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TDRKH (NM_001083964) Human Tagged ORF Clone

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SKU
RC217780
TDRKH (Myc-DDK-tagged)-Human tudor and KH domain containing (TDRKH), transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $480.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TDRKH
Synonyms TDRD2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217780 representing NM_001083964
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTACTGAACGGACTTCTTGGACAAGCCTGTCCACCATTCAGAAAATAGCCCTGGGCCTTGGGATCC
CAGCCAGTGCAACAGTTGCCTATATCCTATACCGCAGGTATAGGGAAAGCAGAGAAGAGCGGCTGACATT
TGTTGGGGAAGATGACATTGAGATAGAGATGCGGGTTCCCCAGGAGGCTGTGAAACTCATCATTGGCCGG
CAAGGAGCCAATATTAAACAGCTGCGGAAACAGACAGGTGCTCGGATTGATGTGGACACAGAGGATGTAG
GCGATGAGCGAGTGCTGCTTATCAGTGGTTTTCCTGTTCAGGCATCTGGAGCCAAAATTACCTGTGACAA
AGAATCAGAAGGGACATTACTACTATCAAGACTTATAAAAATCTCAGGAACACAGAAGGAAGTGGCAGCA
GCCAAGCATTTGATACTGGAGAAAGTTTCAGAAGATGAAGAACTTCGGAAGAGAATTGCTCATTCTGCAG
AAACCAGGGTCCCACGCAAACAGCCAATCAGTGTGAGAAGAGAAGACATGACAGAGCCAGGTGGAGCTGG
AGAGCCAGCATTATGGAAAAACACCAGTTCTAGCATGGAGCCGACTGCACCCCTGGTGACTCCTCCACCC
AAAGGAGGAGGCGACATGGCTGTGGTAGTGTCAAAGGAAGGTTCCTGGGAGAAACCTAGTGATGACAGCT
TTCAGAAGTCTGAAGCCCAGGCCATCCCAGAGATGCCCATGTTTGAAATCCCCAGTCCTGACTTCAGTTT
TCATGCTGATGAGTACCTAGAAGTCTACGTTTCTGCTTCTGAGCACCCTAACCACTTCTGGATCCAGATC
GTTGGCTCCCGCAGCCTGCAATTGGATAAGCTTGTCAATGAGATGACCCAGCACTATGAGAATAGTGTGC
CTGAAGACTTGACTGTGCATGTAGGAGACATTGTAGCAGCACCTTTACCTACAAATGGTTCCTGGTATCG
AGCCCGGGTCCTCGGCACCTTGGAGAATGGGAACTTGGACCTCTATTTTGTTGACTTTGGAGATAATGGA
GATTGCCCACTGAAGGACCTCAGGGCTCTCAGGAGTGACTTCCTAAGCCTTCCATTTCAAGCAATAGAAT
GTAGTCTGGCACGGATTGCTCCCTCAGGTGACCAGTGGGAAGAGGAAGCTTTGGATGAGTTTGATAGACT
CACTCATTGTGCTGACTGGAAGCCTCTGGTAGCCAAGATCTCTAGCTATGTCCAGACTGGGATCTCAACT
TGGCCAAAGATCTACTTATATGATACTAGCAATGGGAAGAAACTTGATATTGGGCTAGAATTAGTACACA
AAGGATACGCAATTGAGCTTCCTGAAGACATAGAAGAAAACAGAGCTGTCCCAGACATGTTGAAGGACAT
GGCCACAGAAACAGATGCCTCTCTCAGCACGTTGCTCACTGAGACCAAAAAGAGCTCTGGAGAGATAACA
CATACCCTGTCCTGCCTCAGCTTATCAGAAGCTGCTTCCATGTCTGGTGATGATAACCTTGAAGATGACT
ACTTACTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC217780 representing NM_001083964
Red=Cloning site Green=Tags(s)

MSTERTSWTSLSTIQKIALGLGIPASATVAYILYRRYRESREERLTFVGEDDIEIEMRVPQEAVKLIIGR
QGANIKQLRKQTGARIDVDTEDVGDERVLLISGFPVQASGAKITCDKESEGTLLLSRLIKISGTQKEVAA
AKHLILEKVSEDEELRKRIAHSAETRVPRKQPISVRREDMTEPGGAGEPALWKNTSSSMEPTAPLVTPPP
KGGGDMAVVVSKEGSWEKPSDDSFQKSEAQAIPEMPMFEIPSPDFSFHADEYLEVYVSASEHPNHFWIQI
VGSRSLQLDKLVNEMTQHYENSVPEDLTVHVGDIVAAPLPTNGSWYRARVLGTLENGNLDLYFVDFGDNG
DCPLKDLRALRSDFLSLPFQAIECSLARIAPSGDQWEEEALDEFDRLTHCADWKPLVAKISSYVQTGIST
WPKIYLYDTSNGKKLDIGLELVHKGYAIELPEDIEENRAVPDMLKDMATETDASLSTLLTETKKSSGEIT
HTLSCLSLSEAASMSGDDNLEDDYLL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001083964
ORF Size 1548 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001083964.1, NP_001077433.1
RefSeq Size 2719 bp
RefSeq ORF 1551 bp
Locus ID 11022
UniProt ID Q9Y2W6
Cytogenetics 1q21.3
Protein Families Transmembrane
MW 57 kDa
Summary Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001083964" in other vectors (4)
SKU Description Size Price
RC217780L3 Lenti-ORF clone of TDRKH (Myc-DDK-tagged)-Human tudor and KH domain containing (TDRKH), transcript variant 4 10 ug
$780.00
RC217780L4 Lenti-ORF clone of TDRKH (mGFP-tagged)-Human tudor and KH domain containing (TDRKH), transcript variant 4 10 ug
$780.00
RG217780 TDRKH (tGFP-tagged) - Human tudor and KH domain containing (TDRKH), transcript variant 4 10 ug
$680.00
SC316232 TDRKH (untagged)-Human tudor and KH domain containing (TDRKH), transcript variant 4 10 ug
$529.00

Plasmid Map for RC217780

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC217780
Circular map for RC217780

file-alt Citations

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