AFF4 (NM_014423) Human Tagged ORF Clone
$1,012.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | AFF4 |
| Synonyms | AF5Q31; CHOPS; MCEF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014423 |
| ORF Size | 3489 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014423.4 |
| RefSeq Size | 9528 bp |
| RefSeq ORF | 3492 bp |
| Locus ID | 27125 |
| UniProt ID | Q9UHB7 |
| Cytogenetics | 5q31.1 |
| Domains | AF-4 |
| Protein Families | Transcription Factors |
| MW | 127.3 kDa |
| Summary | The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). provided by RefSeq, Oct 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014423" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC217692L1 | Lenti ORF clone of Human AF4/FMR2 family, member 4 (AFF4), Myc-DDK-tagged | 10 ug |
$1,312.00
|
|
| RC217692L2 | Lenti ORF clone of Human AF4/FMR2 family, member 4 (AFF4), mGFP tagged | 10 ug |
$1,312.00
|
|
| RC217692L3 | Lenti ORF clone of Human AF4/FMR2 family, member 4 (AFF4), Myc-DDK-tagged | 10 ug |
$1,312.00
|
|
| RC217692L4 | Lenti ORF clone of Human AF4/FMR2 family, member 4 (AFF4), mGFP tagged | 10 ug |
$1,312.00
|
|
| RG217692 | AFF4 (tGFP-tagged) - Human AF4/FMR2 family, member 4 (AFF4) | 10 ug |
$789.00
$1,212.00
|
|
| SC108598 | AFF4 (untagged)-Human AF4/FMR2 family, member 4 (AFF4) | 10 ug |
$1,013.00
|
|
Citations
Clinical and molecular spectrum of CHOPS syndrome
Raible, Mehta, Bettale et al
Am. J. Med. Genet. A (2019)
Raible, Mehta, Bettale et al
Am. J. Med. Genet. A (2019)
AI Summary: OriGene products, specifically the Myc-DDK-tagged AFF4 plasmid (RC217692) and the Myc-DDK-tagged SIAH1a plasmid (RC206576), were used to study the functional effects of AFF4 mutations associated with CHOPS syndrome in this research.
Snippet: A plasmid encoding Myc‐DDK‐tagged AFF4 (RC217692) was obtained from OriGene Technologies. This plasmid, containing the AFF4 variant T254A, as well as a HA‐His‐tagged SIAH1a plasmid generated from a plasmid encoding Myc‐DDK‐tagged SIAH1a (Origene Technologies, RC206576), were used (Izumi et al., 2015)
Germline gain-of-function mutations in AFF4 cause a developmental syndrome functionally linking the super elongation complex and cohesin
Izumi, Nakato, Zhang et al
Nat. Genet. (2015) 47 (4), 338-44 DOI: 10.1038/ng.3229
Izumi, Nakato, Zhang et al
Nat. Genet. (2015) 47 (4), 338-44 DOI: 10.1038/ng.3229
Cited by 149
AI Summary: OriGene products contributed to this study by providing Myc-DDK-tagged AFF4 (RC217692) and Myc-DDK-tagged SIAH1a (RC206576) plasmids, as well as the RapidShuttling kit (PS200008) used for generating tagged constructs, which facilitated functional analyses of the implicated proteins in the described developmental syndrome.
Snippet: … Antibodies to ELL2 used for immunoblotting (A302-505A) and AFF4 used for ChIP-seq analysis (A302-538A) were purchased from Bethyl Lab. Antibodies to NIPBL (sc-374625) and cyclin T1 (sc-10750) were from Santa Cruz Biotechnology. …Plasmids encoding MycDDK-tagged AFF4 (RC217692) and Myc-DDK-tagged SIAH1a (RC206576) were obtained from OriGene Technologies. To generate HA-His-tagged SIAH1a, the SIAH1A cDNA was subcloned into pCMV6-AC-HA-His, using the OriGene RapidShuttling kit (SfgI-MluI, PS200008) according to the manufacturer’s directions. Mutagenesis of the AFF4 expression plasmid to introduce each of the nonsynonymous variants was performed using the QuikChange Site-Directed Mutagenesis kit by Agilent Technologies following the manufacturer’s protocol. …
Showing 1-2 of 2 papers.
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