SERHL2 (NM_014509) Human Tagged ORF Clone

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SKU
RC217568
SERHL2 (Myc-DDK-tagged)-Human serine hydrolase-like 2 (SERHL2)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SERHL2
Synonyms dJ222E13.1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217568 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGAGAACGCCGCACCAGGTCTGATCTCAGAGCTGAAGCTGGCTGTGCCCTGGGGCCACATCGCAG
CCAAAGCCTGGGGCTCCCTGCAGGGCCCTCCAGTTCTCTGCCTGCACGGCTGGCTGGACAATGCCAGCTC
CTTCGACAGACTCATCCCTCTTCTCCCGCAAGACTTTTATTACGTTGCCATGGATTTCGGAGGTCATGGG
CTCTCGTCCCATTACAGCCCAGGTGTCCCATATTACCTCCAGACTTTTGTGAGTGAGATCCGAAGAGTTG
TGGCAGCCTTGAAATGGAATCGATTCTCCATTCTGGGCCACAGCTTCGGTGGCGTCGTGGGCGGAATGTT
TTTCTGTACCTTCCCCGAGATGGTGGATAAACTTATCTTGCTGGACACGCCGCTCTTTCTCCTGGAATCA
GATGAAATGGAGAACTTGCTGACCTACAAGCGGAGAGCCATAGAGCACGTGCTGCAGGTAGAGGCCTCCC
AGGAGCCCTCGCACGTGTTCAGCCTGAAGCAGCTGCTGCAGAGGTTACTGAAGAGCAATAGCCACTTGAG
TGAGGAGTGCGGGGAGCTTCTCCTGCAAAGAGGAACCACGAAGGTGGCCACAGGTCTGGTTCTGAACAGA
GACCAGAGGCTCGCCTGGGCAGAGAACAGCATTGACTTCATCAGCAGGGAGCTGTGTGCGCATTCCATCA
GGAAGCTGCAGGCCCATGTCCTGTTGATCAAAGCAGTCCACGGATATTTTGATTCAAGACAGAATTACTC
TGAGAAGGAGTCCCTGTCGTTCATGATAGACACGATGAAATCCACCCTCAAAGAGCAGTTCCAGTTTGTG
GAAGTCCCAGGCAATCACTGTGTCCACATGAGCGAACCCCAGCACGTGGCCAGTATCATCAGCTCCTTCT
TACAGTGCACACACATGCTCCCAGCCCAGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC217568 protein sequence
Red=Cloning site Green=Tags(s)

MSENAAPGLISELKLAVPWGHIAAKAWGSLQGPPVLCLHGWLDNASSFDRLIPLLPQDFYYVAMDFGGHG
LSSHYSPGVPYYLQTFVSEIRRVVAALKWNRFSILGHSFGGVVGGMFFCTFPEMVDKLILLDTPLFLLES
DEMENLLTYKRRAIEHVLQVEASQEPSHVFSLKQLLQRLLKSNSHLSEECGELLLQRGTTKVATGLVLNR
DQRLAWAENSIDFISRELCAHSIRKLQAHVLLIKAVHGYFDSRQNYSEKESLSFMIDTMKSTLKEQFQFV
EVPGNHCVHMSEPQHVASIISSFLQCTHMLPAQL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014509
ORF Size 942 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014509.5
RefSeq Size 1447 bp
RefSeq ORF 945 bp
Locus ID 253190
UniProt ID Q9H4I8, A0A140VK89
Cytogenetics 22q13.2
MW 35.4 kDa
Summary Probable serine hydrolase. May be related to cell muscle hypertrophy.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_014509" in other vectors (4)
SKU Description Size Price
RC217568L3 Lenti ORF clone of Human serine hydrolase-like 2 (SERHL2), Myc-DDK-tagged 10 ug
$600.00
RC217568L4 Lenti ORF clone of Human serine hydrolase-like 2 (SERHL2), mGFP tagged 10 ug
$600.00
RG217568 SERHL2 (tGFP-tagged) - Human serine hydrolase-like 2 (SERHL2) 10 ug
$489.00 $500.00
SC304117 SERHL2 (untagged)-Human serine hydrolase-like 2 (SERHL2) 10 ug
$330.00

Plasmid Map for RC217568

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Cloning scheme for RC217568

Circular map for RC217568

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