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SLC6A11 (NM_014229) Human Tagged ORF Clone

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SKU
RC216392
SLC6A11 (Myc-DDK-tagged)-Human solute carrier family 6 (neurotransmitter transporter, GABA), member 11 (SLC6A11)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $588.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC6A11
Synonyms GAT-3; GAT3; GAT4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216392 representing NM_014229
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGCGGAGAAGGCGCTGCCCCTGGGCAATGGGAAGGCTGCTGAGGAGGCGCGGGAGTCCGAGGCGC
CGGGTGGCGGCTGCAGCAGCGGGGGCGCGGCGCCCGCGCGCCACCCGCGCGTCAAGCGCGACAAGGCGGT
CCACGAGCGCGGCCACTGGAACAACAAGGTGGAGTTCGTGCTGAGCGTGGCCGGGGAGATCATTGGGCTG
GGCAACGTGTGGCGCTTCCCCTACCTGTGCTACAAGAACGGAGGAGGGGCATTCCTGATTCCCTACGTGG
TGTTTTTTATTTGCTGTGGAATTCCTGTTTTTTTCCTGGAGACAGCTCTGGGGCAGTTCACAAGTGAAGG
TGGCATTACGTGTTGGAGGAAAGTTTGCCCTTTATTTGAAGGCATTGGCTATGCAACACAGGTGATTGAG
GCCCATCTGAATGTGTACTACATCATCATCCTGGCATGGGCCATTTTTTACCTGAGCAACTGCTTCACTA
CTGAGCTACCCTGGGCTACCTGTGGGCATGAGTGGAACACAGAGAATTGTGTGGAGTTCCAGAAACTGAA
TGTGAGCAACTACAGCCATGTGTCTCTGCAGAATGCCACCTCCCCTGTCATGGAGTTTTGGGAGCACCGG
GTCCTGGCCATCTCTGACGGGATCGAGCACATCGGGAACCTTCGCTGGGAGCTGGCCTTGTGTCTCTTGG
CAGCCTGGACCATCTGTTACTTCTGTATCTGGAAGGGGACCAAGTCTACAGGAAAGGTTGTATACGTGAC
TGCGACATTCCCCTACATCATGCTGCTGATCCTCCTGATACGAGGGGTCACGTTGCCCGGGGCCTCAGAG
GGCATCAAGTTCTACTTGTACCCTGACCTCTCCCGGCTCTCCGACCCCCAGGTCTGGGTAGATGCTGGAA
CGCAGATCTTTTTCTCCTATGCCATTTGCCTGGGCTGTCTGACCGCTCTGGGAAGTTATAACAATTATAA
CAACAACTGCTACAGGGACTGCATCATGCTCTGTTGCCTGAACAGCGGCACCAGCTTCGTGGCTGGGTTT
GCCATCTTCTCAGTCCTGGGTTTTATGGCGTACGAGCAGGGGGTACCCATTGCTGAGGTGGCAGAGTCAG
GCCCCGGCCTGGCCTTTATTGCGTACCCCAAGGCGGTCACCATGATGCCTCTCTCCCCGCTGTGGGCCAC
CTTGTTCTTCATGATGCTCATCTTCCTGGGCCTGGACAGCCAGTTTGTGTGTGTGGAAAGCCTGGTGACC
GCCGTGGTGGACATGTACCCCAAGGTTTTCCGGAGGGGTTACCGGCGGGAGCTGCTCATCCTAGCCTTGT
CTGTTATCTCCTATTTTCTGGGCCTCGTGATGTTAACAGAGGGTGGCATGTACATCTTCCAGCTCTTTGA
CTCCTATGCCGCCAGTGGGATGTGCCTTCTCTTCGTGGCCATCTTTGAGTGCATCTGCATCGGCTGGGTG
TATGGAAGCAACCGGTTCTATGATAACATTGAAGACATGATTGGCTACCGGCCACCGTCGCTCATTAAGT
GGTGCTGGATGATCATGACCCCTGGGATCTGCGCGGGGATCTTCATCTTCTTCTTGATCAAGTACAAGCC
ACTCAAGTACAACAACATCTACACCTACCCAGCCTGGGGCTATGGCATTGGCTGGCTCATGGCCCTGTCC
TCCATGCTCTGCATCCCGCTCTGGATCTGCATCACAGTGTGGAAGACGGAGGGGACACTGCCCGAGAAAC
TCCAGAAGTTGACGACCCCCAGCACAGATCTGAAAATGCGGGGCAAGCTTGGGGTGAGCCCACGGATGGT
GACAGTTAATGACTGTGATGCCAAACTCAAGAGTGACGGGACCATCGCAGCCATCACAGAGAAGGAGACG
CACTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216392 representing NM_014229
Red=Cloning site Green=Tags(s)

