CNOT4 (NM_013316) Human Tagged ORF Clone
- TrueORF®
SKU
RC217418
CNOT4 (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 1
$892.00
2 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CNOT4 |
| Synonyms | CLONE243; NOT4; NOT4H |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_013316 |
| ORF Size | 1917 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_013316.2, NP_037448.2 |
| RefSeq Size | 3333 bp |
| RefSeq ORF | 1920 bp |
| Locus ID | 4850 |
| UniProt ID | O95628 |
| Cytogenetics | 7q33 |
| Domains | RRM, RRM_1 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | RNA degradation |
| MW | 70.2 kDa |
| Summary | The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.provided by RefSeq, Jul 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_013316" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC217418L1 | Lenti ORF clone of Human CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,192.00
|
|
| RC217418L2 | Lenti ORF clone of Human CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 1, mGFP tagged | 10 ug |
$1,192.00
|
|
| RC217418L3 | Lenti ORF clone of Human CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,192.00
|
|
| RC217418L4 | Lenti ORF clone of Human CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 1, mGFP tagged | 10 ug |
$1,192.00
|
|
| RG217418 | CNOT4 (tGFP-tagged) - Human CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 1 | 10 ug |
$1,092.00
|
|
| SC115278 | CNOT4 (untagged)-Human CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 1 | 10 ug |
$596.00
|
|
Citations
MISTERMINATE Mechanistically Links Mitochondrial Dysfunction with Proteostasis Failure
Wu, Tantray, Lim et al
Mol Cell (2019) 75 (4), 835-848.e8 DOI: 10.1016/j.molcel.2019.06.031
Wu, Tantray, Lim et al
Mol Cell (2019) 75 (4), 835-848.e8 DOI: 10.1016/j.molcel.2019.06.031
Cited by 112
AI Summary: OriGene products, specifically the C-I30 antibody (Cat# TA500053) and the NDUFS3 antibody (Cat# TA501481), were used in this study to detect and analyze the levels and modifications of mitochondrial complex I subunits, thereby helping to elucidate the molecular mechanisms linking mitochondrial dysfunction to proteostasis failure.
MISTERMINATE Mechanistically Links Mitochondrial Dysfunction with Proteostasis Failure
Wu, Tantray, Lim et al
Molecular Cell (2019) 75 (4), 835-848.e8 DOI: 10.1016/j.molcel.2019.06.031
Wu, Tantray, Lim et al
Molecular Cell (2019) 75 (4), 835-848.e8 DOI: 10.1016/j.molcel.2019.06.031
AI Summary: OriGene products pCMV6-FLAG-NOT4 (cat#: RC217418) and pCMV6-ANKZF1 (cat#: RC201054 TrueORF) were used as expression plasmids to investigate the molecular mechanisms linking mitochondrial dysfunction and proteostasis failure in this study.
Snippet: … MISTERMINATE Mechanistically Links Mitochondrial Dysfunction with Proteostasis Failure Zhihao Wu1, Ishaq Tantray1, Junghyun Lim1,2, Songjie Chen3, Yu Li1, Zoe Davis4, Cole Sitron4, Jason Dong1, Suzana Gispert5 View as HTML;pCMV6-FLAG-NOT4 and pCMV6- ANKZF1 was obtained from OriGene Inc (cat#: RC217418 and RC201054 TrueORF). …
Ubiquitination of ABCE1 by NOT4 in Response to Mitochondrial Damage Links Co-translational Quality Control to PINK1-Directed Mitophagy
Wu, Wang, Lim et al
Cell Metab. (2018) 28 (1), 130-144.e7 DOI: 10.1016/j.cmet.2018.05.007
Wu, Wang, Lim et al
Cell Metab. (2018) 28 (1), 130-144.e7 DOI: 10.1016/j.cmet.2018.05.007
AI Summary: OriGene contributed the pCMV-FLAG-NOT4 plasmid (TrueORF, RC217418) to this study, enabling the investigation of NOT4's role in the ubiquitination of ABCE1 and the regulation of mitophagy in response to mitochondrial damage.
Snippet: pMS2-bs, pC-I 30/MS2-bs and pMS2-BP/GST plasmids were described before (Gehrke et al., 2015). pCMV-FLAG-ABCE1 was a gift from Dr. Ramanujan Hegde. pCMV-FLAG-Pelo was generated by cloning Pelo cDNA into the pCMV vector via KpnI-Xba I sites from an original plasmid from Dr. Ramanujan Hegde. pCMV-FLAG-NOT4 was obtained from OriGene Inc (TrueORF, RC217418)
Showing 1-3 of 3 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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