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LRMDA (NM_032024) Human Tagged ORF Clone

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SKU
RC217685
C10orf11 (Myc-DDK-tagged)-Human chromosome 10 open reading frame 11 (C10orf11)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LRMDA
Synonyms C10orf11; CDA017
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217685 representing NM_032024
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAAGTATTTGTCACTCAGCGGCAATCATTCTTCAAATAAAAGGTCACTGGAAGGACTGAGCGCAT
TCAGGAGCCTGGAGGAACTCATCTTGGACAACAATCAGCTGGGGGACGACCTTGTGTTGCCAGGGTTACC
CAGACTGCATACCTTAACCCTCAACAAGAACCGAATCACTGATTTGGAGAACCTGCTGGATCACTTGGCA
GAAGTGACACCAGCTCTGGAGTACCTCAGTCTGCTGGGCAACGTGGCCTGTCCCAACGAGCTGGTCAGCT
TGGAAAAGGATGAGGAAGACTACAAGAGATACAGATGCTTTGTTCTGTACAAGCTGCCCAACTTGAAATT
TCTGGATGCCCAGAAAGTAACCAGACAAGAACGAGAGGAGGCGTTGGTCAGAGGAGTCTTCATGAAGGTG
GTGAAGCCCAAGGCTTCTAGTGAGGACGTTGCCAGCTCCCCGGAGCGCCACTACACGCCCTTGCCTTCTG
CTTCCAGGGAACTCACCAGTCACCAAGGTGTCCTGGGGAAGTGTCGCTACGTTTACTATGGGAAAAACTC
AGAGGGCAACAGGTTTATCCGAGATGACCAGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC217685 representing NM_032024
Red=Cloning site Green=Tags(s)

MEKYLSLSGNHSSNKRSLEGLSAFRSLEELILDNNQLGDDLVLPGLPRLHTLTLNKNRITDLENLLDHLA
EVTPALEYLSLLGNVACPNELVSLEKDEEDYKRYRCFVLYKLPNLKFLDAQKVTRQEREEALVRGVFMKV
VKPKASSEDVASSPERHYTPLPSASRELTSHQGVLGKCRYVYYGKNSEGNRFIRDDQL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032024
ORF Size 594 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032024.4, NP_114413.1
RefSeq Size 902 bp
RefSeq ORF 597 bp
Locus ID 83938
UniProt ID Q9H2I8
Cytogenetics 10q22.2-q22.3
MW 22.4 kDa
Summary This gene encodes a leucine-rich repeat protein. The encoded protein is thought to play a role in melanocyte differentiation. Mutations in this gene have been associated with autosomal recessive oculocutaneous albinism 7 (OCA7). Alternatively spliced transcript variants have been identified. provided by RefSeq, Mar 2015
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"NM_032024" in other vectors (4)
SKU Description Size Price
RC217685L3 Lenti ORF clone of Human chromosome 10 open reading frame 11 (C10orf11), Myc-DDK-tagged 10 ug
$600.00
RC217685L4 Lenti ORF clone of Human chromosome 10 open reading frame 11 (C10orf11), mGFP tagged 10 ug
$600.00
RG217685 C10orf11 (tGFP-tagged) - Human chromosome 10 open reading frame 11 (C10orf11) 10 ug
$500.00
SC305386 C10orf11 (untagged)-Human chromosome 10 open reading frame 11 (C10orf11) 10 ug
$330.00

Plasmid Map for RC217685

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC217685
Circular map for RC217685

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