MTAEKALPLGNGKAAEEARESEAPGGGCSSGGAAPARHPRVKRDKAVHERGHWNNKVEFVLSVAGEIIGL
GNVWRFPYLCYKNGGGAFLIPYVVFFICCGIPVFFLETALGQFTSEGGITCWRKVCPLFEGIGYATQVIE
AHLNVYYIIILAWAIFYLSNCFTTELPWATCGHEWNTENCVEFQKLNVSNYSHVSLQNATSPVMEFWEHR
VLAISDGIEHIGNLRWELALCLLAAWTICYFCIWKGTKSTGKVVYVTATFPYIMLLILLIRGVTLPGASE
GIKFYLYPDLSRLSDPQVWVDAGTQIFFSYAICLGCLTALGSYNNYNNNCYRDCIMLCCLNSGTSFVAGF
AIFSVLGFMAYEQGVPIAEVAESGPGLAFIAYPKAVTMMPLSPLWATLFFMMLIFLGLDSQFVCVESLVT
AVVDMYPKVFRRGYRRELLILALSVISYFLGLVMLTEGGMYIFQLFDSYAASGMCLLFVAIFECICIGWV
YGSNRFYDNIEDMIGYRPPSLIKWCWMIMTPGICAGIFIFFLIKYKPLKYNNIYTYPAWGYGIGWLMALS
SMLCIPLWICITVWKTEGTLPEKLQKLTTPSTDLKMRGKLGVSPRMVTVNDCDAKLKSDGTIAAITEKET
HF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014229
ORF Size 1896 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014229.3
RefSeq Size 1991 bp
RefSeq ORF 1899 bp
Locus ID 6538
UniProt ID P48066
Cytogenetics 3p25.3
Domains SNF
Protein Families Druggable Genome, Transmembrane
MW 70.4 kDa
Summary The protein encoded by this gene is a sodium-dependent transporter that uptakes gamma-aminobutyric acid (GABA), an inhibitory neurotransmitter, which ends the GABA neurotransmission. Defects in this gene may result in epilepsy, behavioral problems, or intellectual problems. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Dec 2015
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"NM_014229" in other vectors (6)
SKU Description Size Price
RC216392L1 Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, GABA), member 11 (SLC6A11), Myc-DDK-tagged 10 ug
$888.00
RC216392L2 Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, GABA), member 11 (SLC6A11), mGFP tagged 10 ug
$888.00
RC216392L3 Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, GABA), member 11 (SLC6A11), Myc-DDK-tagged 10 ug
$888.00
RC216392L4 Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, GABA), member 11 (SLC6A11), mGFP tagged 10 ug
$888.00
RG216392 SLC6A11 (tGFP-tagged) - Human solute carrier family 6 (neurotransmitter transporter, GABA), member 11 (SLC6A11) 10 ug
$489.00 $788.00
SC310207 SLC6A11 (untagged)-Human solute carrier family 6 (neurotransmitter transporter, GABA), member 11 (SLC6A11) 10 ug
$589.00

Plasmid Map for RC216392

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216392
Circular map for RC216392

file-alt Citations

MaxiK channel interactome reveals its interaction with GABA transporter 3 and heat shock protein 60 in the mammalian brain
Singh, Li, Hall et al
Neuroscience (2016) 317, 76-107 DOI: 10.1016/j.neuroscience.2015.12.058
Cited by 49
AI Summary: In this study, OriGene's HSPD1 c-Myc-DDK-tagged (RC201281) and SLC6A11 c-Myc-DDK-tagged (RC216392) clones were used to express heat shock protein 60 and GABA transporter 3, respectively, facilitating the investigation of their interactions with the MaxiK channel in mammalian brain cells.
Snippet: Clones. Heat shock protein 60 kDa 1 (HSPD1 c-Myc-DDK-tagged, ORIGENE RC201281);Human solute carrier family 6 (SLC6A11 c-Myc-DDK-tagged, ORIGENE RC216392) and BK Ca (N-terminal tagged with c-Myc epitope, pcDNA3) were used. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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