OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

WIPI2 (NM_001033520) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC216506
WIPI2 (Myc-DDK-tagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 5
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA367805 WIPI2 rabbit polyclonal antibody
100 ul
$310.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "WIPI2 or NM_001033520"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol WIPI2
Synonyms ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216506 representing NM_001033520
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCACGCATGTACTTCCCTTTGTGATTTCAGCCGATACGGAAGATGTGTGCATTGTAGAGAGATTGT
TCTCCAGCAGCCTAGTGGCCATCGTCAGCCTTAAAGCACCAAGGAAGCTAAAGGTTTGCCACTTTAAGAA
GGGAACTGAGATCTGCAACTACAGCTACTCCAACACGATTCTGGCTGTGAAGCTCAACAGGCAGAGGCTG
ATAGTATGCCTGGAGGAGTCCCTGTACATCCACAACATTCGGGACATGAAGGTGCTGCATACGATCAGGG
AGACGCCTCCAAACCCTGCAGGCCTGTGTGCGCTGTCAATCAACAACGACAACTGCTACTTGGCGTACCC
AGGGAGCGCGACCATCGGAGAGGTGCAGGTCTTCGATACCATTAATTTGAGAGCTGCAAACATGATTCCG
GCTCACGACAGTCCTTTAGCGGCACTGGCCTTTGACGCAAGTGGAACTAAACTTGCCACGGCTTCGGAGA
AGGGGACCGTGATTAGGGTATTTTCCATTCCAGAAGGACAAAAACTCTTTGAGTTTCGGAGAGGAGTAAA
GAGGTGCGTGAGCATCTGCTCCCTGGCCTTCAGCATGGACGGCATGTTCCTCTCCGCCTCCAGCAACACT
GAGACCGTGCACATCTTCAAACTCGAGACTGTGAAAGAAAAACCCCCAGAGGAGCCCACCACCTGGACCG
GGTACTTCGGGAAAGTGCTCATGGCCTCCACCAGCTACCTGCCTTCCCAAGTGACAGAAATGTTCAACCA
GGGCAGAGCCTTCGCCACGGTCCGCCTGCCATTCTGCGGCCACAAAAACATCTGCTCGCTAGCCACAATT
CAGAAGATCCCGCGGTTGTTGGTGGGTGCCGCCGACGGGTACCTGTACATGTACAACCTGGACCCCCAGG
AGGGCGGCGAGTGTGCCCTGATGAAGCAGCACCGGCTGGACGGCAGTCTGGAAACGACCAATGAGATCTT
GGACTCTGCCTCTCACGACTGCCCCTTAGTCACTCAGACATACGGCGCAGCTGCAGGAAAAGCCTACACA
GACGACCTGGGTGCTGTGGGTGGCGCCTGCCTGGAGGACGAGGCCAGCGCCCTGCGCCTGGATGAGGACA
GCGAGCACCCGCCCATGATTCTTCGGACTGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216506 representing NM_001033520
Red=Cloning site Green=Tags(s)

MFTHVLPFVISADTEDVCIVERLFSSSLVAIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRL
IVCLEESLYIHNIRDMKVLHTIRETPPNPAGLCALSINNDNCYLAYPGSATIGEVQVFDTINLRAANMIP
AHDSPLAALAFDASGTKLATASEKGTVIRVFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNT
ETVHIFKLETVKEKPPEEPTTWTGYFGKVLMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLATI
QKIPRLLVGAADGYLYMYNLDPQEGGECALMKQHRLDGSLETTNEILDSASHDCPLVTQTYGAAAGKAYT
DDLGAVGGACLEDEASALRLDEDSEHPPMILRTD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033520
ORF Size 1152 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033520.1, NP_001028692.1
RefSeq Size 4295 bp
RefSeq ORF 1155 bp
Locus ID 26100
UniProt ID Q9Y4P8
Cytogenetics 7p22.1
MW 41.9 kDa
Summary WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 PubMed 15602573).supplied by OMIM, Mar 2008
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001033520" in other vectors (4)
SKU Description Size Price
RC216506L3 Lenti-ORF clone of WIPI2 (Myc-DDK-tagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 5 10 ug
$757.00
RC216506L4 Lenti-ORF clone of WIPI2 (mGFP-tagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 5 10 ug
$757.00
RG216506 WIPI2 (tGFP-tagged) - Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 5 10 ug
$657.00
SC302717 WIPI2 (untagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 5 10 ug
$503.00

Plasmid Map for RC216506

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216506
Circular map for RC216506

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products