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TTF1 (NKX2-1) (NM_003317) Human Untagged Clone

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SKU
SC118030
NKX2 (untagged)-Human NK2 homeobox 1 (NKX2-1), transcript variant 2
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TTF1
Synonyms BCH; BHC; NK-2; NKX2.1; NKX2A; NMTC1; T/EBP; TEBP; TITF1; TTF-1; TTF1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118030 sequence for NM_003317 edited (data generated by NextGen Sequencing)
ATGTCGATGAGTCCAAAGCACACGACTCCGTTCTCAGTGTCTGACATCTTGAGTCCCCTG
GAGGAAAGCTACAAGAAAGTGGGCATGGAGGGCGGCGGCCTCGGGGCTCCGCTGGCGGCG
TACAGGCAGGGCCAGGCGGCACCGCCAACAGCGGCCATGCAGCAGCACGCCGTGGGGCAC
CACGGCGCCGTCACCGCCGCCTACCACATGACGGCGGCGGGGGTGCCCCAGCTCTCGCAC
TCCGCCGTGGGGGGCTACTGCAACGGCAACCTGGGCAACATGAGCGAGCTGCCGCCGTAC
CAGGACACCATGAGGAACAGCGCCTCTGGCCCCGGATGGTACGGCGCCAACCCAGACCCG
CGCTTCCCCGCCATCTCCCGCTTCATGGGCCCGGCGAGCGGCATGAACATGAGCGGCATG
GGCGGCCTGGGCTCGCTGGGGGACGTGAGCAAGAACATGGCCCCGCTGCCAAGCGCGCCG
CGCAGGAAGCGCCGGGTGCTCTTCTCGCAGGCGCAGGTGTACGAGCTGGAGCGACGCTTC
AAGCAACAGAAGTACCTGTCGGCGCCGGAGCGCGAGCACCTGGCCAGCATGATCCACCTG
ACGCCCACGCAGGTCAAGATCTGGTTCCAGAACCACCGCTACAAAATGAAGCGCCAGGCC
AAGGACAAGGCGGCGCAGCAGCAACTGCAGCAGGACAGCGGCGGCGGCGGGGGCGGCGGG
GGCACCGGGTGCCCGCAGCAGCAACAGGCTCAGCAGCAGTCGCCGCGACGCGTGGCGGTG
CCGGTCCTGGTGAAAGACGGCAAACCGTGCCAGGCGGGTGCCCCCGCGCCGGGCGCCGCC
AGCCTACAAGGCCACGCGCAGCAGCAGGCGCAGCACCAGGCGCAGGCCGCGCAGGCGGCG
GCAGCGGCCATCTCCGTGGGCAGCGGTGGCGCCGGCCTTGGCGCACACCCGGGCCACCAG
CCAGGCAGCGCAGGCCAGTCTCCGGACCTGGCGCACCACGCCGCCAGCCCCGCGGCGCTG
CAGGGCCAGGTATCCAGCCTGTCCCACCTGAACTCCTCGGGCTCGGACTACGGCACCATG
TCCTGCTCCACCTTGCTATACGGTCGGACCTGGTGA

