OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

RNF145 (NM_144726) Human Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
RC216033
RNF145 (Myc-DDK-tagged)-Human ring finger protein 145 (RNF145), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $633.00
4 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "RNF145 or NM_144726"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RNF145
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216033 representing NM_144726
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGAGAAACCACAGAATTGCTAGTAGTTTATGTGGAGATCAGGTCTTCTCCAAGAAAAAAAAAAAGA
AAAAAAAAAACAACATGGCTGCAAAGGAGAAACTGGAGGCAGTGTTAAATGTGGCCCTGAGGGTGCCAAG
CATCATGCTGTTGGATGTCCTGTACAGATGGGATGTCAGCTCCTTTTTCCAGCAGATCCAAAGAAGTAGC
CTTAGTAATAACCCTCTTTTCCAGTATAAGTATTTGGCTCTTAATATGCATTATGTAGGTTATATCTTAA
GTGTGGTGCTGCTAACATTGCCCAGGCAGCATCTGGTTCAGCTTTATCTATATTTTTTGACTGCTCTGCT
CCTCTATGCTGGACATCAAATTTCCAGGGACTATGTTCGGAGTGAACTGGAGTTTGCCTATGAGGGACCA
ATGTATTTAGAACCTCTCTCTATGAATCGGTTTACCACAGCCTTAATAGGTCAGTTGGTGGTGTGTACTT
TATGCTCCTGTGTCATGAAAACAAAGCAGATTTGGCTGTTTTCAGCTCACATGCTTCCTCTGCTAGCACG
ACTCTGCCTTGTTCCTTTGGAGACAATTGTTATCATCAATAAATTTGCTATGATTTTTACTGGATTGGAA
GTTCTCTATTTTCTTGGGTCTAATCTTTTGGTACCTTATAACCTTGCTAAATCTGCATACAGAGAATTGG
TTCAGGTAGTGGAGGTATATGGCCTTCTCGCCTTGGGAATGTCCCTGTGGAATCAACTGGTAGTCCCTGT
TCTTTTCATGGTTTTCTGGCTCGTCTTATTTGCTCTTCAGATTTACTCCTATTTCAGTACTCGAGATCAG
CCTGCATCACGTGAGAGGCTTCTTTTCCTTTTTCTGACAAGTATTGCGGAATGCTGCAGCACTCCTTACT
CTCTTTTGGGTTTGGTCTTCACGGTTTCTTTTGTTGCCTTGGGTGTTCTCACACTCTGCAAGTTTTACTT
GCAGGGTTATCGAGCTTTCATGAATGATCCTGCCATGAATCGGGGCATGACAGAAGGAGTAACGCTGTTA
ATCCTGGCAGTGCAGACTGGGCTGATAGAACTGCAGGTTGTTCATCGGGCATTCTTGCTCAGTATTATCC
TTTTCATTGTCGTAGCTTCTATCCTACAGTCTATGTTAGAAATTGCAGATCCTATTGTTTTGGCACTGGG
AGCATCTAGAGACAAGAGCTTGTGGAAACACTTCCGTGCTGTAAGCCTTTGTTTATTTTTATTGGTATTC
CCTGCTTATATGGCTTATATGATTTGCCAGTTTTTCCACATGGATTTTTGGCTTCTTATCATTATTTCCA
GCAGCATTCTTACCTCTCTTCAGGTTCTGGGAACACTTTTTATTTATGTCTTATTTATGGTTGAGGAATT
CAGAAAAGAGCCAGTGGAAAACATGGATGATGTCATCTACTATGTGAATGGCACTTACCGCCTGCTGGAG
TTTCTTGTGGCCCTCTGTGTGGTGGCCTATGGCGTCTCAGAGACCATCTTTGGAGAATGGACAGTGATGG
GCTCAATGATCATCTTCATTCATTCCTACTATAACGTGTGGCTTCGGGCCCAGCTGGGGTGGAAGAGCTT
TCTTCTCCGCAGGGATGCTGTGAATAAGATTAAATCGTTACCCATTGCTACGAAAGAGCAGCTTGAGAAA
CACAATGATATTTGTGCCATCTGTTATCAGGACATGAAATCTGCTGTGATCACGCCTTGCAGTCATTTTT
TCCATGCAGGCTGTCTTAAGAAATGGCTGTATGTCCAGGAGACCTGCCCTCTGTGCCACTGCCATCTGAA
AAACTCCTCCCAGCTTCCAGGATTAGGAACTGAGCCAGTTCTACAGCCTCATGCTGGAGCTGAGCAAAAC
GTCATGTTTCAGGAAGGTACTGAACCCCCAGGCCAGGAGCATACTCCAGGGACCAGGATACAGGAAGGTT
CCAGGGACAATAATGAGTACATTGCCAGACGACCAGATAACCAGGAAGGGGCTTTTGACCCCAAAGAATA
TCCTCACAGTGCGAAAGATGAAGCACATCCTGTTGAATCAGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216033 representing NM_144726
Red=Cloning site Green=Tags(s)

