DAAM1 (NM_014992) Human Tagged ORF Clone
SKU
RC217675
DAAM1 (Myc-DDK-tagged)-Human dishevelled associated activator of morphogenesis 1 (DAAM1)
$938.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | DAAM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014992 |
| ORF Size | 3234 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014992.2 |
| RefSeq Size | 4256 bp |
| RefSeq ORF | 3237 bp |
| Locus ID | 23002 |
| UniProt ID | Q9Y4D1 |
| Cytogenetics | 14q23.1 |
| Domains | FH2 |
| Protein Pathways | Wnt signaling pathway |
| MW | 123.3 kDa |
| Summary | Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins. provided by RefSeq, Jul 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014992" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC217675L1 | Lenti ORF clone of Human dishevelled associated activator of morphogenesis 1 (DAAM1), Myc-DDK-tagged | 10 ug |
$1,238.00
|
|
| RC217675L2 | Lenti ORF clone of Human dishevelled associated activator of morphogenesis 1 (DAAM1), mGFP tagged | 10 ug |
$1,238.00
|
|
| RC217675L3 | Lenti ORF clone of Human dishevelled associated activator of morphogenesis 1 (DAAM1), Myc-DDK-tagged | 10 ug |
$1,238.00
|
|
| RC217675L4 | Lenti ORF clone of Human dishevelled associated activator of morphogenesis 1 (DAAM1), mGFP tagged | 10 ug |
$1,238.00
|
|
| RG217675 | DAAM1 (tGFP-tagged) - Human dishevelled associated activator of morphogenesis 1 (DAAM1) | 10 ug |
$789.00
$1,138.00
|
|
| SC120568 | DAAM1 (untagged)-Human dishevelled associated activator of morphogenesis 1 (DAAM1) | 10 ug |
$939.00
|
|
Citations
A global transcriptional network connecting noncoding mutations to changes in tumor gene expression
Zhang, Bojorquez-Gomez, Velez et al
Nat. Genet. (2018) 50 (4), 613-620 DOI: 10.1038/s41588-018-0091-2
Zhang, Bojorquez-Gomez, Velez et al
Nat. Genet. (2018) 50 (4), 613-620 DOI: 10.1038/s41588-018-0091-2
AI Summary: OriGene’s promoter-less GFP plasmid (pRMT-tGFP) was used as a negative control in flow cytometry experiments to assess the effects of noncoding mutations on MTG2 gene expression in this study.
Snippet: … Cells with typical forward (size) and side (granularity) scatter properties were further analyzed for GFP expression. As a negative control, cells were transfected with an empty lentiGuide-Puro plasmid (Addgene) for the DAAM1 experiments (Figs. 3c, d;Supplementary Fig. 4) or a promoter-less GFP plasmid (pRMT-tGFP, Origene) for the MTG2 experiments (Fig. 4b)
LRP6 exerts non-canonical effects on Wnt signaling during neural tube closure
Gray, Kholmanskikh, Castaldo et al
Hum. Mol. Genet. (2013)
Gray, Kholmanskikh, Castaldo et al
Hum. Mol. Genet. (2013)
Cited by 47
Showing 1-2 of 2 papers.
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