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SLC8A2 (NM_015063) Human Tagged ORF Clone

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SKU
RC216354
SLC8A2 (Myc-DDK-tagged)-Human solute carrier family 8 (sodium/calcium exchanger), member 2 (SLC8A2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $843.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC8A2
Synonyms NCX2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216354 representing NM_015063
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCCCTGGCCTTGGTGGGGGTCACACTCCTCCTGGCGGCTCCCCCATGCTCCGGGGCAGCCACCC
CAACCCCCTCCCTGCCGCCTCCCCCGGCCAATGACAGCGACACCAGCACAGGGGGCTGCCAGGGGTCCTA
CCGCTGCCAGCCGGGGGTGCTGCTGCCCGTGTGGGAGCCCGACGACCCGTCGCTGGGTGACAAGGCGGCA
CGGGCAGTGGTGTACTTTGTGGCCATGGTCTACATGTTTCTGGGAGTGTCCATCATCGCCGACCGTTTCA
TGGCGGCCATCGAGGTCATCACGTCAAAAGAGAAGGAGATCACCATCACCAAGGCCAACGGTGAGACCAG
CGTGGGCACCGTTCGCATCTGGAATGAGACGGTGTCCAACCTCACGCTCATGGCCCTGGGCTCCTCCGCA
CCTGAGATCCTGCTGTCAGTCATCGAAGTCTGCGGCCACAACTTCCAGGCGGGTGAGCTGGGCCCAGGCA
CCATCGTGGGCAGCGCTGCCTTCAACATGTTTGTGGTCATCGCCGTGTGCATCTACGTCATCCCAGCCGG
CGAGAGCCGCAAGATCAAGCACCTGAGAGTCTTCTTTGTCACTGCCTCTTGGAGCATCTTCGCCTATGTC
TGGCTTTATCTCATCCTTGCTGTTTTTTCCCCCGGTGTGGTCCAGGTGTGGGAGGCGCTGCTGACCCTGG
TCTTCTTCCCGGTGTGCGTGGTATTCGCCTGGATGGCCGACAAGCGGCTGCTCTTCTACAAGTACGTGTA
CAAGCGCTACCGCACCGACCCACGCAGCGGCATCATCATAGGCGCCGAGGGCGACCCCCCGAAGAGCATC
GAGCTGGACGGCACGTTCGTGGGCGCCGAGGCCCCAGGTGAGCTGGGCGGCCTGGGCCCGGGCCCCGCCG
AGGCGCGCGAGCTGGACGCCAGCCGCCGCGAGGTCATCCAGATCCTCAAGGACCTCAAGCAGAAGCACCC
GGACAAGGATCTGGAGCAGCTGGTGGGCATCGCCAACTACTACGCGCTGCTGCACCAGCAGAAGAGCCGC
GCCTTCTACCGCATCCAGGCCACGCGGCTGATGACCGGCGCCGGGAACGTGCTGCGCAGACACGCGGCGG
ACGCCTCGCGCAGGGCGGCGCCGGCCGAGGGCGCGGGCGAGGACGAAGACGACGGCGCCAGCCGCATCTT
CTTCGAGCCTAGCCTCTACCACTGCCTGGAGAACTGCGGCTCCGTGCTGCTGTCCGTCACGTGCCAGGGC
GGCGAGGGCAACAGCACCTTCTACGTGGACTACCGCACTGAGGACGGCTCTGCCAAGGCGGGCTCCGACT
ACGAGTACAGCGAGGGCACGCTGGTGTTCAAACCAGGCGAGACGCAGAAGGAGCTGCGCATCGGCATCAT
CGACGACGACATCTTCGAGGAGGACGAGCATTTCTTCGTGCGGCTGCTGAACCTGCGCGTGGGCGACGCG
CAGGGCATGTTCGAGCCGGACGGCGGCGGGCGGCCCAAGGGGCGGCTGGTGGCGCCGCTGCTGGCCACCG
TCACCATCCTGGACGACGACCACGCAGGCATCTTCTCCTTCCAGGACCGCCTGCTGCACGTGAGCGAGTG
CATGGGCACCGTGGACGTGCGCGTCGTGCGCAGCTCGGGCGCGCGCGGCACCGTGCGCCTTCCCTACCGC
ACGGTGGACGGCACGGCGCGCGGCGGCGGCGTGCACTACGAGGACGCGTGCGGAGAGCTGGAGTTTGGCG
ACGACGAGACCATGAAAACTCTTCAGGTGAAGATAGTTGATGACGAGGAATATGAGAAAAAGGATAATTT
CTTCATTGAGCTGGGCCAGCCCCAGTGGCTTAAGCGAGGGATTTCAGCTCTGCTACTCAATCAAGGGGAT
GGGGACAGGAAGCTAACAGCCGAGGAGGAGGAGGCTCGGAGGATAGCAGAGATGGGCAAGCCAGTTCTTG
GGGAGAACTGCCGGCTGGAGGTCATCATCGAGGAGTCATATGATTTTAAGAACACGGTGGATAAACTCAT
CAAGAAAACGAACTTGGCCTTGGTAATTGGGACCCATTCATGGAGGGAGCAGTTTTTAGAGGCAATTACG
GTGAGCGCAGGGGACGAGGAGGAGGAGGAGGACGGGTCCCGGGAGGAGCGGCTGCCGTCGTGCTTTGACT
ACGTGATGCACTTCCTGACGGTGTTCTGGAAGGTGCTCTTCGCCTGTGTGCCCCCCACCGAGTACTGCCA
CGGCTGGGCCTGCTTTGGTGTCTCCATCCTGGTCATCGGCCTGCTCACCGCCCTCATTGGGGACCTCGCC
TCCCACTTCGGCTGCACCGTTGGCCTCAAGGACTCTGTCAATGCTGTTGTCTTCGTTGCCCTGGGCACCT
CCATCCCTGACACGTTCGCCAGCAAGGTGGCGGCGCTGCAGGACCAGTGCGCCGACGCGTCCATCGGCAA
CGTGACCGGCTCCAACGCGGTGAACGTGTTCCTTGGCCTGGGCGTCGCCTGGTCTGTGGCCGCCGTGTAC
TGGGCGGTGCAGGGCCGCCCCTTCGAGGTGCGCACTGGCACGCTGGCCTTCTCCGTCACGCTCTTCACCG
TCTTCGCCTTCGTGGGCATTGCCGTGCTGCTGTACCGGCGCCGGCCGCACATCGGCGGCGAGCTGGGCGG
CCCGCGCGGACCCAAGCTCGCCACCACCGCGCTCTTCCTGGGCCTCTGGCTCCTGTACATCCTCTTCGCC
AGCCTGGAGGCGTACTGCCACATCCGGGGCTTC

