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SLC23A2 (NM_005116) Human Tagged ORF Clone

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SKU
RC216776
SLC23A2 (Myc-DDK-tagged)-Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $907.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC23A2
Synonyms NBTL1; SLC23A1; SVCT2; YSPL2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216776 representing NM_005116.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGATGGGTATTGGTAAGAATACCACATCCAAATCAATGGAGGCTGGAAGTTCAACAGAAGGCAAATAC
GAAGACGAGGCAAAGCACCCAGCTTTCTTCACTCTTCCGGTGGTGATAAATGGAGGCGCCACCTCCAGC
GGTGAGCAGGACAATGAGGACACTGAGCTCATGGCGATCTACACTACGGAAAACGGCATTGCAGAAAAG
AGCTCTCTCGCTGAGACCCTGGATAGCACTGGCAGTCTGGACCCCCAGCGATCAGACATGATTTATACC
ATAGAAGATGTTCCTCCCTGGTACCTGTGTATATTTCTGGGGCTACAGCACTACCTGACATGCTTCAGC
GGCACGATCGCAGTGCCCTTCCTGTTGGCTGATGCCATGTGTGTGGGGTACGACCAGTGGGCCACCAGC
CAGCTCATTGGGACCATTTTCTTCTGTGTGGGAATCACTACTTTGCTACAGACAACGTTTGGATGCAGG
TTACCCCTGTTTCAGGCCAGTGCTTTTGCATTTTTGGCCCCTGCTCGAGCCATCCTGTCTTTAGATAAA
TGGAAATGTAACACCACAGATGTTTCAGTTGCCAATGGAACAGCAGAGCTGTTGCACACAGAACACATC
TGGTATCCCCGGATCCGAGAGATCCAGGGGGCCATCATCATGTCCTCACTGATAGAAGTAGTCATCGGC
CTCCTCGGCCTGCCTGGGGCTCTACTGAAGTACATCGGTCCCTTGACCATTACACCCACGGTGGCCCTA
ATTGGCCTCTCTGGTTTCCAGGCAGCGGGGGAGAGAGCCGGGAAGCACTGGGGCATTGCCATGCTGACA
ATATTCCTAGTATTACTGTTTTCTCAATACGCCAGAAATGTTAAATTTCCTCTCCCGATTTATAAATCC
AAGAAAGGATGGACTGCGTACAAGTTACAGCTGTTCAAAATGTTCCCTATCATCCTGGCCATCCTGGTA
TCCTGGCTGCTCTGCTTCATCTTCACGGTGACAGATGTCTTCCCTCCCGACAGCACAAAGTATGGCTTC
TATGCTCGCACAGATGCCAGGCAAGGCGTGCTTCTGGTAGCCCCGTGGTTTAAGGTTCCATACCCATTT
CAGTGGGGACTGCCCACCGTGTCTGCGGCCGGTGTCATCGGCATGCTCAGTGCCGTGGTCGCCAGCATC
ATCGAGTCTATTGGTGACTACTACGCCTGTGCACGGCTGTCCTGTGCCCCACCCCCCCCCATCCACGCA
ATAAACAGGGGAATTTTCGTGGAAGGCCTCTCCTGTGTTCTTGATGGCATATTTGGTACTGGGAATGGC
TCTACTTCATCCAGTCCCAACATTGGAGTTTTGGGAATTACAAAGGTCGGCAGCCGCCGCGTGATACAG
TGCGGAGCAGCCCTCATGCTCGCTCTGGGCATGATCGGGAAGTTCAGCGCCCTCTTTGCGTCCCTTCCG
GATCCTGTGCTGGGAGCCCTGTTCTGCACGCTCTTTGGAATGATCACAGCTGTTGGCCTCTCTAACCTG
CAGTTCATTGATTTAAATTCTTCCCGGAACCTCTTTGTGCTTGGATTTTCGATCTTCTTTGGGCTCGTC
CTTCCAAGTTACCTCAGACAGAACCCTCTGGTCACAGGGATAACAGGAATCGATCAAGTGTTGAACGTC
CTTCTCACAACTGCTATGTTTGTAGGGGGCTGTGTGGCTTTTATCCTGGATAACACCATCCCAGGCACT
CCAGAGGAAAGAGGAATCCGGAAATGGAAGAAGGGTGTGGGCAAAGGGAACAAATCACTCGACGGCATG
GAGTCGTACAATTTGCCATTTGGCATGAACATTATAAAAAAATACAGATGCTTCAGCTACTTACCCATC
AGCCCAACCTTTGTGGGCTACACATGGAAAGGCCTCAGGAAGAGCGACAACAGCCGGAGTTCAGATGAA
GACTCCCAGGCCACGGGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Protein Sequence
>Peptide sequence encoded by RC216776
Blue=ORF Red=Cloning site Green=Tag(s)

