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OR52E2 (NM_001005164) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC217838
OR52E2 (Myc-DDK-tagged)-Human olfactory receptor, family 52, subfamily E, member 2 (OR52E2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol OR52E2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217838 representing NM_001005164
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCTTCCCAATGACACCCAGTTTCACCCCTCCTCCTTCCTGTTGCTGGGGATCCCAGGACTAGAAA
CACTTCACATCTGGATCGGCTTTCCCTTCTGTGCTGTGTACATGATCGCACTCATAGGGAACTTCACTAT
TCTACTTGTGATCAAGACTGACAGCAGCCTACACCAGCCCATGTTCTACTTCCTGGCCATGTTGGCCACC
ACTGATGTGGGTCTCTCAACAGCTACCATCCCTAAGATGCTTGGAATCTTCTGGATCAACCTCAGAGGGA
TCATCTTTGAAGCCTGCCTCACCCAGATGTTTTTTATCCACAACTTCACACTTATGGAGTCAGCAGTCCT
TGTGGCAATGGCTTATGACAGCTATGTGGCCATCTGCAATCCACTCCAATATAGCGCCATCCTCACCAAC
AAGGTTGTTTCTGTGATTGGTCTTGGTGTGTTTGTGAGGGCTTTAATTTTCGTCATTCCCTCTATACTTC
TTATATTGCGGTTGCCCTTCTGTGGGAATCATGTAATTCCCCACACCTACTGTGAGCACATGGGTCTTGC
TCATCTATCTTGTGCCAGCATCAAAATCAATATTATTTATGGTTTATGTGCCATTTGTAATCTGGTGTTT
GACATCACAGTCATTGCCCTTTCTTATGTGCATATTCTTTGTGCTGTTTTCCGTCTTCCTACTCATGAAG
CCCGACTCAAGTCCCTCAGCACATGTGGTTCACATGTGTGTGTAATCCTTGCCTTCTATACACCAGCCCT
CTTTTCCTTTATGACTCATTGCTTTGGCCGAAATGTGCCCCGCTATATCCATATACTCCTAGCCAATCTC
TATGTTGTGGTGCCACCAATGCTCAATCCTGTCATATATGGAGTCAGAACCAAGCAGATCTATAAATGTG
TAAAGAAAATATTATTGCAGGAACAAGGAATGGAAAAGGAAGAGTACCTAATACATACGAGGTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC217838 representing NM_001005164
Red=Cloning site Green=Tags(s)

MFLPNDTQFHPSSFLLLGIPGLETLHIWIGFPFCAVYMIALIGNFTILLVIKTDSSLHQPMFYFLAMLAT
TDVGLSTATIPKMLGIFWINLRGIIFEACLTQMFFIHNFTLMESAVLVAMAYDSYVAICNPLQYSAILTN
KVVSVIGLGVFVRALIFVIPSILLILRLPFCGNHVIPHTYCEHMGLAHLSCASIKINIIYGLCAICNLVF
DITVIALSYVHILCAVFRLPTHEARLKSLSTCGSHVCVILAFYTPALFSFMTHCFGRNVPRYIHILLANL
YVVVPPMLNPVIYGVRTKQIYKCVKKILLQEQGMEKEEYLIHTRF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001005164
ORF Size 975 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001005164.1, NP_001005164.1
RefSeq Size 978 bp
RefSeq ORF 978 bp
Locus ID 119678
UniProt ID Q8NGJ4
Cytogenetics 11p15.4
Protein Families Transmembrane
Protein Pathways Olfactory transduction
MW 36.4 kDa
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001005164" in other vectors (4)
SKU Description Size Price
RC217838L3 Lenti ORF clone of Human olfactory receptor, family 52, subfamily E, member 2 (OR52E2), Myc-DDK-tagged 10 ug
$600.00
RC217838L4 Lenti ORF clone of Human olfactory receptor, family 52, subfamily E, member 2 (OR52E2), mGFP tagged 10 ug
$600.00
RG217838 OR52E2 (tGFP-tagged) - Human olfactory receptor, family 52, subfamily E, member 2 (OR52E2) 10 ug
$500.00
SC300805 OR52E2 (untagged)-Human olfactory receptor, family 52, subfamily E, member 2 (OR52E2) 10 ug
$330.00

Plasmid Map for RC217838

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC217838
Circular map for RC217838

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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