SPATIAL (TBATA) (NM_152710) Human Tagged ORF Clone

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SKU
RC216613
TBATA (Myc-DDK-tagged)-Human chromosome 10 open reading frame 27 (C10orf27)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPATIAL
Synonyms C10orf27; SPATIAL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216613 representing NM_152710
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTACAGATGTTCAATTGGCTGGTTATCCACTGATGAGTCCAAAGGCTGAGCTGAAACTGGAGAAGA
AGTCAGGGCGCAAGCCAAGGAGCCCCAGGGACAGTGGGCCACAGAAAGAACTGGTGATCCCAGGGATTGT
GGATTTCGAGCGGATCCGCCGGGCATTGAGGACCCCAAAGCCCCAAACCCCTGGCACCTACTGCTTTGGA
CGCCTCAGTCACCACTCCTTCTTCTCCCGGCACCACCCACACCCCCAGCACGTGACCCACATCCAAGATC
TCACCGGGAAGCCTGTCTGTGTCGTCAGGGATTTTCCAGCCCCCTTGCCTGAGTCAACTGTCTTTTCCGG
CTGTCAAATGGGGATACCCACCATCTCTGTCCCCATTGGAGACCCACAGTCTAATCGGAACCCCCAGCTT
TCTTCTGAAGCCTGGAAGAAGGAGTTGAAGGAGCTAGCTTCCCGGGTGGCCTTCCTCACCAAGGAGGATG
AACTGAAGAAGAAAGAGAAGGAGCAGAAGGAGGAGCCTCTGCGGGAGCAGGGGGCAAAGTACTCAGCAGA
GACTGGGAGGCTCATCCCCGCTTCCACCCGGGCTGTCGGCCGCCGCAGATCTCACCAGGGCCAGCAGAGT
CAGTCTTCCAGCAGACATGAAGGAGTCCAGGCCTTCCTCCTTCAGGATCAGGAGCTGCTGGTCCTGGAGC
TCCTGTGTCAGATCCTGGAAACAGACTTGCTAAGCGCAATCCAGTTCTGGCTGCTCTACGCTCCGCCCAA
GGAAAAAGACCTCGCTCTGGGACTCCTGCAGACAGCAGTGGCTCAGCTCCTTCCCCAGCCCCTAGTCTCC
ATCCCTACAGAAAAGCTCCTAAGCCAGCTTCCAGAAGTGCATGAACCTCCTCAAGAGAAGCAAGAGCCAC
CCTGCAGTCAATCCCCGAAGAAAACGAAGATATCACCTTTTACAAAAAGCGAAAAACCAGAGTACATTGG
AGAAGCTCAAGTCCTCCAGATGCATTCAAGCCAGAACACAGAGAAGAAGACATCGAAGCCGAGGGCAGAG
AGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC216613 representing NM_152710
Red=Cloning site Green=Tags(s)

MATDVQLAGYPLMSPKAELKLEKKSGRKPRSPRDSGPQKELVIPGIVDFERIRRALRTPKPQTPGTYCFG
RLSHHSFFSRHHPHPQHVTHIQDLTGKPVCVVRDFPAPLPESTVFSGCQMGIPTISVPIGDPQSNRNPQL
SSEAWKKELKELASRVAFLTKEDELKKKEKEQKEEPLREQGAKYSAETGRLIPASTRAVGRRRSHQGQQS
QSSSRHEGVQAFLLQDQELLVLELLCQILETDLLSAIQFWLLYAPPKEKDLALGLLQTAVAQLLPQPLVS
IPTEKLLSQLPEVHEPPQEKQEPPCSQSPKKTKISPFTKSEKPEYIGEAQVLQMHSSQNTEKKTSKPRAE
S

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152710
ORF Size 1053 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152710.1, NP_689923.1
RefSeq Size 1583 bp
RefSeq ORF 1056 bp
Locus ID 219793
UniProt ID Q96M53
Cytogenetics 10q22.1
MW 39.3 kDa
Summary This gene encodes a protein that regulates thymic epithelial cell proliferation and thymus size. It has been identified as a ligand for the class I human leukocyte antigen (HLA-I) in thymus. Studies of the orthologous mouse protein suggest that it may also play a role in spermatid differentiation, as well as in neuronal morphogenesis and synaptic plasticity. Polymorphisms in this gene are associated with susceptibility for multiple sclerosis (MS). Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2015
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Documents
Resources
"NM_152710" in other vectors (4)
SKU Description Size Price
RC216613L3 Lenti ORF clone of Human chromosome 10 open reading frame 27 (C10orf27), Myc-DDK-tagged 10 ug
$757.00
RC216613L4 Lenti ORF clone of Human chromosome 10 open reading frame 27 (C10orf27), mGFP tagged 10 ug
$757.00
RG216613 TBATA (tGFP-tagged) - Human chromosome 10 open reading frame 27 (C10orf27) 10 ug
$489.00 $657.00
SC306488 TBATA (untagged)-Human chromosome 10 open reading frame 27 (C10orf27) 10 ug
$457.00

Plasmid Map for RC216613

Download sequence for

Cloning scheme for RC216613

Circular map for RC216613

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