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IKK alpha (CHUK) Mouse Monoclonal Antibody [Clone ID: 14A231]

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SKU
TA336452
Mouse Monoclonal IKK alpha Antibody (14A231)
Size:
100 ug
Conjugation:
Unconjugated
  $680.00
5 Days*
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View other "IKK alpha or NM_001278" antibodies

Specifications
Specifications
Product Data
Clone Name 14A231
Application ChIP, FC, ICC/IF, IHC, IP, WB
Recommended Dilution Flow Cytometry: (Intracellular): 0.25-0.5 ug/10^6 cells, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Western Blot: 1 ug/ml, Simple Western: 10 ug/ml, Immunoprecipitation: 1-2 ug/ml, Flow (Intracellular): Flow Cytometry: (Intracellular): 0.25-0.5 ug/10^6 cells, Immunohistochemistry-Frozen, Chromatin Immunoprecipitation: 1:10 - 1:500, Immunohistochemistry: 1:200, Immunohistochemistry-Paraffin: 5ug/ml, Immunocytochemistry/ Immunofluorescence: 1:10
Reactivity Human, Mouse, Primate
Antibody Host Mouse
Isotype IgG1, kappa
Clonality Monoclonal
Immunogen This antibody was raised against a His-tagged full-length human IKK alpha protein.
Buffer PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Protein G purified
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Blue Ice
Gene Name conserved helix-loop-helix ubiquitous kinase
Database Link
Background NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. In vitro, IKK1/a and IKK2/b can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Targeted disruption of IKK1/a gene in mice results in skin and limb abnormalities and death of newborns.
Synonyms IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16
Note Use in Flow Intracellular reported in scientific literature (PMID 24804954)
Reference Data
Protein Categories Cytokines, Enzyme: Kinases, Intracellular Proteins
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway
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