PSRC1 (NM_001005290) Human Untagged Clone

SKU
SC300895
PSRC1 (untagged)-Human proline/serine-rich coiled-coil 1 (PSRC1), transcript variant 2
  $330.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PSRC1
Synonyms DDA3; FP3214
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC300895 representing NM_001005290.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAGGATTTGGAGGAAGATGTAAGGTTTATTGTGGATGAGACCTTGGACTTTGGGGGGCTGTCACCA
TCTGACAGCCGTGAGGAGGAAGACATAACAGTGTTGGTGACTCCAGAGAAACCACTTCGACGGGGCCTC
TCCCACCGAAGTGACCCAAATGCAGTGGCACCTGCCCCCCAGGGTGTGAGGCTCAGCCTAGGCCCCCTC
AGTCCAGAGAAGCTGGAGGAGATCCTCGATGAGGCCAACCGGCTGGCCGCTCAGCTGGAGCAGTGTGCC
CTGCAGGATCGGGAGAGCGCAGGCGAGGGCCTGGGGCCTCGCCGAGTGAAGCCCAGTCCTCGGCGGGAG
ACCTTTGTGCTGAAGGATAGTCCTGTCCGAGACCTGCTGCCCACTGTGAACTCTTTGACGCGGAGCACC
CCCTCCCCAAGCAGCCTGACGCCTCGACTCCGGAGTAATGATAGGAAGGGGTCAGTCAGGGCTCTCCGG
GCTACATCTGGAAAGAGGCCCTCCAACATGAAGAGGGAGTCACCCACTTGCAATCTGTTCCCTGCATCC
AAAAGCCCAGCATCTTCTCCTCTTACCCGATCGACTCCCCCAGTCCGGGGGAGAGCCGGGCCCAGTGGG
AGAGCAGCAGCCAGTGAGGAGACCAGAGCAGCCAAGTTGCGGGCCTGCCAGCCGAATGCCACTCACCAG
CCGGAGTGTGCCACCTGGCAGAGGTGCCCTACCTCCGGATTCTCTGTCAACTCGAAAAGGGCTTCCAAG
ACCAAGCACTGCAGGACACAGAGTGCGGGAAAGTGGACACAAGGTTCCTGTTTCCCAGCGACTAAATCT
TCCTGTCATGGGTGCCACTCGCAGCAATCTGCAGCCCCCCAGGAAAGTGGCAGTCCCAGGACCTACCAG
GTAAAGAGATCAGGACAGCAAGCAAGACTTCAGTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001005290
Insert Size 933 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001005290.3
RefSeq Size 1653 bp
RefSeq ORF 933 bp
Locus ID 84722
UniProt ID Q6PGN9
Cytogenetics 1p13.3
MW 33.6 kDa
Summary This gene encodes a proline-rich protein that is a target for regulation by the tumor suppressor protein p53. The encoded protein plays an important role in mitosis by recruiting and regulating microtubule depolymerases that destabalize microtubules. Alternatively spliced transcript variants encoding different isoforms have been described. provided by RefSeq, Apr 2014
Transcript Variant: This variant (2) uses two different alternate splice sites, which results in a frameshift, compared to variant 1. It encodes isoform b which has a shorter and distinct C-terminus, compared to isoform a.
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Documents
Resources
"NM_001005290" in other vectors (4)
SKU Description Size Price
RC216691 PSRC1 (Myc-DDK-tagged)-Human proline/serine-rich coiled-coil 1 (PSRC1), transcript variant 2 10 ug
$300.00
RC216691L3 Lenti ORF clone of Human proline/serine-rich coiled-coil 1 (PSRC1), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC216691L4 Lenti ORF clone of Human proline/serine-rich coiled-coil 1 (PSRC1), transcript variant 2, mGFP tagged 10 ug
$600.00
RG216691 PSRC1 (tGFP-tagged) - Human proline/serine-rich coiled-coil 1 (PSRC1), transcript variant 2 10 ug
$500.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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