MMRNHRIASSLCGDQVFSKKKKKKKKNNMAAKEKLEAVLNVALRVPSIMLLDVLYRWDVSSFFQQIQRSS
LSNNPLFQYKYLALNMHYVGYILSVVLLTLPRQHLVQLYLYFLTALLLYAGHQISRDYVRSELEFAYEGP
MYLEPLSMNRFTTALIGQLVVCTLCSCVMKTKQIWLFSAHMLPLLARLCLVPLETIVIINKFAMIFTGLE
VLYFLGSNLLVPYNLAKSAYRELVQVVEVYGLLALGMSLWNQLVVPVLFMVFWLVLFALQIYSYFSTRDQ
PASRERLLFLFLTSIAECCSTPYSLLGLVFTVSFVALGVLTLCKFYLQGYRAFMNDPAMNRGMTEGVTLL
ILAVQTGLIELQVVHRAFLLSIILFIVVASILQSMLEIADPIVLALGASRDKSLWKHFRAVSLCLFLLVF
PAYMAYMICQFFHMDFWLLIIISSSILTSLQVLGTLFIYVLFMVEEFRKEPVENMDDVIYYVNGTYRLLE
FLVALCVVAYGVSETIFGEWTVMGSMIIFIHSYYNVWLRAQLGWKSFLLRRDAVNKIKSLPIATKEQLEK
HNDICAICYQDMKSAVITPCSHFFHAGCLKKWLYVQETCPLCHCHLKNSSQLPGLGTEPVLQPHAGAEQN
VMFQEGTEPPGQEHTPGTRIQEGSRDNNEYIARRPDNQEGAFDPKEYPHSAKDEAHPVESA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144726
ORF Size 2073 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144726.2
RefSeq Size 3362 bp
RefSeq ORF 2076 bp
Locus ID 153830
UniProt ID Q96MT1
Cytogenetics 5q33.3
Domains RING
Protein Families Druggable Genome, Transmembrane
MW 78.7 kDa
Summary E3 ubiquitin ligase that catalyzes the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. In response to bacterial infection, negatively regulates the phagocyte oxidative burst by controlling the turnover of the NADPH oxidase complex subunits. Promotes monoubiquitination of CYBA and 'Lys-48'-linked polyubiquitination and degradation of CYBB NADPH oxidase catalytic subunits, both essential for the generation of antimicrobial reactive oxygen species. Involved in the maintenance of cholesterol homeostasis. In response to high sterol concentrations ubiquitinates HMGCR, a rate-limiting enzyme in cholesterol biosynthesis, and targets it for degradation. The interaction with INSIG1 is required for this function. In addition, triggers ubiquitination of SCAP, likely inhibiting its transport to the Golgi apparatus and the subsequent processing/maturation of SREBPF2, ultimately downregulating cholesterol biosynthesis.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_144726" in other vectors (4)
SKU Description Size Price
RC216033L3 Lenti-ORF clone of RNF145 (Myc-DDK-tagged)-Human ring finger protein 145 (RNF145), transcript variant 2 10 ug
$933.00
RC216033L4 Lenti-ORF clone of RNF145 (mGFP-tagged)-Human ring finger protein 145 (RNF145), transcript variant 2 10 ug
$933.00
RG216033 RNF145 (tGFP-tagged) - Human ring finger protein 145 (RNF145), transcript variant 2 10 ug
$833.00
SC306192 RNF145 (untagged)-Human ring finger protein 145 (RNF145), transcript variant 2 10 ug
$697.00

Plasmid Map for RC216033

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216033
Circular map for RC216033

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products