HNF 4 alpha (HNF4A) (NM_000457) Human Tagged ORF Clone

Snippet: …Additionally, the intestine-specific knockout of HNF4α has been shown to confer protection against inflammatory bowel dis;wn in a humidified incubator at 37 °C under an atmosphere of 5% CO2. 2.2. HNF4α Overexpression The pCMV6-Entry vector (PS100001) and human HNF4α cDNA clone (RC217863) were purchased from Origene Technologies, Inc. …

Snippet: ditions between two light onset schedules which are apart by 8 h every 3 days from 7 to 11 week of age. Plasmid constructs, lentivirus production, and generation of stable cell lines EGFP, Hnf4a2 (OriGene, #RC217863), and Hnf4a8 (OriGene, #RC238302) genes were subcloned into the lentiviral vector pLV-EF1a-IRES-Ametrine. The constructed vectors were then co-transfected with the envelope and pack

Snippet: … N/A N/A ------------------------- Recombinant DNA ------------------------- Plasmid: pGW.UAS-HNF4-3xHA.attB FlyORF N/A HNF4 alpha (NM_000457) Human Myc-tagged ORF Clone Origene Cat# RC217863 pWPXLd lentiviral vector Addgene Cat# 12258 plasmids psPax2 Addgene Cat# 12260 pMD2.G Addgene Cat# 12259 ------------------------- Software and Algorithms;... … N/A N/A ------------------------- Recombinant DNA ------------------------- Plasmid: pGW.UAS-HNF4-3xHA.attB FlyORF N/A HNF4 alpha (NM_000457) Human Myc-tagged ORF Clone Origene Cat# RC217863 pWPXLd lentiviral vector Addgene Cat# 12258 plasmids psPax2 Addgene Cat# 12260 pMD2.G Addgene Cat# 12259 ------------------------- Software and Algorithms;;Plasmid: pGW.UAS-HNF4-3xHA.attB FlyORF N/A HNF4 alpha (NM_000457) Human Myc-tagged ORF Clone Origene Cat# RC217863 pWPXLd lentiviral vector Addgene Cat# 12258 plasmids psPax2 Addgene Cat# 12260 pMD2.G Addgene Cat# 12259

Snippet: Complementary DNA of the five hepatic transcription factors were amplified from human full-length TrueClones (Origene) and inserted into pCDH-EF1-MCS-T2A-Puro (System Biosciences) according to the user’s manual. c-MYC was cloned into an inducible system of Fu-tet-hOct4 (hOct4 was replaced by cMYC).37 FUdeltaGW-rtTA was from Addgene. The oligonucleotides encodi

Snippet: … The HBA1, STMN1, HIC1, HNF4A and PSAT1 expression plasmids were purchased from Origene (Beijing, China). The HBA1-sh1, STMN1-sh1, HIC1-sh1, HNF4A-sh1 and PSAT1-sh1 were purchased from Biolink (Shanghai, China). The HBA1-sh2, STMN1-sh2, HIC1-sh2, HNF4A-sh2 and PSAT1-sh2 were purc;... … The HBA1, STMN1, HIC1, HNF4A and PSAT1 expression plasmids were purchased from Origene (Beijing, China). The HBA1-sh1, STMN1-sh1, HIC1-sh1, HNF4A-sh1 and PSAT1-sh1 were purchased from Biolink (Shanghai, China). The HBA1-sh2, STMN1-sh2, HIC1-sh2, HNF4A-sh2 and PSAT1-sh2 were purc;The HBA1, STMN1, HIC1, HNF4A and PSAT1 expression plasmids were purchased from Origene (Beijing, China). The HBA1-sh1, STMN1-sh1, HIC1-sh1, HNF4A-sh1 and PSAT1-sh1 were purchased from Biolink (Shanghai, China)

Snippet: The coding regions of Foxa2, HNF4α, and GATA4 (NM_021784.4, NM_000457.4, and NM_001308093.1) were amplified using a cDNA ORF clone (OriGene, Rockville, MD, USA) and cloned into a pEGFP-C1 vector (Clontech, Palo Alto, CA, USA) View as HTML