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SLC23A2 (NM_005116) Human Untagged Clone

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SKU
SC116937
SLC23A2 (untagged)-Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1
  $606.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SLC23A2
Synonyms NBTL1; SLC23A1; SVCT2; YSPL2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC116937 sequence for NM_005116 edited (data generated by NextGen Sequencing)
ATGATGGGTATTGGTAAGAATACCACATCCAAATCAATGGAGGCTGGAAGTTCAACAGAA
GGCAAATACGAAGACGAGGCAAAGCACCCAGCTTTCTTCACTCTTCCGGTGGTGATAAAT
GGAGGCGCCACCTCCAGCGGTGAGCAGGACAATGAGGACACTGAGCTCATGGCGATCTAC
ACTACGGAAAACGGCATTGCAGAAAAGAGCTCTCTCGCTGAGACCCTGGATAGCACTGGC
AGTCTGGACCCCCAGCGATCAGACATGATTTATACCATAGAAGATGTTCCTCCCTGGTAC
CTGTGTATATTTCTGGGGCTACAGCACTACCTGACATGCTTCAGCGGCACGATCGCAGTG
CCCTTCCTGTTGGCTGATGCCATGTGTGTGGGGTACGACCAGTGGGCCACCAGCCAGCTC
ATTGGGACCATTTTCTTCTGTGTGGGAATCACTACTTTGCTACAGACAACGTTTGGATGC
AGGTTACCCCTGTTTCAGGCCAGTGCTTTTGCATTTTTGGCCCCTGCTCGAGCCATCCTG
TCTTTAGATAAATGGAAATGTAACACCACAGATGTTTCAGTTGCCAATGGAACAGCAGAG
CTGTTGCACACAGAACACATCTGGTATCCCCGGATCCGAGAGATCCAGGGGGCCATCATC
ATGTCCTCACTGATAGAAGTAGTCATCGGCCTCCTCGGCCTGCCTGGGGCTCTACTGAAG
TACATCGGTCCCTTGACCATTACACCCACGGTGGCCCTAATTGGCCTCTCTGGTTTCCAG
GCAGCGGGGGAGAGAGCCGGGAAGCACTGGGGCATTGCCATGCTGACAATATTCCTAGTA
TTACTGTTTTCTCAATACGCCAGAAATGTTAAATTTCCTCTCCCGATTTATAAATCCAAG
AAAGGATGGACTGCGTACAAGTTACAGCTGTTCAAAATGTTCCCTATCATCCTGGCCATC
CTGGTATCCTGGCTGCTCTGCTTCATCTTCACGGTGACAGATGTCTTCCCTCCCGACAGC
ACAAAGTATGGCTTCTATGCTCGCACAGATGCCAGGCAAGGCGTGCTTCTGGTAGCCCCG
TGGTTTAAGGTTCCATACCCATTTCAGTGGGGACTGCCCACCGTGTCTGCGGCCGGTGTC
ATCGGCATGCTCAGTGCCGTGGTCGCCAGCATCATCGAGTCTATTGGTGACTACTACGCC
TGTGCACGGCTGTCCTGTGCCCCACCCCCCCCCATCCACGCAATAAACAGGGGAATTTTC
GTGGAAGGCCTCTCCTGTGTTCTTGATGGCATATTTGGTACTGGGAATGGCTCTACTTCA
TCCAGTCCCAACATTGGAGTTTTGGGAATTACAAAGGTCGGCAGCCGCCGCGTGATACAG
TGCGGAGCAGCCCTCATGCTCGCTCTGGGCATGATCGGGAAGTTCAGCGCCCTCTTTGCG
TCCCTTCCGGATCCTGTGCTGGGAGCCCTGTTCTGCACGCTCTTTGGAATGATCACAGCT
GTTGGCCTCTCTAACCTGCAGTTCATTGATTTAAATTCTTCCCGGAACCTCTTTGTGCTT
GGATTTTCGATCTTCTTTGGGCTCGTCCTTCCAAGTTACCTCAGACAGAACCCTCTGGTC
ACAGGGATAACAGGAATCGATCAAGTGTTGAACGTCCTTCTCACAACTGCTATGTTTGTA
GGGGGCTGTGTGGCTTTTATCCTGGATAACACCATCCCAGGCACTCCAGAGGAAAGAGGA
ATCCGGAAATGGAAGAAGGGTGTGGGCAAAGGGAACAAATCACTCGACGGCATGGAGTCG
TACAATTTGCCATTTGGCATGAACATTATAAAAAAATACAGATGCTTCAGCTACTTACCC
ATCAGCCCAACCTTTGTGGGCTACACATGGAAAGGCCTCAGGAAGAGCGACAACAGCCGG
AGTTCAGATGAAGACTCCCAGGCCACGGGATAG

