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IKK alpha (CHUK) (NM_001278) Human Tagged ORF Clone

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SKU
RC216718
CHUK (Myc-DDK-tagged)-Human conserved helix-loop-helix ubiquitous kinase (CHUK)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $1,023.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IKK alpha
Synonyms BPS2; IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216718 representing NM_001278
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGGCCCTGGGAGATGCGGGAGCGGCTGGGCACCG
GCGGCTTCGGGAACGTCTGTCTGTACCAGCATCGGGAACTTGATCTCAAAATAGCAATTAAGTCTTGTCG
CCTAGAGCTAAGTACCAAAAACAGAGAACGATGGTGCCATGAAATCCAGATTATGAAGAAGTTGAACCAT
GCCAATGTTGTAAAGGCCTGTGATGTTCCTGAAGAATTGAATATTTTGATTCATGATGTGCCTCTTCTAG
CAATGGAATACTGTTCTGGAGGAGATCTCCGAAAGCTGCTCAACAAACCAGAAAATTGTTGTGGACTTAA
AGAAAGCCAGATACTTTCTTTACTAAGTGATATAGGGTCTGGGATTCGATATTTGCATGAAAACAAAATT
ATACATCGAGATCTAAAACCTGAAAACATAGTTCTTCAGGATGTTGGTGGAAAGATAATACATAAAATAA
TTGATCTGGGATATGCCAAAGATGTTGATCAAGGAAGTCTGTGTACATCTTTTGTGGGAACACTGCAGTA
TCTGGCCCCAGAGCTCTTTGAGAATAAGCCTTACACAGCCACTGTTGATTATTGGAGCTTTGGGACCATG
GTATTTGAATGTATTGCTGGATATAGGCCTTTTTTGCATCATCTGCAGCCATTTACCTGGCATGAGAAGA
TTAAGAAGAAGGATCCAAAGTGTATATTTGCATGTGAAGAGATGTCAGGAGAAGTTCGGTTTAGTAGCCA
TTTACCTCAACCAAATAGCCTTTGTAGTTTAGTAGTAGAACCCATGGAAAACTGGCTACAGTTGATGTTG
AATTGGGACCCTCAGCAGAGAGGAGGACCTGTTGACCTTACTTTGAAGCAGCCAAGATGTTTTGTATTAA
TGGATCACATTTTGAATTTGAAGATAGTACACATCCTAAATATGACTTCTGCAAAGATAATTTCTTTTCT
GTTACCACCTGATGAAAGTCTTCATTCACTACAGTCTCGTATTGAGCGTGAAACTGGAATAAATACTGGT
TCTCAAGAACTTCTTTCAGAGACAGGAATTTCTCTGGATCCTCGGAAACCAGCCTCTCAATGTGTTCTAG
ATGGAGTTAGAGGCTGTGATAGCTATATGGTTTATTTGTTTGATAAAAGTAAAACTGTATATGAAGGGCC
ATTTGCTTCCAGAAGTTTATCTGATTGTGTAAATTATATTGTACAGGACAGCAAAATACAGCTTCCAATT
ATACAGCTGCGTAAAGTGTGGGCTGAAGCAGTGCACTATGTGTCTGGACTAAAAGAAGACTATAGCAGGC
TCTTTCAGGGACAAAGGGCAGCAATGTTAAGTCTTCTTAGATATAATGCTAACTTAACAAAAATGAAGAA
CACTTTGATCTCAGCATCACAACAACTGAAAGCTAAATTGGAGTTTTTTCACAAAAGCATTCAGCTTGAC
TTGGAGAGATACAGCGAGCAGATGACGTATGGGATATCTTCAGAAAAAATGCTAAAAGCATGGAAAGAAA
TGGAAGAAAAGGCCATCCACTATGCTGAGGTTGGTGTCATTGGATACCTGGAGGATCAGATTATGTCTTT
GCATGCTGAAATCATGGAGCTACAGAAGAGCCCCTATGGAAGACGTCAGGGAGACTTGATGGAATCTCTG
GAACAGCGTGCCATTGATCTATATAAGCAGTTAAAACACAGACCTTCAGATCACTCCTACAGTGACAGCA
CAGAGATGGTGAAAATCATTGTGCACACTGTGCAGAGTCAGGACCGTGTGCTCAAGGAGCTGTTTGGTCA
TTTGAGCAAGTTGTTGGGCTGTAAGCAGAAGATTATTGATCTACTCCCTAAGGTGGAAGTGGCCCTCAGT
AATATCAAAGAAGCTGACAATACTGTCATGTTCATGCAGGGAAAAAGGCAGAAAGAAATATGGCATCTCC
TTAAAATTGCCTGTACACAGAGTTCTGCCCGGTCCCTTGTAGGATCCAGTCTAGAAGGTGCAGTAACCCC
TCAGACATCAGCATGGCTGCCCCCGACTTCAGCAGAACATGATCATTCTCTGTCATGTGTGGTAACTCCT
CAAGATGGGGAGACTTCAGCACAAATGATAGAAGAAAATTTGAACTGCCTTGGCCATTTAAGCACTATTA
TTCATGAGGCAAATGAGGAACAGGGCAATAGTATGATGAATCTTGATTGGAGTTGGTTAACAGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216718 protein sequence
Red=Cloning site Green=Tags(s)

MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNH
ANVVKACDVPEELNILIHDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKI
IHRDLKPENIVLQDVGGKIIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTM
VFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLVVEPMENWLQLML
NWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTG
SQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPI
IQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLD
LERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESL
EQRAIDLYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALS
NIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTP
QDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_001278
ORF Size 2235 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001278.5
RefSeq Size 3539 bp
RefSeq ORF 2238 bp
Locus ID 1147
UniProt ID O15111
Cytogenetics 10q24.31
Domains pkinase, S_TKc, TyrKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway
MW 84.5 kDa
Summary This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. provided by RefSeq, Jul 2008
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"NM_001278" in other vectors (7)
SKU Description Size Price
RC216718L1 Lenti ORF clone of Human conserved helix-loop-helix ubiquitous kinase (CHUK), Myc-DDK-tagged 10 ug
$1,323.00
RC216718L2 Lenti ORF clone of Human conserved helix-loop-helix ubiquitous kinase (CHUK), mGFP tagged 10 ug
$1,323.00
RC216718L3 Lenti ORF clone of Human conserved helix-loop-helix ubiquitous kinase (CHUK), Myc-DDK-tagged 10 ug
$1,323.00
RC216718L4 Lenti ORF clone of Human conserved helix-loop-helix ubiquitous kinase (CHUK), mGFP tagged 10 ug
$1,323.00
RG216718 CHUK (tGFP-tagged) - Human conserved helix-loop-helix ubiquitous kinase (CHUK) 10 ug
$789.00 $1,223.00
SC119358 CHUK (untagged)-Human conserved helix-loop-helix ubiquitous kinase (CHUK) 10 ug
$1,024.00
SC323326 CHUK (untagged)-Kinase deficient mutant (K44M) of Human conserved helix-loop-helix ubiquitous kinase (CHUK) 10 ug
$1,024.00

file-alt Citations

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