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WAVE 1 (WASF1) (NM_003931) Human Tagged ORF Clone

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SKU
RC216977
WASF1 (Myc-DDK-tagged)-Human WAS protein family, member 1 (WASF1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $520.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol WAVE 1
Synonyms NEDALVS; SCAR1; WAVE; WAVE1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216977 representing NM_003931
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCTAGTGAAAAGAAACATCGATCCTAGGCACTTGTGCCACACAGCACTGCCTAGAGGCATTAAGA
ATGAACTGGAATGTGTAACCAATATTTCCTTGGCAAATATAATTAGACAACTAAGTAGCCTAAGTAAATA
TGCTGAAGATATATTTGGAGAATTATTCAATGAAGCACATAGTTTTTCCTTCAGAGTCAACTCATTGCAA
GAACGTGTGGACCGTTTATCTGTTAGTGTTACACAGCTTGATCCAAAGGAAGAAGAATTGTCTTTGCAAG
ATATAACAATGAGGAAAGCTTTCCGAAGTTCTACAATTCAAGACCAGCAGCTTTTCGATCGCAAGACTTT
GCCTATTCCATTACAGGAGACGTACGATGTTTGTGAACAGCCTCCACCTCTCAATATACTCACTCCTTAT
AGAGATGATGGTAAAGAAGGTCTGAAGTTTTATACCAATCCTTCGTATTTCTTTGATCTATGGAAAGAAA
AAATGTTGCAAGATACAGAGGATAAGAGGAAGGAAAAGAGGAAGCAGAAGCAGAAAAATCTAGATCGTCC
TCATGAACCAGAAAAAGTGCCAAGAGCACCTCATGACAGGCGGCGAGAATGGCAGAAGCTGGCCCAAGGT
CCAGAGCTGGCTGAAGATGATGCTAATCTCTTACATAAGCATATTGAAGTTGCTAATGGCCCAGCCTCTC
ATTTTGAAACAAGACCTCAGACATACGTGGATCATATGGATGGATCTTACTCACTTTCTGCCTTGCCATT
TAGTCAGATGAGTGAGCTTCTGACTAGAGCTGAGGAAAGGGTATTAGTCAGACCACATGAACCACCTCCA
CCTCCACCAATGCATGGAGCAGGAGATGCAAAACCGATACCCACCTGTATCAGTTCTGCTACAGGTTTGA
TAGAAAATCGCCCTCAGTCACCAGCTACAGGCAGAACACCTGTGTTTGTGAGCCCCACTCCCCCACCTCC
TCCACCACCTCTTCCATCTGCCTTGTCAACTTCCTCATTAAGAGCTTCAATGACTTCAACTCCTCCCCCT
CCAGTACCTCCCCCACCTCCACCTCCAGCCACTGCTTTGCAAGCTCCAGCAGTACCACCACCTCCAGCTC
CTCTTCAGATTGCCCCTGGAGTTCTTCACCCAGCTCCTCCTCCAATTGCACCTCCTCTAGTACAGCCCTC
TCCACCAGTAGCTAGAGCTGCCCCAGTATGTGAGACTGTACCAGTTCATCCACTCCCACAAGGTGAAGTT
CAGGGGCTGCCTCCACCCCCACCACCGCCTCCTCTGCCTCCACCTGGCATTCGACCATCATCACCTGTCA
CAGTTACAGCTCTTGCTCATCCTCCCTCTGGGCTACATCCAACTCCATCTACTGCCCCAGGTCCCCATGT
TCCATTAATGCCTCCATCTCCTCCATCACAAGTTATACCTGCTTCTGAGCCAAAGCGCCATCCATCAACC
CTACCTGTAATCAGTGATGCCAGGAGTGTGCTACTGGAAGCAATACGAAAAGGTATTCAGCTACGCAAAG
TAGAAGAGCAGCGTGAACAGGAAGCTAAGCATGAACGCATTGAAAACGATGTTGCCACCATCCTGTCTCG
CCGTATTGCTGTTGAATATAGTGATTCGGAAGATGATTCAGAATTTGATGAAGTAGATTGGTTGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216977 representing NM_003931
Red=Cloning site Green=Tags(s)

MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQ
ERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNILTPY
RDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQG
PELAEDDANLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLTRAEERVLVRPHEPPP
PPPMHGAGDAKPIPTCISSATGLIENRPQSPATGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPP
PVPPPPPPPATALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEV
QGLPPPPPPPPLPPPGIRPSSPVTVTALAHPPSGLHPTPSTAPGPHVPLMPPSPPSQVIPASEPKRHPST
LPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003931
ORF Size 1677 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003931.3
RefSeq Size 3230 bp
RefSeq ORF 1680 bp
Locus ID 8936
UniProt ID Q92558
Cytogenetics 6q21
Domains WH2
Protein Families Druggable Genome
Protein Pathways Adherens junction, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
MW 61.5 kDa
Summary The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Jul 2008
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Documents
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"NM_003931" in other vectors (6)
SKU Description Size Price
RC216977L1 Lenti ORF clone of Human WAS protein family, member 1 (WASF1), transcript variant 1, Myc-DDK-tagged 10 ug
$820.00
RC216977L2 Lenti ORF clone of Human WAS protein family, member 1 (WASF1), transcript variant 1, mGFP tagged 10 ug
$820.00
RC216977L3 Lenti ORF clone of Human WAS protein family, member 1 (WASF1), transcript variant 1, Myc-DDK-tagged 10 ug
$820.00
RC216977L4 Lenti ORF clone of Human WAS protein family, member 1 (WASF1), transcript variant 1, mGFP tagged 10 ug
$820.00
RG216977 WASF1 (tGFP-tagged) - Human WAS protein family, member 1 (WASF1), transcript variant 1 10 ug
$489.00 $720.00
SC117699 WASF1 (untagged)-Human WAS protein family, member 1 (WASF1), transcript variant 1 10 ug
$521.00

Plasmid Map for RC216977

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216977
Circular map for RC216977

file-alt Citations

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