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RNF13 (NM_007282) Human Tagged ORF Clone

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SKU
RC217183
RNF13 (Myc-DDK-tagged)-Human ring finger protein 13 (RNF13), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RNF13
Synonyms DEE73; EIEE73; RZF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217183 representing NM_007282
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTCTCCATAGGGATGCTCATGCTGTCAGCCACACAAGTCTACACCATCTTGACTGTCCAGCTCT
TTGCATTCTTAAACCTACTGCCTGTAGAAGCAGACATTTTAGCATATAACTTTGAAAATGCATCTCAGAC
ATTTGATGACCTCCCTGCAAGATTTGGTTATAGACTTCCAGCTGAAGGTTTAAAGGGTTTTTTGATTAAC
TCAAAACCAGAGAATGCCTGTGAACCCATAGTGCCTCCACCAGTAAAAGACAATTCATCTGGCACTTTCA
TCGTGTTAATTAGAAGACTTGATTGTAATTTTGATATAAAGGTTTTAAATGCACAGAGAGCAGGATACAA
GGCAGCCATAGTTCACAATGTTGATTCTGATGACCTCATTAGCATGGGATCCAACGACATTGAGGTACTA
AAGAAAATTGACATTCCATCTGTCTTTATTGGTGAATCATCAGCTAATTCTCTGAAAGATGAATTCACAT
ATGAAAAAGGGGGCCACCTTATCTTAGTTCCAGAATTTAGTCTTCCTTTGGAATACTACCTAATTCCCTT
CCTTATCATAGTGGGCATCTGTCTCATCTTGATAGTCATTTTCATGATCACAAAATTTGTCCAGGATAGA
CATAGAGCTAGAAGAAACAGACTTCGTAAAGATCAACTTAAGAAACTTCCTGTACATAAATTCAAGAAAG
GAGATGAGTATGATGTATGTGCCATTTGTTTGGATGAGTATGAAGATGGAGACAAACTCAGAATCCTTCC
CTGTTCCCATGCTTATCATTGCAAGTGTGTAGACCCTTGGCTAACTAAAACCAAAAAAACCTGTCCAGTG
TGCAAGCAAAAAGTTGTTCCTTCTCAAGGCGATTCAGACTCTGACACAGACAGTAGTCAAGAAGAAAATG
AAGTGACAGAACATACCCCTTTACTGAGACCTTTAGCTTCTGTCAGTGCCCAGTCATTTGGGGCTTTATC
GGAATCCCGCTCACATCAGAACATGACAGAATCTTCAGACTATGAGGAAGACGACAATGAAGATACTGAC
AGTAGTGATGCAGAAAATGAAATTAATGAACATGATGTCGTGGTCCAGTTGCAGCCTAATGGTGAACGGG
ATTACAACATAGCAAATACTGTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC217183 representing NM_007282
Red=Cloning site Green=Tags(s)

MLLSIGMLMLSATQVYTILTVQLFAFLNLLPVEADILAYNFENASQTFDDLPARFGYRLPAEGLKGFLIN
SKPENACEPIVPPPVKDNSSGTFIVLIRRLDCNFDIKVLNAQRAGYKAAIVHNVDSDDLISMGSNDIEVL
KKIDIPSVFIGESSANSLKDEFTYEKGGHLILVPEFSLPLEYYLIPFLIIVGICLILIVIFMITKFVQDR
HRARRNRLRKDQLKKLPVHKFKKGDEYDVCAICLDEYEDGDKLRILPCSHAYHCKCVDPWLTKTKKTCPV
CKQKVVPSQGDSDSDTDSSQEENEVTEHTPLLRPLASVSAQSFGALSESRSHQNMTESSDYEEDDNEDTD
SSDAENEINEHDVVVQLQPNGERDYNIANTV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007282
ORF Size 1143 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007282.3, NP_009213.1
RefSeq Size 2956 bp
RefSeq ORF 1146 bp
Locus ID 11342
UniProt ID O43567
Cytogenetics 3q25.1
Domains PA, RING
Protein Families Druggable Genome, Protease, Transmembrane
MW 42.6 kDa
Summary The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. The specific function of this gene has not yet been determined. Alternatively spliced transcript variants that encode the same protein have been reported. A pseudogene, which is also located on chromosome 3, has been defined for this gene. provided by RefSeq, Jul 2008
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"NM_007282" in other vectors (6)
SKU Description Size Price
RC217183L1 Lenti ORF clone of Human ring finger protein 13 (RNF13), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC217183L2 Lenti ORF clone of Human ring finger protein 13 (RNF13), transcript variant 1, mGFP tagged 10 ug
$757.00
RC217183L3 Lenti ORF clone of Human ring finger protein 13 (RNF13), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC217183L4 Lenti ORF clone of Human ring finger protein 13 (RNF13), transcript variant 1, mGFP tagged 10 ug
$757.00
RG217183 RNF13 (tGFP-tagged) - Human ring finger protein 13 (RNF13), transcript variant 1 10 ug
$489.00 $657.00
SC111768 RNF13 (untagged)-Human ring finger protein 13 (RNF13), transcript variant 1 10 ug
$457.00

Plasmid Map for RC217183

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC217183
Circular map for RC217183

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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