PSRC1 (NM_001005290) Human Tagged ORF Clone

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SKU
RC216691
PSRC1 (Myc-DDK-tagged)-Human proline/serine-rich coiled-coil 1 (PSRC1), transcript variant 2
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PSRC1
Synonyms DDA3; FP3214
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216691 representing NM_001005290
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGATTTGGAGGAAGATGTAAGGTTTATTGTGGATGAGACCTTGGACTTTGGGGGGCTGTCACCAT
CTGACAGCCGTGAGGAGGAAGACATAACAGTGTTGGTGACTCCAGAGAAACCACTTCGACGGGGCCTCTC
CCACCGAAGTGACCCAAATGCAGTGGCACCTGCCCCCCAGGGTGTGAGGCTCAGCCTAGGCCCCCTCAGT
CCAGAGAAGCTGGAGGAGATCCTCGATGAGGCCAACCGGCTGGCCGCTCAGCTGGAGCAGTGTGCCCTGC
AGGATCGGGAGAGCGCAGGCGAGGGCCTGGGGCCTCGCCGAGTGAAGCCCAGTCCTCGGCGGGAGACCTT
TGTGCTGAAGGATAGTCCTGTCCGAGACCTGCTGCCCACTGTGAACTCTTTGACGCGGAGCACCCCCTCC
CCAAGCAGCCTGACGCCTCGACTCCGGAGTAATGATAGGAAGGGGTCAGTCAGGGCTCTCCGGGCTACAT
CTGGAAAGAGGCCCTCCAACATGAAGAGGGAGTCACCCACTTGCAATCTGTTCCCTGCATCCAAAAGCCC
AGCATCTTCTCCTCTTACCCGATCGACTCCCCCAGTCCGGGGGAGAGCCGGGCCCAGTGGGAGAGCAGCA
GCCAGTGAGGAGACCAGAGCAGCCAAGTTGCGGGCCTGCCAGCCGAATGCCACTCACCAGCCGGAGTGTG
CCACCTGGCAGAGGTGCCCTACCTCCGGATTCTCTGTCAACTCGAAAAGGGCTTCCAAGACCAAGCACTG
CAGGACACAGAGTGCGGGAAAGTGGACACAAGGTTCCTGTTTCCCAGCGACTAAATCTTCCTGTCATGGG
TGCCACTCGCAGCAATCTGCAGCCCCCCAGGAAAGTGGCAGTCCCAGGACCTACCAGGTAAAGAGATCAG
GACAGCAAGCAAGACTTCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC216691 representing NM_001005290
Red=Cloning site Green=Tags(s)

MEDLEEDVRFIVDETLDFGGLSPSDSREEEDITVLVTPEKPLRRGLSHRSDPNAVAPAPQGVRLSLGPLS
PEKLEEILDEANRLAAQLEQCALQDRESAGEGLGPRRVKPSPRRETFVLKDSPVRDLLPTVNSLTRSTPS
PSSLTPRLRSNDRKGSVRALRATSGKRPSNMKRESPTCNLFPASKSPASSPLTRSTPPVRGRAGPSGRAA
ASEETRAAKLRACQPNATHQPECATWQRCPTSGFSVNSKRASKTKHCRTQSAGKWTQGSCFPATKSSCHG
CHSQQSAAPQESGSPRTYQVKRSGQQARLQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001005290
ORF Size 930 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001005290.3, NP_001005290.1
RefSeq Size 1621 bp
RefSeq ORF 933 bp
Locus ID 84722
UniProt ID Q6PGN9
Cytogenetics 1p13.3
MW 33.5 kDa
Summary This gene encodes a proline-rich protein that is a target for regulation by the tumor suppressor protein p53. The encoded protein plays an important role in mitosis by recruiting and regulating microtubule depolymerases that destabalize microtubules. Alternatively spliced transcript variants encoding different isoforms have been described. provided by RefSeq, Apr 2014
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Documents
Resources
"NM_001005290" in other vectors (4)
SKU Description Size Price
RC216691L3 Lenti ORF clone of Human proline/serine-rich coiled-coil 1 (PSRC1), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC216691L4 Lenti ORF clone of Human proline/serine-rich coiled-coil 1 (PSRC1), transcript variant 2, mGFP tagged 10 ug
$600.00
RG216691 PSRC1 (tGFP-tagged) - Human proline/serine-rich coiled-coil 1 (PSRC1), transcript variant 2 10 ug
$500.00
SC300895 PSRC1 (untagged)-Human proline/serine-rich coiled-coil 1 (PSRC1), transcript variant 2 10 ug
$330.00

Plasmid Map for RC216691

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Cloning scheme for RC216691

Circular map for RC216691

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