MET (NM_000245) Human Tagged Lenti ORF Clone

SKU
RC217003L1
Lenti ORF clone of Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2, Myc-DDK-tagged
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

    Click here to learn more.

$2,083.00
In Stock*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MET
Synonyms AUTS9; c-Met; DFNB97; HGFR; RCCP2
Vector pLenti-C-Myc-DDK
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC217003).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_000245
ORF Size 4170 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000245.2
RefSeq Size 6641 bp
RefSeq ORF 4173 bp
Locus ID 4233
UniProt ID P08581
Cytogenetics 7q31.2
Protein Families Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways Adherens junction, Axon guidance, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma
MW 155.54 kDa
Summary This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]
Write Your Own Review
You're reviewing:MET (NM_000245) Human Tagged Lenti ORF Clone
Your Rating
SKU Description Size Price
RC217003 MET (Myc-DDK-tagged)-Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2 10 ug
$1,783.00
RC217003L2 Lenti ORF clone of Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2, mGFP tagged 10 ug
$2,083.00
RC217003L3 Lenti ORF clone of Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2, Myc-DDK-tagged 10 ug
$2,083.00
RC217003L4 Lenti ORF clone of Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2, mGFP tagged 10 ug
$2,083.00
RC600030 MET (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens met proto-oncogene (hepatocyte growth factor receptor), transcript variant 2, Signal peptide (1-24) plus EC domain (25-932) 10 ug
$589.00 MSRP $853.00 MSRP $853.00
RG217003 MET (tGFP-tagged) - Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2 10 ug
$1,983.00
SC316318 MET (untagged)-Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2 10 ug
$1,784.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.