Clone variation with respect to NM_005116.5
375 c=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005116 unedited
TGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCGGAGGCGGAGGCTGC
AGCGGGCACTGCGGCGCCGAGGCGCGGCGGCAGGACGGGAACCACGCGAGCTGCAGCCAG
GTCTTGACTCCACTAACGGGGACTTCTCATACGGCTTTGAACTTGGACCTTGGTGCATAA
GCAAATGTAACTCAGCGAATTGTAATGGAAAGCATATGGACAGGTCTACGCCACATCACT
TAGGTGCTAAGAGATGCCGTTTGCAGGCCTCATCTCTGCTTGAGAATCGGGGGCTCCAGG
CACTAGAAGAGCCGGCTGATCCTGGGCTCCTAGCTTGAATAAGCCTTCACTTCCAGCTGC
TCTCCCCAACGGCTGTGTAAACTACTCGTTTCTCTTAATGATGGGTATTGGTAAGAATAC
CACATCCAAATCAATGGAGGCTGGAAGTTCAACAGAAGGCAAATACGAAGACGAGGCAAA
GCACCCAGCTTTCTTCACTCTTCCGGTGGTGATAAATGGAGGCGCCACCTCCAGCGGTGA
GCAGGACAATGAGGACACTGAGCTCATGGCGATCTACACTACGGAAAACGGCATTGCAGA
AAAGAGCTCTCTCGCTGAGACCCTGGATAGCACTGGCAGTCTGGACCCCCAGCGATCAGA
CATGATTTATACCATAGAAGATGTTCCTCCCTGGTACCTGTGTATATTTCTGGGGCTACA
GCACTACCTGACATGCTTCAGCGGCACGATCGCAGTGCCCTTCCTGTTGGCTGATGCCAT
GTGTGTGGGGTACGACCAGTGGGCCACCAGCCAGCTCATTGGGACCATTTTCTTCTGTGT
GGGAATCACTACNTTGCTACAGACCACGTTGGATGCANGTTACCCCTGNTTCANGCCCAG
TGCTTTGCATTNTTGGNCCCTGCTCG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005116 unedited
NAAATACTTGNACCGCGCCGCTTTCTANGATCGAGTTTTTTTTTTTTTTTTTTGTGTCCA
GGAGACTTTTATTTGTACGGAACAGTTTTAAAAATACAGAATAAAATAGCTTTATCTCTA
CTATTATTCACAACATCTGGTCCAAATATTACATATTTTTAAAATACTTAAACGTTTAAT
GAAGCCATGTTAGAAAAACAATATGAAAATTCTTTTTAAAAACTCCATAAAAGAATTACA
CATCTCAAAAAACAACCCCACCCCAAATACCCTCGGTAATCAAAATAAAAATAAAAAACA
AACAAAACCCCAGAAAGGTATATATAGCCAAAGTCAGTCACAAAAACTCAAGGAAAATAT
AGGCATCCCCACTGTTTTAAGAACAGTCAGAAATGTATTAAGGTGTCAAATGAAGATTGT
TACTAACGAACAAAAAAGGAGAGTCATTTGAACTGTCAGTAAGATGGCAACGTGGGGAAG
GGTGTCCTAAGTGCCTGGAAACACGCACAGCAATGCTAAGGACATGGGAGGGTAGAGTGA
TGCTGAAACACAGTCAACACCAACCAGAAAGGACGGGAATGTCTGTGAGGCTGCTGAAAA
GAGACCCCCAACGCTAATGTGCCTTATGGGTCCATGGGCAGACGCTGAACGCTGGCAAAA
CCGGGTGGCCGTTCTTGCACTACTCGGGTTCTGACTGCAGGAGGGGCAGAGGGTGGTCGA
TGTCCTAGTCCCTGTGTCTAAAAAGTCATGAGGGGACAGAGTACAACAGGCAGCACGTTA
AGTCCCTGGGCTCTTTTCCAAAGCCAAGAGGAGGCTCCCTGGAGAAGCGAGCTTTGCGAA
TGGCTCAGCCCGGAGCCGCACACTAACGCGGCGTGCANCAAGTGTGGCTGGGTGTGCACA
GCTGGCGTCCAGCGACAGCCT
Restriction Sites NotI-NotI |
ACCN NM_005116
Insert Size 6880 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005116.5, NP_005107.4
RefSeq Size 6953 bp
RefSeq ORF 1953 bp
Locus ID 9962
UniProt ID Q9UGH3
Cytogenetics 20p13
Domains xan_ur_permease
Protein Families Transmembrane
Summary The absorption of vitamin C into the body and its distribution to organs requires two sodium-dependent vitamin C transporters. This gene encodes one of the two required transporters and the encoded protein accounts for tissue-specific uptake of vitamin C. Previously, this gene had an official symbol of SLC23A1. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) occurs more frequently. Variants 1 and 2 encode the same isoform.
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Documents
Resources
"NM_005116" in other vectors (6)
SKU Description Size Price
RC216776 SLC23A2 (Myc-DDK-tagged)-Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1 10 ug
$907.00
RC216776L1 Lenti ORF clone of Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1, Myc-DDK-tagged 10 ug
$1,207.00
RC216776L2 Lenti ORF clone of Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1, mGFP tagged 10 ug
$1,207.00
RC216776L3 Lenti ORF clone of Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1, Myc-DDK-tagged 10 ug
$1,207.00
RC216776L4 Lenti ORF clone of Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1, mGFP tagged 10 ug
$1,207.00
RG216776 SLC23A2 (tGFP-tagged) - Human solute carrier family 23 (nucleobase transporters), member 2 (SLC23A2), transcript variant 1 10 ug
$1,107.00

file-alt Citations

L-Ascorbic acid can abrogate SVCT2-dependent cetuximab resistance mediated by mutant KRAS in human colon cancer cells
Jung, Lee, Moon et al
Free Radic. Biol. Med. (2016) 95, 200–208 DOI: 10.1016/j.freeradbiomed.2016.03.009
Cited by 47
AI Summary: In this study, OriGene products—specifically, human cDNA encoding SVCT-2 and myc-tagged catalase—were used to manipulate SVCT-2 and catalase expression in colon cancer cells, enabling the investigation of their roles in L-ascorbic acid and cetuximab treatment responses.
Snippet: 2.3. Plasmids, siRNA and transfection. Human cDNA encoding SVCT-2 or myc-tagged catalase was acquired from Origene (Rockville, MD, USA). We additionally constructed myc-tagged SVCT-2 from a SVCT-2-expressing plasmid using a myc-tagged pcDNA ... Cite SaveSaving
Showing 1-1 of 1 papers.
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