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PLAG1 (NM_002655) Human Tagged ORF Clone

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SKU
RC216628
PLAG1 (Myc-DDK-tagged)-Human pleiomorphic adenoma gene 1 (PLAG1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PLAG1
Synonyms PSA; SGPA; SRS4; ZNF912
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216628 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACTGTCATTCCTGGTGATTTGTCAGAAGTAAGAGATACCCAGAAAGTCCCTTCAGGGAAACGTA
AGCGTGGTGAAACCAAACCAAGAAAAAACTTTCCTTGCCAACTGTGTGACAAGGCCTTTAACAGTGTTGA
GAAATTAAAGGTTCACTCCTACTCTCACACAGGAGAGAGGCCCTACAAGTGCATACAACAAGACTGCACC
AAGGCCTTTGTTTCTAAGTACAAATTACAAAGGCACATGGCTACTCATTCTCCTGAGAAAACCCACAAGT
GTAATTATTGTGAGAAAATGTTTCACCGGAAAGATCATCTGAAGAATCACCTCCATACACACGACCCTAA
CAAAGAGACGTTTAAGTGCGAAGAATGTGGCAAGAACTACAATACCAAGCTTGGATTTAAACGTCACTTG
GCCTTGCATGCCGCAACAAGTGGTGACCTCACCTGTAAGGTATGTTTGCAAACTTTTGAAAGCACGGGAG
TGCTTCTGGAGCACCTTAAATCTCATGCAGGCAAGTCGTCTGGTGGGGTTAAAGAAAAAAAGCACCAGTG
CGAACATTGTGATCGCCGGTTCTACACCCGAAAGGATGTCCGGAGACACATGGTGGTGCACACTGGAAGA
AAGGACTTCCTCTGTCAGTATTGTGCACAGAGATTTGGGCGAAAGGATCACCTGACTCGACATATGAAGA
AGAGTCACAATCAAGAGCTTCTGAAGGTCAAAACAGAACCAGTGGATTTCCTTGACCCATTTACCTGCAA
TGTGTCTGTGCCTATAAAAGACGAGCTCCTTCCGGTGATGTCCTTACCTTCCAGTGAACTGTTATCAAAG
CCATTCACAAACACTTTGCAGTTAAACCTCTACAACACTCCATTTCAGTCCATGCAGAGCTCGGGATCTG
CCCACCAAATGATCACAACTTTACCTTTGGGAATGACATGCCCAATAGATATGGACACTGTTCATCCCTC
TCACCACCTTTCTTTCAAATATCCGTTCAGTTCTACCTCATATGCAATTTCTATTCCTGAAAAAGAACAG
CCATTAAAGGGGGAAATTGAGAGTTACCTGATGGAGTTACAAGGTGGCGTGCCCTCTTCATCCCAAGATT
CTCAAGCATCGTCATCATCTAAGCTAGGGTTGGATCCTCAGATTGGGTCCCTAGATGATGGTGCAGGAGA
CCTCTCCCTATCCAAAAGCTCTATCTCCATCAGTGACCCCCTAAACACACCAGCATTGGATTTTTCTCAG
TTGTTTAATTTCATACCTTTAAATGGTCCTCCCTATAATCCTCTATCAGTGGGGAGCCTTGGAATGAGCT
ATTCCCAGGAAGAAGCACATTCTTCTGTTTCCCAGCTCCCCCCACAAACACAGGATCTTCAGGATCCTGC
AAACACTATAGGGCTTGGGTCTCTGCACTCACTGTCAGCAGCTTTCACCAGCAGTTTAAGCACAAGTACC
ACCCTCCCACGTTTCCATCAAGCTTTTCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216628 protein sequence
Red=Cloning site Green=Tags(s)

MATVIPGDLSEVRDTQKVPSGKRKRGETKPRKNFPCQLCDKAFNSVEKLKVHSYSHTGERPYKCIQQDCT
KAFVSKYKLQRHMATHSPEKTHKCNYCEKMFHRKDHLKNHLHTHDPNKETFKCEECGKNYNTKLGFKRHL
ALHAATSGDLTCKVCLQTFESTGVLLEHLKSHAGKSSGGVKEKKHQCEHCDRRFYTRKDVRRHMVVHTGR
KDFLCQYCAQRFGRKDHLTRHMKKSHNQELLKVKTEPVDFLDPFTCNVSVPIKDELLPVMSLPSSELLSK
PFTNTLQLNLYNTPFQSMQSSGSAHQMITTLPLGMTCPIDMDTVHPSHHLSFKYPFSSTSYAISIPEKEQ
PLKGEIESYLMELQGGVPSSSQDSQASSSSKLGLDPQIGSLDDGAGDLSLSKSSISISDPLNTPALDFSQ
LFNFIPLNGPPYNPLSVGSLGMSYSQEEAHSSVSQLPPQTQDLQDPANTIGLGSLHSLSAAFTSSLSTST
TLPRFHQAFQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002655
ORF Size 1500 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002655.3
RefSeq Size 7338 bp
RefSeq ORF 1503 bp
Locus ID 5324
UniProt ID Q6DJT9, A0A024R7Z0
Cytogenetics 8q12.1
Domains zf-C2H2
Protein Families Transcription Factors
MW 55.9 kDa
Summary Pleomorphic adenoma gene 1 encodes a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Three transcript variants encoding two different isoforms have been found for this gene. provided by RefSeq, Jul 2008
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Resources
"NM_002655" in other vectors (6)
SKU Description Size Price
RC216628L1 Lenti ORF clone of Human pleiomorphic adenoma gene 1 (PLAG1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC216628L2 Lenti ORF clone of Human pleiomorphic adenoma gene 1 (PLAG1), transcript variant 1, mGFP tagged 10 ug
$757.00
RC216628L3 Lenti ORF clone of Human pleiomorphic adenoma gene 1 (PLAG1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC216628L4 Lenti ORF clone of Human pleiomorphic adenoma gene 1 (PLAG1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG216628 PLAG1 (tGFP-tagged) - Human pleiomorphic adenoma gene 1 (PLAG1), transcript variant 1 10 ug
$489.00 $657.00
SC310316 PLAG1 (untagged)-Human pleiomorphic adenoma gene 1 (PLAG1), transcript variant 1 10 ug
$513.00

Plasmid Map for RC216628

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216628
Circular map for RC216628

file-alt Citations

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