HNF 4 alpha (HNF4A) (NM_000457) Human Untagged Clone

SKU SC123863

HNF4A (untagged)-Human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: HNF 4 alpha
• Synonyms: FRTS4; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF-14; TCF14
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000457 edited; AATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCG; TCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGAGAGGGCA; CTGGGAGGAGGCAGTGGGAGGGCGGAGGGCGGGGGCCTTCGGGGTGGGCGCCCAGGGTAG; GGCAGGTGGCCGCGGCGTGGAGGCAGGGAGAATGCGACTCTCCAAAACCCTCGTCGACAT; GGACATGGCCGACTACAGTGCTGCACTGGACCCAGCCTACACCACCCTGGAATTTGAGAA; TGTGCAGGTGTTGACGATGGGCAATGACACGTCCCCATCAGAAGGCACCAACCTCAACGC; GCCCAACAGCCTGGGTGTCAGCGCCCTGTGTGCCATCTGCGGGGACCGGGCCACGGGCAA; ACACTACGGTGCCTCGAGCTGTGACGGCTGCAAGGGCTTCTTCCGGAGGAGCGTGCGGAA; GAACCACATGTACTCCTGCAGATTTAGCCGGCAGTGCGTGGTGGACAAAGACAAGAGGAA; CCAGTGCCGCTACTGCAGGCTCAAGAAATGCTTCCGGGCTGGCATGAAGAAGGAAGCCGT; CCAGAATGAGCGGGACCGGATCAGCACTCGAAGGTCAAGCTATGAGGACAGCAGCCTGCC; CTCCATCAATGCGCTCCTGCAGGCGGAGGTCCTGTCCCGACAGATCACCTCCCCCGTCTC; CGGGATCAACGGCGACATTCGGGCGAAGAAGATTGCCAGCATCGCAGATGTGTGTGAGTC; CATGAAGGAGCAGCTGCTGGTTCTCGTTGAGTGGGCCAAGTACATCCCAGCTTTCTGCGA; GCTCCCCCTGGACGACCAGGTGGCCCTGCTCAGAGCCCATGCTGGCGAGCACCTGCTGCT; CGGAGCCACCAAGAGATCCATGGTGTTCAAGGACGTGCTGCTCCTAGGCAATGACTACAT; TGTCCCTCGGCACTGCCCGGAGCTGGCGGAGATGAGCCGGGTGTCCATACGCATCCTTGA; CGAGCTGGTGCTGCCCTTCCAGGAGCTGCAGATCGATGACAATGAGTATGCCTACCTCAA; AGCCATCATCTTCTTTGACCCAGATGCCAAGGGGCTGAGCGATCCAGGGAAGATCAAGCG; GCTGCGTTCCCAGGTGCAGGTGAGCTTGGAGGACTACATCAACGACCGCCAGTATGACTC; GCGTGGCCGCTTTGGAGAGCTGCTGCTGCTGCTGCCCACCTTGCAGAGCATCACCTGGCA; GATGATCGAGCAGATCCAGTTCATCAAGCTCTTCGGCATGGCCAAGATTGACAACCTGTT; GCAGGAGATGCTGCTGGGAGGGTCCCCCAGCGATGCACCCCATGCCCACCACCCCCTGCA; CCCTCACCTGATGCAGGAACATATGGGAACCAACGTCATCGTTGCCAACACAATGCCCAC; TCACCTCAGCAACGGACAGATGTGTGAGTGGCCCCGACCCAGGGGACAGGCAGCCACCCC; TGAGACCCCACAGCCCTCACCGCCAGGTGGCTCAGGGTCTGAGCCCTATAAGCTCCTGCC; GGGAGCCGTCGCCACAATCGTCAAGCCCCTCTCTGCCATCCCCCAGCCGACCATCACCAA; GCAGGAAGTTATCTAGCAAGCCGCTGGGGCTTGGGGGCTCCACTGGCTCCCCCCAGCCCC; CTAAGAGAGCACCTGGTGATCACGTGGTCACGGCAAAGGAAGACGTGATGCCAGGACCAG; TCCCAGAGCAGGAATGGGAAGGATGAAGGGCCCGAGAACATGGCCTAAGGGCCACATCCC; ACTGCCACCCTTGACGCCCTGCTCTGGATAACAAGACTTTGACTTGGGGAGACCTCTACT; GCCTTGGACAACTTTTCTCATGTTGAAGCCACTGCCTTCACCTTCACCTTCATCCATGTC; CAACCCCCGACTTCATCC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000457 