PTPN5 (NM_001039970) Human Tagged ORF Clone

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SKU
RC217419
PTPN5 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) (PTPN5), transcript variant 3
  $496.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PTPN5
Synonyms PTPSTEP; STEP; STEP61
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217419 representing NM_001039970
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTATGAGGGAGCCAGGAGTGAGAGAGAGAACCACGCTGCTGATGACTCCGAGGGAGGGGCCCTGG
ACATGTGCTGCAGTGAGAGGCTACCGGGTCTCCCCCAGCCGATAGTGATGGAGGCACTGGACGAGGCTGA
AGGGCTCCAGGACTCACAGAGAGAGATGCCGCCACCCCCTCCTCCCTCGCCGCCCTCAGATCCAGCTCAG
AAGCCACCACCTCGAGGCGCTGGGAGCCACTCCCTCACTGTCAGGAGCAGCCTGTGCCTGTTCGCTGCCT
CACAGTTCCTGCTGGAACCCACGGCCTGGCTTGACTCTGGGACGTGGGGAGTCCCCAGTCTGCTGCTGGT
CTTTCTGTCCGTGGGCCTGGTCCTCGTTACCACCCTGGTGTGGCACCTCCTGAGGACACCCCCAGAGCCA
CCCACCCCACTGCCCCCTGAGGACAGGCGCCAGTCAGTGAGCCGCCAGCCCTCCTTCACCTACTCAGAGT
GGATGGAGGAGAAGATCGAGGATGACTTCCTGGACCTCGACCCGGTGCCCGAGACTCCTGTGTTTGATTG
TGTGATGGACATCAAGCCTGAGGCTGACCCCACCTCACTCACCGTCAAGTCCATGGGTCTGCAGGAGAGG
AGGGGTTCCAATGTCTCCCTGACCCTGGACATGTGCACTCCGGGCTGCAACGAGGAGGGCTTTGGCTATC
TCATGTCCCCACGTGAGGAGTCCGCCCGCGAGTACCTGCTCAGCGCCTCCCGTGTCCTCCAAGCAGAAGA
GCTTCATGAAAAGGCCCTGGACCCTTTCCTGCTGCAGGCGGAATTCTTTGAAATCCCCATGAACTTTGTG
GATCCGAAAGAGTACGACATCCCTGGGCTGGTGCGGAAGAACCGGTACAAAACCATACTTCCCAACCCTC
ACAGCAGAGTGTGTCTGACCTCACCAGACCCTGACGACCCTCTGAGTTCCTACATCAATGCCAACTACAT
CCGGGGCTATGGTGGGGAGGAGAAGGTGTACATCGCCACTCAGGGACCCATCGTCAGCACGGTCGCCGAC
TTCTGGCGCATGGTGTGGCAGGAGCACACGCCCATCATTGTCATGATCACCAACATCGAGGAGATGAACG
AGAAATGCACCGAGTATTGGCCGGAGGAGCAGGTGGCGTACGACGGTGTTGAGATCACTGTGCAGAAAGT
CATTCACACGGAGGATTACCGGCTGCGACTCATCTCCCTCAAGAGTGGGACTGAGGAGCGAGGCCTGAAG
CATTACTGGTTCACATCCTGGCCCGACCAGAAGACCCCAGACCGGGCCCCCCCACTCCTGCACCTGGTGC
GGGAGGTGGAGGAGGCAGCCCAGCAGGAGGGGCCCCACTGTGCCCCCATCATCGTCCACTGCAGTGCAGG
GATTGGGAGGACCGGCTGCTTCATTGCCACCAGCATCTGCTGCCAGCAGCTGCGGCAGGAGGGTGTGGTG
GACATCCTGAAGACCACGTGCCAGCTCCGTCAGGACAGGGGCGGCATGATCCAGACATGCGAGCAGTACC
AGTTTGTGCACCACGTCATGAGCCTCTACGAAAAGCAGCTGTCCCACCAGTCCCCAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC217419 representing NM_001039970
Red=Cloning site Green=Tags(s)

MNYEGARSERENHAADDSEGGALDMCCSERLPGLPQPIVMEALDEAEGLQDSQREMPPPPPPSPPSDPAQ
KPPPRGAGSHSLTVRSSLCLFAASQFLLEPTAWLDSGTWGVPSLLLVFLSVGLVLVTTLVWHLLRTPPEP
PTPLPPEDRRQSVSRQPSFTYSEWMEEKIEDDFLDLDPVPETPVFDCVMDIKPEADPTSLTVKSMGLQER
RGSNVSLTLDMCTPGCNEEGFGYLMSPREESAREYLLSASRVLQAEELHEKALDPFLLQAEFFEIPMNFV
DPKEYDIPGLVRKNRYKTILPNPHSRVCLTSPDPDDPLSSYINANYIRGYGGEEKVYIATQGPIVSTVAD
FWRMVWQEHTPIIVMITNIEEMNEKCTEYWPEEQVAYDGVEITVQKVIHTEDYRLRLISLKSGTEERGLK
HYWFTSWPDQKTPDRAPPLLHLVREVEEAAQQEGPHCAPIIVHCSAGIGRTGCFIATSICCQQLRQEGVV
DILKTTCQLRQDRGGMIQTCEQYQFVHHVMSLYEKQLSHQSPE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039970
ORF Size 1599 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039970.2
RefSeq Size 3105 bp
RefSeq ORF 1602 bp
Locus ID 84867
UniProt ID P54829, Q86TL3
Cytogenetics 11p15.1
Protein Families Druggable Genome, Phosphatase, Transmembrane
Protein Pathways MAPK signaling pathway
MW 59.9 kDa
Summary May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001039970" in other vectors (4)
SKU Description Size Price
RC217419L3 Lenti-ORF clone of PTPN5 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) (PTPN5), transcript variant 3 10 ug
$796.00
RC217419L4 Lenti-ORF clone of PTPN5 (mGFP-tagged)-Human protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) (PTPN5), transcript variant 3 10 ug
$796.00
RG217419 PTPN5 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) (PTPN5), transcript variant 3 10 ug
$696.00
SC311095 PTPN5 (untagged)-Human protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) (PTPN5), transcript variant 3 10 ug
$547.00

Plasmid Map for RC217419

Download sequence for

Cloning scheme for RC217419

Circular map for RC217419

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