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TBC1D3 (TBC1D3F) (NM_032258) Human Tagged ORF Clone

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SKU
RC216396
TBC1D3F (Myc-DDK-tagged)-Human TBC1 domain family, member 3F (TBC1D3F)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $511.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TBC1D3
Synonyms TBC1D3; TBC1D3G
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216396 representing NM_032258
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGTGGTAGAGGTCGCGGGCAGTTGGTGGGCACAAGAGCGAGAGGACATCATTATGAAATACGAAA
AGGGACACCGAGCTGGGCTGCCAGAGGACAAGGGGCCTAAGCCTTTTCGAAGCTACAACAACAACGTCGA
TCATTTGGGGATTGTACATGAGACGGAGCTGCCTCCTCTGACTGCGCGGGAGGCGAAGCAAATTCGGCGG
GAGATCAGCCGAAAGAGCAAGTGGGTGGATATGCTGGGAGACTGGGAGAAATACAAAAGCAGCAGAAAGC
TCATAGATCGAGCGTACAAGGGAATGCCCATGAACATCCGGGGCCCGATGTGGTCAGTCCTCCTGAACAC
TGAGGAAATGAAGTTGAAAAACCCCGGAAGATACCAGATCATGAAGGAGAAGGGCAAGAGGTCATCTGAG
CACATCCAGCGCATCGACCGGGACGTAAGCGGGACATTAAGGAAGCATATATTCTTCAGGGATCGATACG
GAACCAAGCAGCGGGAACTACTCCACATCCTCCTGGCATATGAGGAGTATAACCCGGAGGTGGGCTACTG
CAGGGACCTGAGCCACATCGCCGCCTTGTTCCTCCTCTATCTTCCTGAGGAGGATGCATTCTGGGCACTG
GTGCAGCTGCTGGCCAGTGAGAGGCACTCCCTGCAGGGATTTCACAGCCCAAATGGCGGGACCGTCCAGG
GGCTCCAAGACCAACAGGAGCATGTGGTAGCCACGTCACAACCCAAGACCATGGGGCATCAGGACAAGAA
AGATCTATGTGGGCAGTGTTCCCCGTTAGGCTGCCTCATCCGGATATTGATTGACGGGATCTCTCTCGGG
CTCACCCTGCGCCTGTGGGACGTGTATCTGGTAGAAGGCGAACAGGCGTTGATGCCGATAACAAGAATCG
CCTTTAAGGTTCAGCAGAAGCGCCTCACGAAGACGTCCAGGTGTGGCCCGTGGGCACGTTTTTGCAACCG
GTTCGTTGATACCTGGGCCAGGGATGAGGACACTGTGCTCAAGCATCTTAGGGCCTCTATGAAGAAACTA
ACAAGAAAGCAGGGGGACCTGCCACCCCCAGCCAAACCCGAGCAAGGGTCGTCGGCATCCAGGCCTGTGC
CGGCTTCACGTGGCGGGAAGACCCTCTGCAAGGGGGACAGGCAGGCCCCTCCAGGCCCACCAGCCCGGTT
CCCGCGGCCCATTTGGTCAGCTTCCCCGCCACGGGCACCTCGTTCTTCCACACCCTGTCCTGGTGGGGCT
GTCCGGGAAGACACCTACCCTGTGGGCACTCAGGGTGTGCCCAGCCCGGCCCTGGCTCAGGGAGGACCTC
AGGGTTCCTGGAGATTCCTGCAGTGGAACTCCATGCCCCGCCTCCCAACGGACCTGGACGTAGAGGGCCC
TTGGTTCCGCCATTATGATTTCAGACAGAGCTGCTGGGTCCGTGCCATATCCCAGGAGGACCAGCTGGCC
CCCTGCTGGCAGGCTGAACACCCTGCGGAGCGGGTGAGATCGGCTTTCGCTGCACCCAGCACTGATTCCG
ACCAGGGCACCCCCTTCAGAGCTAGGGACGAACAGCCGTGTGCTCCCACCTCAGGGCCTTGCCTCTGCGG
CCTCCACTTGGAAAGTTCTCAGTTCCCTCCAGGCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216396 representing NM_032258
Red=Cloning site Green=Tags(s)

MDVVEVAGSWWAQEREDIIMKYEKGHRAGLPEDKGPKPFRSYNNNVDHLGIVHETELPPLTAREAKQIRR
EISRKSKWVDMLGDWEKYKSSRKLIDRAYKGMPMNIRGPMWSVLLNTEEMKLKNPGRYQIMKEKGKRSSE
HIQRIDRDVSGTLRKHIFFRDRYGTKQRELLHILLAYEEYNPEVGYCRDLSHIAALFLLYLPEEDAFWAL
VQLLASERHSLQGFHSPNGGTVQGLQDQQEHVVATSQPKTMGHQDKKDLCGQCSPLGCLIRILIDGISLG
LTLRLWDVYLVEGEQALMPITRIAFKVQQKRLTKTSRCGPWARFCNRFVDTWARDEDTVLKHLRASMKKL
TRKQGDLPPPAKPEQGSSASRPVPASRGGKTLCKGDRQAPPGPPARFPRPIWSASPPRAPRSSTPCPGGA
VREDTYPVGTQGVPSPALAQGGPQGSWRFLQWNSMPRLPTDLDVEGPWFRHYDFRQSCWVRAISQEDQLA
PCWQAEHPAERVRSAFAAPSTDSDQGTPFRARDEQPCAPTSGPCLCGLHLESSQFPPGF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032258
ORF Size 1647 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032258.3
RefSeq Size 2072 bp
RefSeq ORF 1650 bp
Locus ID 84218
UniProt ID A6NER0
Cytogenetics 17q12
MW 62 kDa
Summary Acts as a GTPase activating protein for RAB5. Does not act on RAB4 or RAB11 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_032258" in other vectors (4)
SKU Description Size Price
RC216396L3 Lenti ORF clone of Human TBC1 domain family, member 3F (TBC1D3F), Myc-DDK-tagged 10 ug
$811.00
RC216396L4 Lenti ORF clone of Human TBC1 domain family, member 3F (TBC1D3F), mGFP tagged 10 ug
$811.00
RG216396 TBC1D3F (tGFP-tagged) - Human TBC1 domain family, member 3F (TBC1D3F) 10 ug
$711.00
SC305421 TBC1D3F (untagged)-Human TBC1 domain family, member 3F (TBC1D3F) 10 ug
$563.00

Plasmid Map for RC216396

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216396
Circular map for RC216396

file-alt Citations

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