FOXP3 (NM_014009) Human Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | FOXP3 |
| Synonyms | AIID; DIETER; IPEX; JM2; PIDX; XPID |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014009 |
| ORF Size | 1293 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014009.4 |
| RefSeq Size | 1869 bp |
| RefSeq ORF | 1296 bp |
| Locus ID | 50943 |
| UniProt ID | Q9BZS1 |
| Cytogenetics | Xp11.23 |
| Protein Families | Transcription Factors |
| MW | 47.1 kDa |
| Summary | The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014009" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC217580L1 | Lenti ORF clone of Human forkhead box P3 (FOXP3), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC217580L2 | Lenti ORF clone of Human forkhead box P3 (FOXP3), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RC217580L3 | Lenti ORF clone of Human forkhead box P3 (FOXP3), transcript variant 1, Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC217580L4 | Lenti ORF clone of Human forkhead box P3 (FOXP3), transcript variant 1, mGFP tagged | 10 ug |
$986.00
|
|
| RG217580 | FOXP3 (tGFP-tagged) - Human forkhead box P3 (FOXP3), transcript variant 1 | 10 ug |
$886.00
|
|
| SC310403 | FOXP3 (untagged)-Human forkhead box P3 (FOXP3), transcript variant 1 | 10 ug |
$457.00
|
|
Citations
Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1β-dependent response of Th17 cells
Kim, Kim, Cho et al
Elife (2021) 10 DOI: 10.7554/eLife.61841
Kim, Kim, Cho et al
Elife (2021) 10 DOI: 10.7554/eLife.61841
AI Summary: OriGene’s pCMV5-entry-FoxP3 plasmid was used as the source of human FoxP3 ORF, enabling the authors to generate lentiviral constructs for FoxP3 expression and investigate its regulatory role in IL-1RII transcription in CD4+ T cells.
Snippet: ha promoter-driven expression cassette and CMV promoter-GFP expression cassette (Lee et al., 2019) , by replacing GFP ORF with puroR ORF. Human FoxP3 ORF was amplified by PCR from pCMV5-entry-FoxP3 (OriGene, Rockville, MD, USA) and cloned into multi-cloning sites under the EF1alpha promoter in pLEF-puro. The lentivirus for FoxP3 expression was produced by transfecting pLEF-puro-FoxP3 plasmid into
Transcription factor FOXP3: A repressor of the TFPI gene?
Skretting, Andersen, Myklebust et al
J. Cell. Biochem. (2019)
Skretting, Andersen, Myklebust et al
J. Cell. Biochem. (2019)
AI Summary: OriGene products contributed to this study by providing the pCMV6-Entry plasmid containing human FOXP3 cDNA (Myc-DDK-tagged FOXP3, RC217580) and the empty plasmid (pCMV6-Neo), which were used for FOXP3 overexpression experiments to investigate its regulatory effect on TFPI gene expression.
Showing 1-2 of 2 papers.
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