MAP2 (NM_002374) Human Tagged ORF Clone

SKU RC216775

MAP2 (Myc-DDK-tagged)-Human microtubule-associated protein 2 (MAP2), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MAP2
• Synonyms: MAP-2; MAP2A; MAP2B; MAP2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216775 representing NM_002374.; Blue=ORF Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCAGATGAACGGAAAGATGAAGCAAAGGCACCTCACTGGACCTCAGCACCGCTAACAGAGGCATCT; GCACACTCACATCCACCTGAGATTAAGGATCAAGGCGGAGCAGGGGAAGGACTTGTCCGAAGCGCTAAT; GGATTCCCATACAGGGAGGATGAAGAGGGTGCCTTTGGAGAGCATGGGTCACAGGGCACCTATTCAAAT; ACCAAAGAGAATGGGATCAACGGAGAGCTGACCTCAGCTGACAGAGAAACAGCAGAGGAGGTGTCTGCA; AGGATAGTTCAAGTAGTCACTGCTGAGGCTGTAGCAGTCCTGAAAGGTGAACAAGAGAAAGAAGCTCAA; CATAAAGACCAGACTGCAGCTCTGCCTTTAGCAGCTGAAGAAACAGCTAATCTGCCTCCTTCTCCACCC; CCATCACCTGCCTCAGAACAGACTGTCACAGTGGAGGAAGATTTACTTACAGCCTCGAAGATGGAGTTC; CACGATCAACAGGAATTGACTCCCTCTACAGCTGAGCCTTCAGACCAGAAGGAAAAGGAGTCAGAGAAG; CAAAGTAAGCCTGGTGAAGACCTTAAACATGCTGCCTTAGTTTCTCAGCCAGAGACAACTAAAACTTAC; CCTGATAAAAAGGACATGCAAGGCACGGAAGAAGAAAAAGCACCCCTAGCTTTGTTTGGGCACACTCTT; GTTGCCAGCCTGGAAGACATGAAACAGAAGACAGAACCAAGCCTTGTAGTACCTGGCATTGACCTCCCT; AAAGAGCCTCCAACTCCAAAAGAACAAAAGGACTGGTTCATCGAAATGCCAACGGAAGCAAAAAAGGAT; GAGTGGGGTTTAGTTGCCCCCATATCTCCTGGCCCTCTGACTCCCATGAGGGAAAAAGATGTATTTGAT; GATATCCCAAAATGGGAAGGGAAACAGTTTGATTCTCCCATGCCAAGTCCCTTTCAAGGGGGAAGCTTC; ACTCTTCCTTTAGATGTCATGAAGAATGAAATAGTTACAGAAACATCGCCCTTTGCCCCTGCCTTTTTA; CAGCCAGATGACAAAAAATCTCTGCAACAAACCAGTGGCCCAGCTACTGCCAAAGATAGTTTTAAAATT; GAAGAGCCCCATGAGGCTAAACCTGACAAAATGGCAGAAGCACCACCCTCAGAGGCAATGACCTTACCC; AAAGATGCTCACATTCCAGTTGTAGAAGAACATGTTATGGGGAAAGTTTTAGAGGAAGAAAAGGAGGCC; ATAAATCAAGAGACTGTGCAGCAAAGGGATACTTTCACCCCCAGTGGACAGGAACCTATACTTACTGAA; AAGGAAACTGAGCTGAAGCTTGAAGAAAAAACCACCATTTCTGACAAAGAAGCTGTGCCAAAAGAGAGT; AAACCCCCAAAACCTGCAGATGAAGAAATAGGCATAATTCAGACCTCCACAGAGCACACTTTCTCAGAA; CAGAAAGACCAAGAGCCTACCACAGATATGTTGAAACAGGACTCGTTCCCTGTAAGTTTGGAGCAAGCA; GTTACAGATTCAGCCATGACCTCTAAAACACTGGAGAAAGCCATGACCGAACCATCTGCATTAATTGAA; AAGAGCTCAATTCAGGAACTTTTTGAAATGAGAGTTGATGACAAAGATAAGATTGAAGGAGTTGGAGCT; GCAACATCAGCTGAGCTTGATATGCCATTTTATGAAGATAAATCAGGAATGTCCAAGTACTTTGAAACA; TCTGCCTTGAAAGAAGAAGCAACAAAAAGCATTGAGCCAGGCAGTGATTACTATGAACTGAGTGACACT; AGAGAAAGTGTCCATGAGTCTATTGATACCATGTCTCCCATGCATAAAAATGGTGACAAGGAGTTTCAA; ACAGGAAAAGAATCCCAGCCCAGTCCTCCAGCACAAGAAGCAGGGTACAGCACTCTCGCACAGAGTTAT; CCATCAGATTTACCTGAAGAACCCAGTTCTCCTCAAGAAAGAATGTTCACTATTGATCCAAAAGTGTAT; GGAGAGAAAAGGGACCTCCACAGTAAGAATAAGGATGATTTGACCCTTAGCAGGAGTTTAGGACTTGGT; GGTAGGTCTGCAATAGAACAAAGAAGCATGTCAATCAATTTGCCGATGTCTTGCCTAGATTCCATAGCC; CTTGGATTTAACTTTGGTCGGGGACATGATCTTTCTCCTCTGGCTTCCGATATTCTAACCAACACTAGT; GGAAGTATGGATGAAGGGGATGATTACCTTCCAGCCACCACACCTGCACTGGAGAAAGCCCCTTGCTTC; CCTGTAGAAAGCAAAGAGGAAGAACAGATAGAGAAAGTAAAAGCTACTGGAGAAGAAAGTACTCAAGCG; GAGATATCATGTGAGTCTCCTTTCCTAGCCAAAGATTTTTACAAAAATGGTACTGTCATGGCACCTGAC; CTTCCTGAAATGCTAGATCTGGCAGGCACAAGGTCAAGATTGGCTTCTGTGAGTGCAGATGCTGAGGTT; GCCAGGAGGAAATCAGTCCCATCAGAGACTGTGGTTGAGGATAGTCGTACTGGCTTGCCCCCGGTAACT; GATGAAAACCATGTCATTGTAAAAACGGACAGTCAGCTCGAAGACCTGGGCTACTGTGTGTTCAATAAG; TACACAGTCCCATTGCCATCACCTGTTCAAGACAGTGAGAATTTATCAGGGGAGAGTGGTACCTTTTAC; GAAGGCACTGATGATAAAGTTCGAAGAGATTTGGCCACAGACCTTTCACTGATTGAAGTGAAACTGGCA; GCAGCCGGAAGAGTCAAAGATGAGTTCAGTGTTGACAAAGAAGCATCCGCGCATATCTCTGGTGACAAA; TCAGGACTGAGTAAGGAGTTTGACCAAGAGAAGAAAGCTAATGATAGGTTGGATACTGTACTAGAAAAG; AGTGAAGAACATGCTGATTCAAAAGAACATGCCAAGAAAACTGAAGAGGCTGGTGATGAAATAGAAACA; TTCGGATTAGGAGTAACCTATGAGCAAGCTTTGGCCAAAGATTTGTCAATACCAACAGATGCATCCTCT; GAGAAAGCAGAGAAGGGTCTTAGTTCAGTGCCAGAGATAGCTGAGGTAGAACCATCCAAAAAGGTGGAA; CAAGGTCTGGATTTTGCTGTCCAGGGTCAACTAGATGTTAAAATTAGTGACTTTGGACAGATGGCTTCA; GGGCTAAACATAGATGATAGAAGGGCAACAGAGCTAAAACTTGAGGCTACACAGGACATGACCCCCTCA; TCCAAAGCACCGCAGGAGGCAGATGCATTTATGGGTGTTGAGTCTGGCCACATGAAAGAAGGCACTAAA; GTTAGTGAGACAGAAGTCAAAGAGAAGGTGGCCAAGCCTGACTTGGTGCACCAGGAGGCTGTAGACAAG; GAGGAGTCCTATGAATCTAGTGGTGAGCATGAAAGTCTCACCATGGAGTCCTTGAAAGCTGATGAGGGC; AAGAAGGAAACATCTCCAGAATCATCTCTAATTCAAGATGAGATTGCCGTCAAATTGTCAGTGGAAATA; CCTTGCCCACCTGCTGTTTCAGAGGCTGATTTAGCCACAGATGAGAGAGCTGATGTCCAGATGGAATTT; ATTCAGGGGCCAAAAGAAGAAAGCAAAGAGACCCCAGATATATCCATCACGCCTTCTGATGTTGCAGAG; CCATTGCATGAAACGATCGTATCTGAACCAGCAGAGATTCAGAGTGAGGAAGAAGAGATAGAAGCCCAG; GGAGAATATGATAAACTGCTCTTCCGCTCAGACACCCTTCAGATAACTGACCTGGGTGTCTCAGGTGCC; AGGGAGGAATTTGTGGAGACCTGCCCAAGTGAACACAAAGGAGTGATTGAGTCTGTTGTGACCATCGAG; GATGATTTCATCACTGTAGTGCAAACCACAACTGATGAAGGGGAGTCAGGGTCCCACAGCGTGCGTTTT; GCAGCCCTAGAGCAGCCTGAGGTGGAAAGGAGACCATCTCCTCATGATGAAGAAGAGTTTGAAGTAGAA; GAGGCAGCTGAAGCCCAGGCAGAACCCAAAGATGGTTCCCCAGAGGCTCCAGCTTCCCCTGAGAGAGAA; GAGGTTGCACTTTCTGAATATAAGACAGAAACCTATGACGATTACAAAGATGAGACCACCATTGACGAC; TCCATCATGGACGCTGACAGCCTCTGGGTGGACACTCAAGATGATGATAGGAGCATCATGACAGAACAG; TTAGAAACTATTCCTAAAGAGGAGAAAGCTGAAAAGGAAGCTCGGAGATCATCTCTTGAGAAACATAGA; AAAGAAAAGCCTTTTAAAACCGGGAGAGGCAGAATTTCCACTCCTGAAAGAAAAGTAGCTAAAAAGGAA; CCTAGCACAGTCTCCAGAGATGAAGTGAGAAGGAAAAAAGCAGTTTATAAGAAGGCTGAACTTGCTAAA; AAAACAGAAGTTCAGGCCCACTCTCCCTCCAGGAAATTCATTTTAAAACCTGCTATCAAATATACTAGA; CCAACTCATCTCTCCTGTGTTAAGCGGAAAACCACAGCAGCAGGTGGGGAATCAGCTCTGGCTCCCAGT; GTATTTAAACAGGCAAAGGACAAAGTCTCTGACGGAGTAACCAAGAGCCCAGAAAAGCGCTCTTCTCTC; CCAAGACCTTCCTCCATTCTCCCTCCTCGGCGAGGTGTGTCAGGAGACAGAGATGAGAATTCCTTCTCT; CTCAACAGTTCTATCTCTTCTTCAGCACGGCGGACCACCAGGTCAGAGCCAATTCGCAGAGCAGGGAAG; AGTGGTACCTCAACACCCACTACCCCTGGGTCTACTGCCATCACTCCTGGCACCCCACCAAGTTATTCT; TCACGCACACCAGGCACTCCTGGAACCCCTAGCTATCCCAGGACCCCTCACACACCAGGAACCCCCAAG; TCTGCCATCTTGGTGCCGAGTGAGAAGAAGGTCGCCATCATACGTACTCCTCCAAAATCTCCTGCGACT; CCCAAGCAGCTTCGGCTTATTAACCAACCACTGCCAGACCTGAAGAATGTCAAATCCAAAATCGGATCA; ACAGACAACATCAAATACCAGCCTAAAGGGGGGCAGGTACAAATTGTTACCAAGAAAATAGACCTAAGC; CATGTGACATCCAAATGTGGCTCTCTGAAGAACATCCGCCACAGGCCAGGTGGCGGACGTGTGAAAATT; GAGAGTGTAAAACTAGATTTCAAAGAAAAGGCCCAAGCTAAAGTTGGTTCTCTTGATAATGCTCATCAT; GTACCTGGAGGTGGTAATGTCAAGATTGACAGCCAAAAGTTGAACTTCAGAGAGCATGCTAAAGCCCGT; GTGGACCATGGGGCTGAGATCATTACACAGTCCCCAGGCAGATCCAGCGTGGCATCACCCCGACGACTC; AGCAATGTCTCCTCGTCTGGAAGCATCAACCTGCTCGAATCTCCTCAGCTTGCCACTTTGGCTGAGGAT; GTCACTGCTGCACTCGCTAAGCAGGGCTTG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
  Protein Sequence

  >Peptide sequence encoded by RC216775; Blue=ORF Red=Cloning site