unedited; CGCTCATTCTGGACGGCTTCCTTCTTCATGCCAGCCCGGAAGCATTTCTTGAGCCTGCAG; TAGCGGCACTGGTTCCTCTTGTCTTTGTCCACCACGCACTGCCGGCTAAATCTGCAGGAG; TACATGTGGTTCTTCCGCACGCTCCTCCGGAAGAAGCCCTTGCAGCCGTCACAGCTCGAG; GCACCGTAGTGTTTGCCCGTGGCCCGGTCCCCGCAGATGGCACACAGGGCGCTGACACCC; AGGCTGTTGGGCGCGTTGAGGTTGGTGCCTTCTGATGGGGACGTGTCATTGCCCATCGTC; AACACCTGCACATTCTCAAATTCCAGGGTGGTGTAGGCTGGGTCCAGTGCAGCACTGTAG; TCGGCCATGTCCATGTCGACGAGGGTTTTGGAGAGTCGCATTCTCCCTGCCTCCACGCCG; CGGCCACCTGCCCTACCCTGGGCGCCCACCCCGAAGGCCCCCGCCCTCCGCCCTCCCACT; GCCTCCTCCCAGTGCCCTCTCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATT; ACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGC; CTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTCCCGTTGA; TTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGT; GAGTCANACCGCTATCCACGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000457 unedited; GGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA; TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTCC; TTAATATTTATCAGCAAACAGGATGCTCGACCCTAAGTGAATTTTAGTGCAGCCTCAGGC; CAATCTTGGTCCTGGGAGTGGGCAGGGTTCCCAGAAGAACGAGTCTGGTTTCTGAGGCTG; TAGAAGGGAGCCGGAAGCCCCTCAACTTGATCACGGTGAGAACACAGGGAGCCTTTGGAG; AAGCTATTCAGCCACTGTAGTTAAGAGCTCCTGTTCTGATCCAGGGAGACCTGGGTTCAA; GTCCTGACTCAGCCACTTCCTAGTTGTGTGAGTTTCAGAAAAAAAATCACTTCACCTCTT; AGAACGCAATTTCAGCTTCTGTAACAATCTCTAGGTTAGGAGGGAGTGGGGCGGGTGAGG; GCGGGGAGGGAGGAGGAGAATAAAAAACAAAAACAATCTCTAGGTTAATAGGGAGGAAGG; GAGGATTAAATGAGATGATGCATGTCAGATGCCTTAAGACAGTGCCTGGGAGTAAGGAAG; AGCTTGAGACAGGCCCTGGGAGCTTATTCCTCCTGGATGTCACTCTGATGTGAGGGTTTA; CCCATCTTGTCCTCTCCAGCCCCAAGCCTCATTACTCTCACCCACATTATATCATCCACC; CCACCTGACCTCCCAGCTTC;
• Restriction Sites: Please inquire
• ACCN: NM_000457
• Insert Size: 3300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000457.3, NP_000448.3
• RefSeq Size: 3239 bp
• RefSeq ORF: 1425 bp
• Locus ID: 3172
• UniProt ID: P41235
• Cytogenetics: 20q13.12
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Summary: The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]; Transcript Variant: This variant (2) encodes the longest isoform (2, also known as HNF4alpha2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.