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PTPN5 (NM_001039970) Human Untagged Clone

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SKU
SC311095
PTPN5 (untagged)-Human protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) (PTPN5), transcript variant 3
  $547.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PTPN5
Synonyms PTPSTEP; STEP; STEP61
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC311095 representing NM_001039970.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAATTATGAGGGAGCCAGGAGTGAGAGAGAGAACCACGCTGCTGATGACTCCGAGGGAGGGGCCCTG
GACATGTGCTGCAGTGAGAGGCTACCGGGTCTCCCCCAGCCGATAGTGATGGAGGCACTGGACGAGGCT
GAAGGGCTCCAGGACTCACAGAGAGAGATGCCGCCACCCCCTCCTCCCTCGCCGCCCTCAGATCCAGCT
CAGAAGCCACCACCTCGAGGCGCTGGGAGCCACTCCCTCACTGTCAGGAGCAGCCTGTGCCTGTTCGCT
GCCTCACAGTTCCTGCTGGAACCCACGGCCTGGCTTGACTCTGGGACGTGGGGAGTCCCCAGTCTGCTG
CTGGTCTTTCTGTCCGTGGGCCTGGTCCTCGTTACCACCCTGGTGTGGCACCTCCTGAGGACACCCCCA
GAGCCACCCACCCCACTGCCCCCTGAGGACAGGCGCCAGTCAGTGAGCCGCCAGCCCTCCTTCACCTAC
TCAGAGTGGATGGAGGAGAAGATCGAGGATGACTTCCTGGACCTCGACCCGGTGCCCGAGACTCCTGTG
TTTGATTGTGTGATGGACATCAAGCCTGAGGCTGACCCCACCTCACTCACCGTCAAGTCCATGGGTCTG
CAGGAGAGGAGGGGTTCCAATGTCTCCCTGACCCTGGACATGTGCACTCCGGGCTGCAACGAGGAGGGC
TTTGGCTATCTCATGTCCCCACGTGAGGAGTCCGCCCGCGAGTACCTGCTCAGCGCCTCCCGTGTCCTC
CAAGCAGAAGAGCTTCATGAAAAGGCCCTGGACCCTTTCCTGCTGCAGGCGGAATTCTTTGAAATCCCC
ATGAACTTTGTGGATCCGAAAGAGTACGACATCCCTGGGCTGGTGCGGAAGAACCGGTACAAAACCATA
CTTCCCAACCCTCACAGCAGAGTGTGTCTGACCTCACCAGACCCTGACGACCCTCTGAGTTCCTACATC
AATGCCAACTACATCCGGGGCTATGGTGGGGAGGAGAAGGTGTACATCGCCACTCAGGGACCCATCGTC
AGCACGGTCGCCGACTTCTGGCGCATGGTGTGGCAGGAGCACACGCCCATCATTGTCATGATCACCAAC
ATCGAGGAGATGAACGAGAAATGCACCGAGTATTGGCCGGAGGAGCAGGTGGCGTACGACGGTGTTGAG
ATCACTGTGCAGAAAGTCATTCACACGGAGGATTACCGGCTGCGACTCATCTCCCTCAAGAGTGGGACT
GAGGAGCGAGGCCTGAAGCATTACTGGTTCACATCCTGGCCCGACCAGAAGACCCCAGACCGGGCCCCC
CCACTCCTGCACCTGGTGCGGGAGGTGGAGGAGGCAGCCCAGCAGGAGGGGCCCCACTGTGCCCCCATC
ATCGTCCACTGCAGTGCAGGGATTGGGAGGACCGGCTGCTTCATTGCCACCAGCATCTGCTGCCAGCAG
CTGCGGCAGGAGGGTGTGGTGGACATCCTGAAGACCACGTGCCAGCTCCGTCAGGACAGGGGCGGCATG
ATCCAGACATGCGAGCAGTACCAGTTTGTGCACCACGTCATGAGCCTCTACGAAAAGCAGCTGTCCCAC
CAGTCCCCAGAATGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001039970
Insert Size 1602 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039970.1
RefSeq Size 3105 bp
RefSeq ORF 1602 bp
Locus ID 84867
UniProt ID P54829
Cytogenetics 11p15.1
Protein Families Druggable Genome, Phosphatase, Transmembrane
Protein Pathways MAPK signaling pathway
MW 60 kDa
Summary May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) has an alternate splice site in the 5' coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment, compared to isoform a.
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Resources
"NM_001039970" in other vectors (4)
SKU Description Size Price
RC217419 PTPN5 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) (PTPN5), transcript variant 3 10 ug
$496.00
RC217419L3 Lenti-ORF clone of PTPN5 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) (PTPN5), transcript variant 3 10 ug
$796.00
RC217419L4 Lenti-ORF clone of PTPN5 (mGFP-tagged)-Human protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) (PTPN5), transcript variant 3 10 ug
$796.00
RG217419 PTPN5 (tGFP-tagged) - Human protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched) (PTPN5), transcript variant 3 10 ug
$696.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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