCTCCCTGAGGG
AGACCTTGGCAAGGAGATTGAGCAGAAGTACGACTGTGGAGAAGAGATCCTGATCACGGTGCTGTCTGCC
ATGACAGAGGAGGCAGCTGTTGCAATCAAGGCCATGGCAAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC217890 representing NM_001099692
Red=Cloning site Green=Tags(s)

MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGWPCKIVEMSASKTGKHGHAKVHLVGIDIFTGKKYE
DICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQDSGEVPEDLRLPEGDLGKEIEQKYDCGEEILITVLSA
MTEEAAVAIKAMAK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001099692
ORF Size 462 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001099692.2
RefSeq Size 3849 bp
RefSeq ORF 465 bp
Locus ID 143244
UniProt ID Q6IS14
Cytogenetics 10q22.3
MW 16.6 kDa
Summary mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).UniProtKB/Swiss-Prot Function
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"NM_001099692" in other vectors (4)
SKU Description Size Price
RC217890L3 Lenti ORF clone of Human eukaryotic translation initiation factor 5A-like 1 (EIF5AL1), Myc-DDK-tagged 10 ug
$450.00
RC217890L4 Lenti ORF clone of Human eukaryotic translation initiation factor 5A-like 1 (EIF5AL1), mGFP tagged 10 ug
$450.00
RG217890 EIF5AL1 (tGFP-tagged) - Human eukaryotic translation initiation factor 5A-like 1 (EIF5AL1) 10 ug
$350.00
SC316658 EIF5AL1 (untagged)-Human eukaryotic translation initiation factor 5A-like 1 (EIF5AL1) 10 ug
$165.00

Plasmid Map for RC217890

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC217890
Circular map for RC217890

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