SEC31A (NM_001077208) Human Tagged ORF Clone
SKU
RC216802
SEC31A (Myc-DDK-tagged)-Human SEC31 homolog A (S. cerevisiae) (SEC31A), transcript variant 3
$1,573.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SEC31A |
| Synonyms | ABP125; ABP130; HSPC275; HSPC334; NEDSOSB; SEC31L1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001077208 |
| ORF Size | 3615 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001077208.3, NP_001070676.1 |
| RefSeq Size | 4224 bp |
| RefSeq ORF | 3618 bp |
| Locus ID | 22872 |
| UniProt ID | O94979 |
| Cytogenetics | 4q21.22 |
| MW | 131.5 kDa |
| Summary | The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Dec 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001077208" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216802L3 | Lenti ORF clone of Human SEC31 homolog A (S. cerevisiae) (SEC31A), transcript variant 3, Myc-DDK-tagged | 10 ug |
$1,873.00
|
|
| RC216802L4 | Lenti ORF clone of Human SEC31 homolog A (S. cerevisiae) (SEC31A), transcript variant 3, mGFP tagged | 10 ug |
$1,873.00
|
|
| RG216802 | SEC31A (tGFP-tagged) - Human SEC31 homolog A (S. cerevisiae) (SEC31A), transcript variant 3 | 10 ug |
$1,773.00
|
|
| SC315648 | SEC31A (untagged)-Human SEC31 homolog A (S. cerevisiae) (SEC31A), transcript variant 3 | 10 ug |
$1,679.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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