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DPP6 (NM_001936) Human Tagged ORF Clone

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SKU
RC216875
DPP6 (Myc-DDK-tagged)-Human dipeptidyl-peptidase 6 (DPP6), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $735.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DPP6
Synonyms DPL1; DPPX; MRD33; VF2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216875 representing NM_001936
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCACAGCCAAGGAGCCAAGCGCTTCGGGGAAATCCGTGCAGCAGCAGGAACAGGAGCTGGTGGGGA
GTAACCCTCCGCAGAGGAATTGGAAAGGAATAGCAATTGCACTGCTTGTCATTCTGGTCATCTGCTCCTT
GATCGTCACCTCGGTCATACTTCTGACACCAGCGGAAGATAATAGTCTGTCTCAAAAGAAGAAGGTCACT
GTAGAAGATCTCTTCAGTGAAGACTTCAAAATTCATGACCCCGAGGCTAAGTGGATAAGTGATACAGAAT
TCATCTACAGAGAACAGAAAGGAACAGTGAGACTGTGGAATGTTGAAACAAATACTTCTACTGTCTTAAT
AGAAGGCAAAAAAATTGAATCATTAAGAGCCATCAGATATGAAATATCTCCAGATAGAGAGTATGCACTT
TTTTCATACAATGTGGAACCCATATATCAACACTCGTATACTGGATATTATGTCCTGAGCAAAATTCCTC
ATGGGGATCCTCAAAGTCTGGACCCACCAGAAGTCAGCAATGCAAAGCTTCAGTATGCAGGATGGGGCCC
TAAAGGCCAACAGCTGATATTTATTTTTGAAAACAATATCTACTACTGTGCACATGTCGGGAAACAGGCC
ATCCGTGTGGTCTCCACTGGCAAGGAAGGTGTGATTTACAATGGCCTCAGTGACTGGCTGTATGAAGAGG
AGATTTTGAAGACACACATCGCACACTGGTGGTCTCCGGATGGCACGAGACTCGCCTACGCCGCCATCAA
TGATTCCCGTGTCCCCATCATGGAGCTCCCAACTTACACCGGCTCCATCTACCCCACCGTGAAGCCCTAC
CACTATCCCAAGGCTGGAAGTGAGAACCCCAGCATTTCCCTACACGTTATTGGCTTAAATGGACCCACCC
ATGATCTGGAGATGATGCCGCCTGATGATCCACGGATGAGGGAGTACTACATCACCATGGTGAAGTGGGC
CACCAGCACCAAGGTCGCCGTGACCTGGCTGAACCGGGCGCAGAACGTGTCCATCCTCACCCTCTGCGAC
GCCACCACGGGGGTCTGCACGAAGAAACACGAGGATGAAAGTGAGGCCTGGCTCCACAGACAGAATGAAG
AACCTGTGTTCTCCAAGGATGGCCGAAAGTTTTTCTTCATCAGAGCCATCCCCCAGGGAGGACGAGGGAA
ATTCTATCACATCACGGTGTCCTCGTCCCAGCCCAACAGCAGCAACGACAACATCCAGTCCATCACCTCC
GGGGACTGGGACGTGACCAAGATCCTAGCCTACGATGAGAAGGGGAATAAGATCTACTTCCTGAGCACGG
AGGACCTGCCTCGGAGACGACAACTCTACAGTGCCAACACGGTGGGCAACTTCAACAGGCAGTGCCTCTC
CTGTGACCTGGTTGAGAACTGCACCTACTTCAGCGCTTCCTTCAGCCATAGCATGGACTTCTTCCTGCTC
AAGTGCGAAGGTCCTGGTGTTCCTATGGTGACGGTGCACAACACAACAGATAAGAAAAAAATGTTTGACC
TAGAAACAAATGAACATGTCAAGAAGGCCATAAATGACCGACAGATGCCTAAAGTGGAATACAGGGACAT
TGAGATTGATGATTACAACCTGCCCATGCAGATACTGAAGCCAGCAACCTTCACCGACACCACCCACTAC
CCTCTGCTCCTGGTGGTGGATGGCACCCCGGGCAGCCAGAGTGTGGCTGAGAAGTTCGAGGTGAGCTGGG
AGACGGTGATGGTGAGCAGCCACGGCGCGGTGGTGGTAAAGTGTGACGGCCGTGGCAGCGGCTTCCAAGG
GACCAAGCTCCTGCACGAAGTGAGGCGGCGGCTGGGCTTGCTGGAGGAGAAGGACCAGATGGAGGCCGTG
CGGACGATGCTGAAGGAGCAGTACATTGACAGGACGCGCGTGGCCGTGTTTGGGAAGGATTACGGTGGCT
ACCTGAGCACCTACATCCTCCCAGCAAAGGGAGAAAATCAAGGCCAGACATTCACCTGCGGCTCTGCTCT
CTCTCCAATAACAGACTTCAAACTCTATGCCTCTGCGTTTTCCGAGAGGTACTTGGGCCTCCATGGACTT
GACAACAGAGCATACGAGATGACCAAGGTAGCCCATCGAGTCTCCGCGCTGGAAGAACAGCAGTTCCTGA
TCATTCATCCCACTGCCGATGAAAAAATTCATTTCCAGCACACAGCAGAACTCATTACACAACTAATTAG
GGGAAAGGCTAATTACAGCTTACAGATTTACCCGGACGAAAGCCATTACTTTACCAGCTCCAGCCTCAAA
CAGCATCTGTACCGGTCCATCATCAACTTCTTCGTGGAATGCTTCAGGATCCAGGACAAACTGCTGACAG
TCACAGCGAAAGAGGACGAGGAGGAGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216875 representing NM_001936
Red=Cloning site Green=Tags(s)

