KIF25 (NM_005355) Human Tagged ORF Clone

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SKU
RC217730
KIF25 (Myc-DDK-tagged)-Human kinesin family member 25 (KIF25), transcript variant 2
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KIF25
Synonyms KNSL3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217730 representing NM_005355
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACATGGACCTCAGGTCAGCTTCAGCGTGAGAAGCAGGCCAGGCCTGGGTCTGGAGCCGTCCTGGCCT
TCCCAGATGACAAGGACCTCAGGGTTTATGGTCCAGCAGAGTCTCAGAGCGCGGTCTTTGGAGATGTGTG
CCCCCTACTCACTTCTCTCTTGGATGGGTACAATGTTTGTGTTATGGCGTATGGACAGACGGGCAGCGGA
AAGAGCTATACCATGCTGGGACGCCATTCGGACGACGGCCCTGTTCTGCCGCTTGACCCACAGAGTGACT
TAGGAATTATCCCTAGAGTGGCTGAGGAGCTCTTCAGGCTTATTTTGGAAAATACCTCAAGAAGCCCAAA
GGTTGAAGTCTCCATAGTGGAAGTTTACAATAATGACATTTTTGACCTTCTGGCCAAAGACAGCATTGCA
GCAGTGTCGGGGGTCAAGCGTGAGGTGGTGACAGCCAAGGATGGACGGACAGAGGTTGCGCTGCTGGCCT
CTGAGGCTGTCGGCAGCGCCTCGAAACTGATGGAGCTCGTTCATGGAGGTCTGCAGCTCAGGGCGAAGCA
CCCCACCCTGGTGCACGCGGATTCCTCCAGGTCTCACCTGATAATTACGGTGACTCTAACCACAGCCTCC
TGCTCTGACAGCACTGCAGACCAAGCCTGCAGTGCCACCCTCCCCAGGGAGCAAACAGAGGCAGGAAGGG
CAGGAAGGAGCCGCAGAGCTTCTCAAGGGGCCTTGGCTCCACAGCTGGTTCCTGGGAACCCCGCAGGGCA
TGCGGAGCAGGTGCAGGCTCGACTACAGCTCGTGGACTCGGCCGGCAGCGAGTGCGTTGGAGGCGATGCG
AAGTTACTGGTGATTCTCTGCATTTCTCCCAGCCAGAGGCACCTGGCACAGACGTTGCAGGGCCTGGGTT
TCGGGATCCGAGCTCGGCAAGTCCAGCGAGGCCCTGCCCGAAAGAAGCCGCCCAGCTCCCAAACGGAGGG
GAAGAGGAGGCCGGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC217730 representing NM_005355
Red=Cloning site Green=Tags(s)

MTWTSGQLQREKQARPGSGAVLAFPDDKDLRVYGPAESQSAVFGDVCPLLTSLLDGYNVCVMAYGQTGSG
KSYTMLGRHSDDGPVLPLDPQSDLGIIPRVAEELFRLILENTSRSPKVEVSIVEVYNNDIFDLLAKDSIA
AVSGVKREVVTAKDGRTEVALLASEAVGSASKLMELVHGGLQLRAKHPTLVHADSSRSHLIITVTLTTAS
CSDSTADQACSATLPREQTEAGRAGRSRRASQGALAPQLVPGNPAGHAEQVQARLQLVDSAGSECVGGDA
KLLVILCISPSQRHLAQTLQGLGFGIRARQVQRGPARKKPPSSQTEGKRRPD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005355
ORF Size 996 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005355.3, NP_005346.3
RefSeq Size 1354 bp
RefSeq ORF 999 bp
Locus ID 3834
UniProt ID Q9UIL4
Cytogenetics 6q27
Protein Families Druggable Genome
MW 35.1 kDa
Summary The protein encoded by this gene is a member of the kinesin-like protein family. Protein family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. However, the particular function of this gene product has not yet been determined. Two alternatively spliced transcript variants which encode products have been described. Other splice variants have been found that lack exon 2 and the initiation codon for translation. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_005355" in other vectors (4)
SKU Description Size Price
RC217730L3 Lenti ORF clone of Human kinesin family member 25 (KIF25), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC217730L4 Lenti ORF clone of Human kinesin family member 25 (KIF25), transcript variant 2, mGFP tagged 10 ug
$600.00
RG217730 KIF25 (tGFP-tagged) - Human kinesin family member 25 (KIF25), transcript variant 2 10 ug
$489.00 $500.00
SC303623 KIF25 (untagged)-Human kinesin family member 25 (KIF25), transcript variant 2 10 ug
$330.00

Plasmid Map for RC217730

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Cloning scheme for RC217730

Circular map for RC217730

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