MMGIGKNTTSKSMEAGSSTEGKYEDEAKHPAFFTLPVVINGGATSSGEQDNEDTELMAIYTTENGIAEK
SSLAETLDSTGSLDPQRSDMIYTIEDVPPWYLCIFLGLQHYLTCFSGTIAVPFLLADAMCVGYDQWATS
QLIGTIFFCVGITTLLQTTFGCRLPLFQASAFAFLAPARAILSLDKWKCNTTDVSVANGTAELLHTEHI
WYPRIREIQGAIIMSSLIEVVIGLLGLPGALLKYIGPLTITPTVALIGLSGFQAAGERAGKHWGIAMLT
IFLVLLFSQYARNVKFPLPIYKSKKGWTAYKLQLFKMFPIILAILVSWLLCFIFTVTDVFPPDSTKYGF
YARTDARQGVLLVAPWFKVPYPFQWGLPTVSAAGVIGMLSAVVASIIESIGDYYACARLSCAPPPPIHA
INRGIFVEGLSCVLDGIFGTGNGSTSSSPNIGVLGITKVGSRRVIQCGAALMLALGMIGKFSALFASLP
DPVLGALFCTLFGMITAVGLSNLQFIDLNSSRNLFVLGFSIFFGLVLPSYLRQNPLVTGITGIDQVLNV
LLTTAMFVGGCVAFILDNTIPGTPEERGIRKWKKGVGKGNKSLDGMESYNLPFGMNIIKKYRCFSYLPI
SPTFVGYTWKGLRKSDNSRSSDEDSQATG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005116
ORF Size 1950 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005116.4
RefSeq Size 6953 bp
RefSeq ORF 1953 bp
Locus ID 9962
UniProt ID Q9UGH3, A0A140VK48
Cytogenetics 20p13
Domains xan_ur_permease
Protein Families Transmembrane
MW 70.3 kDa
Summary The absorption of vitamin C into the body and its distribution to organs requires two sodium-dependent vitamin C transporters. This gene encodes one of the two required transporters and the encoded protein accounts for tissue-specific uptake of vitamin C. Previously, this gene had an official symbol of SLC23A1. provided by RefSeq, Jul 2008
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"NM_005116" in other vectors (6)
SKU Description Size Price
RC216776L1 Lenti ORF clone of Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1, Myc-DDK-tagged 10 ug
$1,207.00
RC216776L2 Lenti ORF clone of Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1, mGFP tagged 10 ug
$1,207.00
RC216776L3 Lenti ORF clone of Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1, Myc-DDK-tagged 10 ug
$1,207.00
RC216776L4 Lenti ORF clone of Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1, mGFP tagged 10 ug
$1,207.00
RG216776 SLC23A2 (tGFP-tagged) - Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1 10 ug
$1,107.00
SC116937 SLC23A2 (untagged)-Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1 10 ug
$606.00

Plasmid Map for RC216776

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216776
Circular map for RC216776

file-alt Citations

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