MAP6 (NM_207577) Human Tagged ORF Clone

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SKU
RC216255
MAP6 (Myc-DDK-tagged)-Human microtubule-associated protein 6 (MAP6), transcript variant 2
  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAP6
Synonyms MAP6-N; MTAP6; N-STOP; STOP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216255 representing NM_207577
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTGGCCGTGCATCACGAGGGCCTGCTGCATCGCCCGCTTCTGGAACCAGTTGGACAAAGCGGACA
TCGCTGTGCCGCTGGTTTTCACCAAGTACTCGGAGGCCACCGAGCACCCGGGCGCCCCGCCGCAGCCACC
GCCGCCGCAGCAGCAGGCGCAGCCGGCGCTCGCGCCCCCCTCGGCGCGCGCGGTTGCCATAGAGACGCAG
CCAGCCCAGGGCGAGTTGGATGCAGTTGCCCGGGCAACGGGGCCAGCGCCTGGGCCTACCGGCGAGCGCG
AGCCGGCGGCGGGCCCCGGCCGGAGCGGGCCGGGCCCGGGCCTGGGCTCCGGCTCCACCTCCGGCCCCGC
GGACTCGGTGATGCGGCAGGATTACCGAGCCTGGAAGGTGCAGCGGCCCGAGCCCAGCTGCCGGCCGCGC
AGCGAATACCAGCCCTCCGACGCTCCCTTCGAGCGCGAGACCCAGTACCAGAAGGACTTCCGCGCCTGGC
CGCTGCCGCGCCGCGGGGACCACCCGTGGATCCCCAAGCCCGTGCAGATCTCTGCGGCCTCCCAGGCGTC
GGCGCCCATTCTCGGGGCGCCCAAGCGCCGGCCGCAGAGCCAGGAGCGCTGGCCAGTGCAGGCCGCCGCT
GAGGCCCGGGAGCAGGAGGCGGCCCCCGGCGGAGCGGGTGGCCTGGCGGCCGGAAAGGCGTCCGGGGCGG
ACGAGCGCGACACGCGCAGGAAGGCCGGGCCTGCCTGGATTGTGCGCCGCGCCGAGGGCCTGGGGCACGA
GCAGACGCCGCTGCCCGCGGCCCAGGCCCAGGTCCAGGCCACCGGCCCCGAGGCTGGCAGGGGGCGCGCC
GCGGCGGACGCCCTCAACCGGCAAATCCGCGAGGAGGTGGCGAGTGCAGTGAGCAGCTCCTACAGGAATG
AATTCAGGGCATGGACGGACATCAAGCCTGTGAAACCAATAAAGGCCAAGCCCCAGTACAAGCCCCCAGA
TGATAAGATGGTTCATGAGACCAGCTACAGTGCTCAGTTCAAAGGAGAGGCCAGCAAGCCAACAACAGCT
GACAATAAGGTCATTGATCGCAGAAGAATACGCAGCCTCTACAGCGAACCCTTCAAGGAACCCCCAAAGG
TGGAAAAACCTAGTGTTCAGAGTTCCAAACCAAAAAAGACCTCAGCGAGCCATAAGCCCACGAGGAAGGC
CAAAGACAAGCAGGCGGTGTCAGGCCAGGCTGCCAAGAAAAAGAGCGCGGAGGGCCCGAGTACCACCAAG
CCAGACGACAAGGAGCAAAGCAAAGAGATGAACAATAAACTGGCTGAGGCGAAAGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC216255 representing NM_207577
Red=Cloning site Green=Tags(s)

MAWPCITRACCIARFWNQLDKADIAVPLVFTKYSEATEHPGAPPQPPPPQQQAQPALAPPSARAVAIETQ
PAQGELDAVARATGPAPGPTGEREPAAGPGRSGPGPGLGSGSTSGPADSVMRQDYRAWKVQRPEPSCRPR
SEYQPSDAPFERETQYQKDFRAWPLPRRGDHPWIPKPVQISAASQASAPILGAPKRRPQSQERWPVQAAA
EAREQEAAPGGAGGLAAGKASGADERDTRRKAGPAWIVRRAEGLGHEQTPLPAAQAQVQATGPEAGRGRA
AADALNRQIREEVASAVSSSYRNEFRAWTDIKPVKPIKAKPQYKPPDDKMVHETSYSAQFKGEASKPTTA
DNKVIDRRRIRSLYSEPFKEPPKVEKPSVQSSKPKKTSASHKPTRKAKDKQAVSGQAAKKKSAEGPSTTK
PDDKEQSKEMNNKLAEAKE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_207577
ORF Size 1317 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207577.1, NP_997460.1
RefSeq Size 4338 bp
RefSeq ORF 1320 bp
Locus ID 4135
UniProt ID Q96JE9
Cytogenetics 11q13.5
MW 47.2 kDa
Summary This gene encodes a microtubule-associated protein. The encoded protein is a calmodulin-binding and calmodulin-regulated protein that is involved in microtubule stabilization. Two transcript variants encoding distinct isoforms have been identified for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_207577" in other vectors (4)
SKU Description Size Price
RC216255L3 Lenti-ORF clone of MAP6 (Myc-DDK-tagged)-Human microtubule-associated protein 6 (MAP6), transcript variant 2 10 ug
$757.00
RC216255L4 Lenti-ORF clone of MAP6 (mGFP-tagged)-Human microtubule-associated protein 6 (MAP6), transcript variant 2 10 ug
$757.00
RG216255 MAP6 (tGFP-tagged) - Human microtubule-associated protein 6 (MAP6), transcript variant 2 10 ug
$657.00
SC308609 MAP6 (untagged)-Human microtubule-associated protein 6 (MAP6), transcript variant 2 10 ug
$503.00

Plasmid Map for RC216255

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Cloning scheme for RC216255

Circular map for RC216255

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