MTTAKEPSASGKSVQQQEQELVGSNPPQRNWKGIAIALLVILVICSLIVTSVILLTPAEDNSLSQKKKVT
VEDLFSEDFKIHDPEAKWISDTEFIYREQKGTVRLWNVETNTSTVLIEGKKIESLRAIRYEISPDREYAL
FSYNVEPIYQHSYTGYYVLSKIPHGDPQSLDPPEVSNAKLQYAGWGPKGQQLIFIFENNIYYCAHVGKQA
IRVVSTGKEGVIYNGLSDWLYEEEILKTHIAHWWSPDGTRLAYAAINDSRVPIMELPTYTGSIYPTVKPY
HYPKAGSENPSISLHVIGLNGPTHDLEMMPPDDPRMREYYITMVKWATSTKVAVTWLNRAQNVSILTLCD
ATTGVCTKKHEDESEAWLHRQNEEPVFSKDGRKFFFIRAIPQGGRGKFYHITVSSSQPNSSNDNIQSITS
GDWDVTKILAYDEKGNKIYFLSTEDLPRRRQLYSANTVGNFNRQCLSCDLVENCTYFSASFSHSMDFFLL
KCEGPGVPMVTVHNTTDKKKMFDLETNEHVKKAINDRQMPKVEYRDIEIDDYNLPMQILKPATFTDTTHY
PLLLVVDGTPGSQSVAEKFEVSWETVMVSSHGAVVVKCDGRGSGFQGTKLLHEVRRRLGLLEEKDQMEAV
RTMLKEQYIDRTRVAVFGKDYGGYLSTYILPAKGENQGQTFTCGSALSPITDFKLYASAFSERYLGLHGL
DNRAYEMTKVAHRVSALEEQQFLIIHPTADEKIHFQHTAELITQLIRGKANYSLQIYPDESHYFTSSSLK
QHLYRSIINFFVECFRIQDKLLTVTAKEDEEED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001936
ORF Size 2409 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001936.5
RefSeq Size 3560 bp
RefSeq ORF 2412 bp
Locus ID 1804
UniProt ID A7E2E4
Cytogenetics 7q36.2
Domains DPPIV_N_term, Peptidase_S9
Protein Families Druggable Genome, Protease, Transmembrane
MW 91.2 kDa
Summary This gene encodes a single-pass type II membrane protein that is a member of the peptidase S9B family of serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Variations in this gene may be associated with susceptibility to amyotrophic lateral sclerosis and with idiopathic ventricular fibrillation. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2014
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"NM_001936" in other vectors (4)
SKU Description Size Price
RC216875L3 Lenti ORF clone of Human dipeptidyl-peptidase 6 (DPP6), transcript variant 2, Myc-DDK-tagged 10 ug
$1,035.00
RC216875L4 Lenti ORF clone of Human dipeptidyl-peptidase 6 (DPP6), transcript variant 2, mGFP tagged 10 ug
$1,035.00
RG216875 DPP6 (tGFP-tagged) - Human dipeptidyl-peptidase 6 (DPP6), transcript variant 2 10 ug
$935.00
SC109170 DPP6 (untagged)-Human dipeptidyl-peptidase 6 (DPP6), transcript variant 2 10 ug
$736.00

Plasmid Map for RC216875

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216875
Circular map for RC216875

file-alt Citations

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