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RPS21 (NM_001024) Human Tagged ORF Clone

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SKU
RC217512
RPS21 (Myc-DDK-tagged)-Human ribosomal protein S21 (RPS21)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RPS21
Synonyms HLDF; S21
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217512 representing NM_001024
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAACGACGCCGGCGAGTTCGTGGACCTGTACGTGCCGCGGAAATGCTCCGCTAGCAATCGCATCA
TCGGTGCCAAGGACCACGCATCCATCCAGATGAACGTGGCCGAGGTTGACAAGGTCACAGGCAGGTTTAA
TGGCCAGTTTAAAACTTATGCTATCTGCGGGGCCATTCGTAGGATGGGTGAGTCAGATGATTCCATTCTC
CGATTGGCCAAGGCCGATGGCATCGTCTCAAAGAACTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC217512 representing NM_001024
Red=Cloning site Green=Tags(s)

MQNDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSIL
RLAKADGIVSKNF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001024
ORF Size 249 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001024.4
RefSeq Size 418 bp
RefSeq ORF 252 bp
Locus ID 6227
UniProt ID P63220, Q6FGH5
Cytogenetics 20q13.33
Domains Ribosomal_S21e
Protein Pathways Ribosome
MW 8.9 kDa
Summary Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S21E family of ribosomal proteins. It is located in the cytoplasm. Alternative splice variants that encode different protein isoforms have been described, but their existence has not been verified. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. provided by RefSeq, Jul 2008
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"NM_001024" in other vectors (6)
SKU Description Size Price
RC217512L1 Lenti ORF clone of Human ribosomal protein S21 (RPS21), Myc-DDK-tagged 10 ug
$450.00
RC217512L2 Lenti ORF clone of Human ribosomal protein S21 (RPS21), mGFP tagged 10 ug
$450.00
RC217512L3 Lenti ORF clone of Human ribosomal protein S21 (RPS21), Myc-DDK-tagged 10 ug
$450.00
RC217512L4 Lenti ORF clone of Human ribosomal protein S21 (RPS21), mGFP tagged 10 ug
$450.00
RG217512 RPS21 (tGFP-tagged) - Human ribosomal protein S21 (RPS21) 10 ug
$350.00
SC119532 RPS21 (untagged)-Human ribosomal protein S21 (RPS21) 10 ug
$150.00

Plasmid Map for RC217512

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC217512
Circular map for RC217512

file-alt Citations

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