TTF1 (NKX2-1) (NM_003317) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TTF1 |
| Synonyms | BCH; BHC; NK-2; NKX2.1; NKX2A; NMTC1; T/EBP; TEBP; TITF1; TTF-1; TTF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003317 |
| ORF Size | 1113 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003317.4 |
| RefSeq Size | 2352 bp |
| RefSeq ORF | 1116 bp |
| Locus ID | 7080 |
| UniProt ID | P43699 |
| Cytogenetics | 14q13.3 |
| Domains | homeobox |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors |
| MW | 38.4 kDa |
| Summary | This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. provided by RefSeq, Feb 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003317" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC217520L1 | Lenti ORF clone of Human NK2 homeobox 1 (NKX2-1), transcript variant 2, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC217520L2 | Lenti ORF clone of Human NK2 homeobox 1 (NKX2-1), transcript variant 2, mGFP tagged | 10 ug |
$757.00
|
|
| RC217520L3 | Lenti ORF clone of Human NK2 homeobox 1 (NKX2-1), transcript variant 2, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC217520L4 | Lenti ORF clone of Human NK2 homeobox 1 (NKX2-1), transcript variant 2, mGFP tagged | 10 ug |
$757.00
|
|
| RG217520 | NKX2 (tGFP-tagged) - Human NK2 homeobox 1 (NKX2-1), transcript variant 2 | 10 ug |
$489.00
$657.00
|
|
| SC118030 | NKX2 (untagged)-Human NK2 homeobox 1 (NKX2-1), transcript variant 2 | 10 ug |
$457.00
|
|
Citations
Consecutive Hypoxia Decreases Expression of NOTCH3, HEY1, CC10, and FOXJ1 via NKX2-1 Downregulation and Intermittent Hypoxia-Reoxygenation Increases Expression of BMP4, NOTCH1, MKI67, OCT4, and MUC5AC via HIF1A Upregulation in Human Bronchial Epithelial Cells
Yang, Lin, Wang et al
Front Cell Dev Biol (2020) 8, 572276 DOI: 10.3389/fcell.2020.572276
Yang, Lin, Wang et al
Front Cell Dev Biol (2020) 8, 572276 DOI: 10.3389/fcell.2020.572276
Cited by 10
AI Summary: In this study, OriGene products including HIF1A (SR307047), NKX2-1 (SR300238) siRNAs, and NKX2-1 cDNA (RC209685) were used to modulate gene expression in human bronchial epithelial cells, enabling the authors to demonstrate the regulatory roles of HIF1A and NKX2-1 in hypoxia-induced changes in key gene markers.
Showing 1-1 of 1 papers.
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