MET (NM_000245) Human Tagged ORF Clone
SKU
RC217003
MET (Myc-DDK-tagged)-Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2
$1,783.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MET |
| Synonyms | AUTS9; c-Met; DFNB97; HGFR; RCCP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000245 |
| ORF Size | 4170 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000245.4 |
| RefSeq Size | 6641 bp |
| RefSeq ORF | 4173 bp |
| Locus ID | 4233 |
| UniProt ID | P08581, A0A024R759 |
| Cytogenetics | 7q31.2 |
| Protein Families | Druggable Genome, Protein Kinase, Transmembrane |
| Protein Pathways | Adherens junction, Axon guidance, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma |
| MW | 155.54 kDa |
| Summary | This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. provided by RefSeq, May 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000245" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC217003L1 | Lenti ORF clone of Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2, Myc-DDK-tagged | 10 ug |
$2,083.00
|
|
| RC217003L2 | Lenti ORF clone of Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2, mGFP tagged | 10 ug |
$2,083.00
|
|
| RC217003L3 | Lenti ORF clone of Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2, Myc-DDK-tagged | 10 ug |
$2,083.00
|
|
| RC217003L4 | Lenti ORF clone of Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2, mGFP tagged | 10 ug |
$2,083.00
|
|
| RC600030 | MET (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens met proto-oncogene (hepatocyte growth factor receptor), transcript variant 2, Signal peptide (1-24) plus EC domain (25-932) | 10 ug |
$589.00
$853.00
|
|
| RG217003 | MET (tGFP-tagged) - Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2 | 10 ug |
$1,983.00
|
|
| SC316318 | MET (untagged)-Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2 | 10 ug |
$1,784.00
|
|
Citations
Licochalcone A Promotes the Ubiquitination of c-Met to Abrogate Gefitinib Resistance
Han, Li, Gan et al
Biomed Res Int (2022) 2022, 5687832 DOI: 10.1155/2022/5687832
Han, Li, Gan et al
Biomed Res Int (2022) 2022, 5687832 DOI: 10.1155/2022/5687832
Cited by 18
AI Summary: OriGene products, specifically the c-Met (Cat# TA503367) and c-Cbl (Cat# TA500411) antibodies, were used in this study to detect and analyze the expression and ubiquitination of c-Met and c-Cbl proteins, thereby supporting the investigation of Licochalcone A’s mechanism in overcoming gefitinib resistance in NSCLC cells.
MET Mutation Is a Potential Therapeutic Target for Advanced Endometrial Cancer
Yeh, Wu, Lin et al
Cancers (Basel) (2021) 13 (16) DOI: 10.3390/cancers13164231
Yeh, Wu, Lin et al
Cancers (Basel) (2021) 13 (16) DOI: 10.3390/cancers13164231
Cited by 6
AI Summary: OriGene products, specifically the c-MET expression plasmid (RC210447) and c-MET shRNA (TG311849), were used in this study to manipulate MET gene expression in cell lines, enabling the authors to investigate the functional impact of MET mutations on tumor growth, drug sensitivity, and signaling in advanced endometrial cancer.
Met Mutation is a Potential Therapeutic Target for Advanced Endometrial Cancer
Yeh, Wu, Lin et al
Research Square (2020)
Yeh, Wu, Lin et al
Research Square (2020)
Mutant ACTB mRNA 3'-UTR promotes hepatocellular carcinoma development by regulating miR-1 and miR-29a
Li, Ma, Shi et al
Cell. Signal. (2019) 67, 109479 DOI: 10.1016/j.cellsig.2019.109479
Li, Ma, Shi et al
Cell. Signal. (2019) 67, 109479 DOI: 10.1016/j.cellsig.2019.109479
Cited by 21
AI Summary: In this study, OriGene’s MCL1 (RC200521) and MET (RC217003) plasmids were used to investigate how mutant ACTB mRNA 3'-UTR promotes hepatocellular carcinoma by regulating the expression of these miR-1 and miR-29a target genes.
Snippet: A full-length fragment of the ACTB 3’-UTR wild type or mutant was amplified. The PCR product was cloned into the pGL3-promoter luciferase-based plasmid (Promega, Madison, WI, USA) at the cloning site between KpnI and XhoI. The amplified fragment was verified by DNA sequencing. The plasmids MCL1 (RC200521) and MET (RC217003) were purchased from Origene
Deguelin suppresses angiogenesis in human hepatocellular carcinoma by targeting HGF-c-Met pathway
Li, Yu, Li et al
Oncotarget (2018) 9 (1), 152-166 DOI: 10.18632/oncotarget.22077
Li, Yu, Li et al
Oncotarget (2018) 9 (1), 152-166 DOI: 10.18632/oncotarget.22077
Snippet: TRCN0000000422, TRCN0000000426) were purchased from GE Dharmacon. The c-Met overexpression plasmid (RC217003) was purchased from OriGene Technologies, Inc. (Rockville, MD, USA). The pLKO.1-shGFP (Addgene plasmid
Showing 1-5 of 5 papers.
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