Clone variation with respect to NM_003317.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003317 unedited
NGTCGCGATTAGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCTGACAGAC
ACGTAGACCAACAGTGCGGCCCCAGGTTCGTCCCCAGACTCGCTCGCTCATTTGTTGGCG
ACTGGGGCTCAGCGCAGCGAAGCCCGATGTGGTCCGGAGGCAGTGGGAAGGCGCGGGGCT
GGGAGGCCGCGGCGGGAGGGAGGAGCAGCCCCGGCAGGCTCAGCCGCCGCCGAATCATGT
CGATGAGTCCAAAGCACACGACTCCGTTCTCAGTGTCTGACATCTTGAGTCCCCTGGAGG
AAAGCTACAAGAAAGTGGGCATGGAGGGCGGCGGCCTCGGGGCTCCGCTGGCGGCGTACA
GGCAGGGCCAGGCGGCACCGCCAACAGCGGCCATGCAGCAGCACGCCGTGGGGCACCACG
GCGCCGTCACCGCCGCCTACCACATGACGGCGGCGGGGGTGCCCCAGCTCTCGCACTCCG
CCGTGGGGGGCTACTGCAACGGCAACCTGGGCAACATGAGCGAGCTGCCGCCGTACCAGG
ACACCATGAGGAACAGCGCCTCTGGCCCCGGATGGTACGGCGCCAACCCAGACCCGCGCT
TCCCCGCCATCTCCCGCTTCATGGGCCCGGCGAGCGGCATGAACATGAGCGGCATGGGCG
GCCTGNGCTCGCTGGGGGACGTGAGCAAGAACATGGCCCCGCTGCCAAGCGCGCCGCGCA
GGAAGCGCCGGGTGCTCTTCTCGCAGGCGCAGGTGTACGAGCTGGAGCGACGCTTCAAGC
AACAGAAGTACCCTGTCGCGCCGGAGCGCGAGCACCTGGCCAGCATGATCCACCTGACGC
CCACGCAGTCAGATCTGGTTCCAGAACCACCGCTACANATGAAGCGCCAGNNCAGGNACA
NGGCNGCGCAGCAGCAACTGCAGCAGGACAGC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003317 unedited
GCGTTAAGGAGACTTTATTATTTTTTTAATTTATTAGNAGCTTCTTAGGGACTATATTTA
AGCCAAATATCTACATAAGTTACAACAGAAAAAGATGACGCCGCAAATACCAAACTGCCA
AATAATATACACAGATTTGTCAATGCCCATAAAAAATGTGAAGGGCTGGGGACTGGGAGT
GGTTTTTCTTTTTACAACAAAATGTACAGATTACTAAAAACTAGGCATTTAGTCCAACTT
TTGACAGCGTTTTACAGCTACAAGTTCACATTAAACAAACTATTTTCGCGGAGGGCGGTC
GCCGCTGAGCCTAGGCGGCCAGAGGGTGCCGGGGAGGGGGCACTTCCTTTGTGTCAGTGA
CAAGTGGGTTATGTTGAAGACTCTTTCCTCTCCCCAGCTCCCGTCCTCCCTTCAAAAAAA
AAAAAAAAATCCTGGTATTTACAAGCGAGTCCTCTTTGCTGGCAGAGTGTGCCCAGAGTG
AAGTTTGGTCTTTAGAGTCCAGAGCCATGTCAGCACAGAGCCCTCTCCAATCTGTGCCCC
GCCCCTAGCGTGGAAAACCCATTTGAATCACCAAAAAAAGACACCCCAAAGCTGTTTTAT
GCCCTTTTCTGCTTAAAGATTCCTTGAGATTGGATGCGCTTGGCTGCTCTTCATTTTCTT
TTTTTTAAAAAAAAAAAACCCCCAAATTTTCAGGGGGGGAAAAAAACAAAGAAGTCCCAC
CACTTAGGCCTTTGTGGTTTTTTTGTTCCTTGGGCCTAAAACCGGCCCGNTTCTAAAGAA
AATTTAACGCGTTGGAACAACGGTGGATGGTGCTTCTTTGGCTGCCAGAAGGAACCGTGA
AGCCAACCCTGGGCTTAGGCCGACCGGTGTCTTTTACCAATTCCAACCGTTTCAAGGTGT
ACCGAATTGGGCCCCTTTCCACACCAG
Restriction Sites NotI-NotI |
ACCN NM_003317
Insert Size 2300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003317.3, NP_003308.1
RefSeq Size 2352 bp
RefSeq ORF 1116 bp
Locus ID 7080
UniProt ID P43699
Cytogenetics 14q13.3
Domains homeobox
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
Summary This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. provided by RefSeq, Feb 2014
Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1.
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Documents
Resources
"NM_003317" in other vectors (6)
SKU Description Size Price
RC217520 NKX2 (Myc-DDK-tagged)-Human NK2 homeobox 1 (NKX2-1), transcript variant 2 10 ug
$457.00
RC217520L1 Lenti ORF clone of Human NK2 homeobox 1 (NKX2-1), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC217520L2 Lenti ORF clone of Human NK2 homeobox 1 (NKX2-1), transcript variant 2, mGFP tagged 10 ug
$757.00
RC217520L3 Lenti ORF clone of Human NK2 homeobox 1 (NKX2-1), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC217520L4 Lenti ORF clone of Human NK2 homeobox 1 (NKX2-1), transcript variant 2, mGFP tagged 10 ug
$757.00
RG217520 NKX2 (tGFP-tagged) - Human NK2 homeobox 1 (NKX2-1), transcript variant 2 10 ug
$489.00 $657.00

file-alt Citations

Gamma-interferon-inducible, lysosome/endosome-localized thiolreductase, GILT, has anti-retroviral activity and its expression is counteracted by HIV-1
Kubo, Izumida, Yashima et al
Oncotarget (2016) 7 (44), 71255-71273 DOI: 10.18632/oncotarget.12104
Snippet: For co - transfection assays, the following expression vectors were used: pCMV6 - XL4 - Nkx2.1 109 and its control vector pCMV6 - XL4, provided from OriGene;pcDNA3 .1 - TP53 and its control 110 vector pcDNA3.1, constructed from pC53 - C1N, g enerously gifted by Pr D. Reisman 111 (University of South Carolina)
CCSP G38A polymorphism-environment interactions regulate CCSP levels differentially in COPD
Knabe, Varilh, Bergougnoux et al
Am. J. Physiol. Lung Cell Mol. Physiol. (2016)
Cited by 12
AI Summary: OriGene products pCMV6-XL4-Nkx2.1 and its control vector pCMV6-XL4 were used for co-transfection assays to study the regulatory effects of the Nkx2.1 transcription factor on CCSP promoter activity in the context of the CCSP G38A polymorphism.
Snippet: 229bp-38A and 926bp-38G, 926bp-38A. 140 Page 7. - 6 - For co-transfection assays, the following expression vectors were used: pCMV6-XL4-Nkx2.1 141 and its control vector pCMV6-XL4, provided from OriGene;pcDNA3.1-TP53 and its control 142 ... Cite SaveSaving
Integrative Genomics Implicates EGFR as a Downstream Mediator in NKX2-1 Amplified Non-Small Cell Lung Cancer
Clarke, Biscocho, Kwei et al
PLoS ONE (2015) 10 (11), e0142061 DOI: 10.1371/journal.pone.0142061
Cited by 20
AI Summary: OriGene contributed a full-length NKX2-1 cDNA clone (in pOTB7), which was used to study NKX2-1 isoform expression in the investigation of its role in non-small cell lung cancer.
Snippet: All cell lines were grown at 37°C in RPMI-1640 medium with L-glutamate, supplemented with 10% (vol/vol) fetal bovine serum and 1X Pen/Strep. NKX2-1 isoform expression. A full-length NKX2-1 cDNA clone (in pOTB7) was obtained from Origene. ... Cite SaveSaving
FigureFigureFigure
Showing 1-3 of 3 papers.
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