Green=Tag(s); ; MADERKDEAKAPHWTSAPLTEASAHSHPPEIKDQGGAGEGLVRSANGFPYREDEEGAFGEHGSQGTYSN; TKENGINGELTSADRETAEEVSARIVQVVTAEAVAVLKGEQEKEAQHKDQTAALPLAAEETANLPPSPP; PSPASEQTVTVEEDLLTASKMEFHDQQELTPSTAEPSDQKEKESEKQSKPGEDLKHAALVSQPETTKTY; PDKKDMQGTEEEKAPLALFGHTLVASLEDMKQKTEPSLVVPGIDLPKEPPTPKEQKDWFIEMPTEAKKD; EWGLVAPISPGPLTPMREKDVFDDIPKWEGKQFDSPMPSPFQGGSFTLPLDVMKNEIVTETSPFAPAFL; QPDDKKSLQQTSGPATAKDSFKIEEPHEAKPDKMAEAPPSEAMTLPKDAHIPVVEEHVMGKVLEEEKEA; INQETVQQRDTFTPSGQEPILTEKETELKLEEKTTISDKEAVPKESKPPKPADEEIGIIQTSTEHTFSE; QKDQEPTTDMLKQDSFPVSLEQAVTDSAMTSKTLEKAMTEPSALIEKSSIQELFEMRVDDKDKIEGVGA; ATSAELDMPFYEDKSGMSKYFETSALKEEATKSIEPGSDYYELSDTRESVHESIDTMSPMHKNGDKEFQ; TGKESQPSPPAQEAGYSTLAQSYPSDLPEEPSSPQERMFTIDPKVYGEKRDLHSKNKDDLTLSRSLGLG; GRSAIEQRSMSINLPMSCLDSIALGFNFGRGHDLSPLASDILTNTSGSMDEGDDYLPATTPALEKAPCF; PVESKEEEQIEKVKATGEESTQAEISCESPFLAKDFYKNGTVMAPDLPEMLDLAGTRSRLASVSADAEV; ARRKSVPSETVVEDSRTGLPPVTDENHVIVKTDSQLEDLGYCVFNKYTVPLPSPVQDSENLSGESGTFY; EGTDDKVRRDLATDLSLIEVKLAAAGRVKDEFSVDKEASAHISGDKSGLSKEFDQEKKANDRLDTVLEK; SEEHADSKEHAKKTEEAGDEIETFGLGVTYEQALAKDLSIPTDASSEKAEKGLSSVPEIAEVEPSKKVE; QGLDFAVQGQLDVKISDFGQMASGLNIDDRRATELKLEATQDMTPSSKAPQEADAFMGVESGHMKEGTK; VSETEVKEKVAKPDLVHQEAVDKEESYESSGEHESLTMESLKADEGKKETSPESSLIQDEIAVKLSVEI; PCPPAVSEADLATDERADVQMEFIQGPKEESKETPDISITPSDVAEPLHETIVSEPAEIQSEEEEIEAQ; GEYDKLLFRSDTLQITDLGVSGAREEFVETCPSEHKGVIESVVTIEDDFITVVQTTTDEGESGSHSVRF; AALEQPEVERRPSPHDEEEFEVEEAAEAQAEPKDGSPEAPASPEREEVALSEYKTETYDDYKDETTIDD; SIMDADSLWVDTQDDDRSIMTEQLETIPKEEKAEKEARRSSLEKHRKEKPFKTGRGRISTPERKVAKKE; PSTVSRDEVRRKKAVYKKAELAKKTEVQAHSPSRKFILKPAIKYTRPTHLSCVKRKTTAAGGESALAPS; VFKQAKDKVSDGVTKSPEKRSSLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGK; SGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPAT; PKQLRLINQPLPDLKNVKSKIGSTDNIKYQPKGGQVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKI; ESVKLDFKEKAQAKVGSLDNAHHVPGGGNVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRL; SNVSSSGSINLLESPQLATLAEDVTAALAKQGL; TRTRPLEQKLISEEDLAANDILDYKDDDDKV; ; Recombinant protein using RC216775 also available, TP316775
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002374
• ORF Size: 5481 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq Size: 6534 bp
• RefSeq ORF: 5484 bp
• Locus ID: 4133
• UniProt ID: P11137
• Cytogenetics: 2q34
• Domains: tubulin-binding
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS
• MW: 199.5 kDa
• Summary: This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]