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CCDC153 (NM_001033658) Human Tagged ORF Clone

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SKU
RC217435
CCDC153 (Myc-DDK-tagged)-Human coiled-coil domain containing 153 (CCDC153)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCDC153
Synonyms coiled-coil domain containing 153; MGC125447; MGC125447, MGC125448; MGC125448
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217435 representing NM_001033658
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTCGCCAGTGCCATGCCCTGCAGGAGGATATGCAAACCCACAGCAAGCAGCTGGAGGAAGAAGTCA
AAGGCCTTCGGGGGCAGCTAGAGGCATGCCAAAGGGAGGCTGCAGCTGCCCGGGAAGAGGCTGAACAAGC
TCTCGGAGAGCGGGACCAGGCCCTGGCTCAGCTTCGGGCCCACATGGCAGACATGGAGGCGAAGTATGAG
GAAATCTTACACGACAGTCTGGACAGGCTCTTGGCCAAGCTGAGAGCCATCAAGCAGCAGTGGGATGGGG
CAGCACTGAGACTTCACGCCAGGCACAAGGAGCAGCAACGCCAGTTTGGACTCACCCCCCCTGGATCTTT
GAGGCCACCAGCCCCTAGTCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC217435 representing NM_001033658
Red=Cloning site Green=Tags(s)

MSRQCHALQEDMQTHSKQLEEEVKGLRGQLEACQREAAAAREEAEQALGERDQALAQLRAHMADMEAKYE
EILHDSLDRLLAKLRAIKQQWDGAALRLHARHKEQQRQFGLTPPGSLRPPAPSL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033658
ORF Size 372 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033658.1, NP_001028830.1
RefSeq Size 625 bp
RefSeq ORF 374 bp
Locus ID 283152
Cytogenetics 11q23.3
MW 13.9 kDa
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"NM_001033658" in other vectors (3)
SKU Description Size Price
RC217435L3 Lenti ORF clone of Human coiled-coil domain containing 153 (CCDC153), Myc-DDK-tagged 10 ug
$450.00
RC217435L4 Lenti ORF clone of Human coiled-coil domain containing 153 (CCDC153), mGFP tagged 10 ug
$450.00
RG217435 CCDC153 (tGFP-tagged) - Human coiled-coil domain containing 153 (CCDC153) 10 ug
$350.00

Plasmid Map for RC217435

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC217435
Circular map for RC217435

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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