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216354 representing NM_015063
Red=Cloning site Green=Tags(s)

MAPLALVGVTLLLAAPPCSGAATPTPSLPPPPANDSDTSTGGCQGSYRCQPGVLLPVWEPDDPSLGDKAA
RAVVYFVAMVYMFLGVSIIADRFMAAIEVITSKEKEITITKANGETSVGTVRIWNETVSNLTLMALGSSA
PEILLSVIEVCGHNFQAGELGPGTIVGSAAFNMFVVIAVCIYVIPAGESRKIKHLRVFFVTASWSIFAYV
WLYLILAVFSPGVVQVWEALLTLVFFPVCVVFAWMADKRLLFYKYVYKRYRTDPRSGIIIGAEGDPPKSI
ELDGTFVGAEAPGELGGLGPGPAEARELDASRREVIQILKDLKQKHPDKDLEQLVGIANYYALLHQQKSR
AFYRIQATRLMTGAGNVLRRHAADASRRAAPAEGAGEDEDDGASRIFFEPSLYHCLENCGSVLLSVTCQG
GEGNSTFYVDYRTEDGSAKAGSDYEYSEGTLVFKPGETQKELRIGIIDDDIFEEDEHFFVRLLNLRVGDA
QGMFEPDGGGRPKGRLVAPLLATVTILDDDHAGIFSFQDRLLHVSECMGTVDVRVVRSSGARGTVRLPYR
TVDGTARGGGVHYEDACGELEFGDDETMKTLQVKIVDDEEYEKKDNFFIELGQPQWLKRGISALLLNQGD
GDRKLTAEEEEARRIAEMGKPVLGENCRLEVIIEESYDFKNTVDKLIKKTNLALVIGTHSWREQFLEAIT
VSAGDEEEEEDGSREERLPSCFDYVMHFLTVFWKVLFACVPPTEYCHGWACFGVSILVIGLLTALIGDLA
SHFGCTVGLKDSVNAVVFVALGTSIPDTFASKVAALQDQCADASIGNVTGSNAVNVFLGLGVAWSVAAVY
WAVQGRPFEVRTGTLAFSVTLFTVFAFVGIAVLLYRRRPHIGGELGGPRGPKLATTALFLGLWLLYILFA
SLEAYCHIRGF

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_015063
ORF Size 2763 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015063.3
RefSeq Size 4282 bp
RefSeq ORF 2766 bp
Locus ID 6543
UniProt ID Q9UPR5
Cytogenetics 19q13.32
Protein Families Transmembrane
Protein Pathways Calcium signaling pathway
MW 100.2 kDa
Summary Mediates the electrogenic exchange of Ca(2+) against Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to cellular Ca(2+) homeostasis in excitable cells. Contributes to the rapid decrease of cytoplasmic Ca(2+) levels back to baseline after neuronal activation, and thereby contributes to modulate synaptic plasticity, learning and memory. Plays a role in regulating urinary Ca(2+) and Na(+) excretion.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_015063" in other vectors (4)
SKU Description Size Price
RC216354L3 Lenti ORF clone of Human solute carrier family 8 (sodium/calcium exchanger), member 2 (SLC8A2), Myc-DDK-tagged 10 ug
$1,143.00
RC216354L4 Lenti ORF clone of Human solute carrier family 8 (sodium/calcium exchanger), member 2 (SLC8A2), mGFP tagged 10 ug
$1,143.00
RG216354 SLC8A2 (tGFP-tagged) - Human solute carrier family 8 (sodium/calcium exchanger), member 2 (SLC8A2) 10 ug
$1,043.00
SC304218 SLC8A2 (untagged)-Human solute carrier family 8 (sodium/calcium exchanger), member 2 (SLC8A2) 10 ug
$928.00

Plasmid Map for RC216354

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216354
Circular map for RC216354

file-alt Citations

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