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SFTA2 (NM_205854) Human Tagged ORF Clone

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SKU
RC217867
SFTA2 (Myc-DDK-tagged)-Human surfactant associated 2 (SFTA2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SFTA2
Synonyms GSGL541; SFTPG; SP-G; UNQ541
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217867 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGTCTGGGCTGCCCCTTGTCCTCCTCTTGACCCTCCTTGGCAGCTCACATGGAACAGGACCGGGTA
TGACTTTGCAACTGAAGCTGAAGGAGTCTTTTCTGACAAATTCCTCCTATGAGTCCAGCTTCCTGGAATT
GCTTGAAAAGCTCTGCCTCCTCCTCCATCTCCCTTCAGGGACCAGCGTCACCCTCCACCATGCAAGATCT
CAACACCATGTTGTCTGCAACACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC217867 protein sequence
Red=Cloning site Green=Tags(s)

MGSGLPLVLLLTLLGSSHGTGPGMTLQLKLKESFLTNSSYESSFLELLEKLCLLLHLPSGTSVTLHHARS
QHHVVCNT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_205854
ORF Size 234 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_205854.3
RefSeq Size 462 bp
RefSeq ORF 237 bp
Locus ID 389376
UniProt ID Q6UW10, A0A1U9X8K0
Cytogenetics 6p21.33
Protein Families Secreted Protein
MW 8.4 kDa
Summary Putative surfactant protein.UniProtKB/Swiss-Prot Function
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"NM_205854" in other vectors (4)
SKU Description Size Price
RC217867L3 Lenti ORF clone of Human surfactant associated 2 (SFTA2), Myc-DDK-tagged 10 ug
$450.00
RC217867L4 Lenti ORF clone of Human surfactant associated 2 (SFTA2), mGFP tagged 10 ug
$450.00
RG217867 SFTA2 (tGFP-tagged) - Human surfactant associated 2 (SFTA2) 10 ug
$489.00
SC308242 SFTA2 (untagged)-Human surfactant associated 2 (SFTA2) 10 ug
$165.00

Plasmid Map for RC217867

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file-download TXT (FASTA format)

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Cloning scheme for RC217867
Circular map for RC217867

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