KAT5 (NM_182710) Human Tagged ORF Clone

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SKU
RC215979
KAT5 (Myc-DDK-tagged)-Human K(lysine) acetyltransferase 5 (KAT5), transcript variant 1
  $508.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KAT5
Synonyms cPLA2; ESA1; HTATIP; HTATIP1; NEDFASB; PLIP; TIP; TIP60; ZC2HC5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215979 representing NM_182710
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGGTGGTGAGTCCGGTGCCCGGGGCGGGGCGGAGGGAGCCAGGGGAGGTGGGTAGAGCCCGAG
GCCCCCCAGTAGCCGACCCTGGCGTCGCGCTGTCTCCCCAGGGGGAGATAATCGAGGGCTGCCGCCTACC
CGTGCTGCGGCGGAACCAGGACAACGAAGATGAGTGGCCCCTGGCCGAGATCCTGAGCGTGAAGGACATC
AGTGGCCGGAAGCTTTTCTACGTCCATTACATTGACTTCAACAAACGTCTGGATGAATGGGTGACGCATG
AGCGGCTGGACCTAAAGAAGATCCAGTTCCCCAAGAAAGAGGCCAAGACCCCCACTAAGAACGGACTTCC
TGGGTCCCGTCCTGGCTCTCCAGAGAGAGAGGTGCCGGCCTCGGCGCAGGCCAGCGGGAAGACCTTGCCA
ATCCCGGTCCAGATCACACTCCGCTTCAACCTGCCCAAGGAGCGGGAGGCCATTCCCGGTGGCGAGCCTG
ACCAGCCGCTCTCCTCCAGCTCCTGCCTGCAGCCCAACCACCGCTCAACGAAACGGAAGGTGGAGGTGGT
TTCACCAGCAACTCCAGTGCCCAGCGAGACAGCCCCGGCCTCGGTTTTTCCCCAGAATGGAGCCGCCCGT
AGGGCAGTGGCAGCCCAGCCAGGACGGAAGCGAAAATCGAATTGTTTGGGCACTGATGAGGACTCCCAGG
ACAGCTCTGATGGAATACCGTCAGCACCACGCATGACTGGCAGCCTGGTGTCTGATCGAAGCCACGACGA
CATCGTCACCCGGATGAAGAACATTGAGTGCATTGAGCTGGGCCGGCACCGCCTCAAGCCGTGGTACTTC
TCCCCGTACCCACAGGAACTCACCACATTGCCTGTCCTCTACCTGTGCGAGTTCTGCCTCAAGTACGGCC
GTAGTCTCAAGTGTCTTCAGCGTCATTTGACCAAGTGTGACCTACGACATCCTCCAGGCAATGAGATTTA
CCGCAAGGGCACCATCTCCTTCTTTGAGATTGATGGACGTAAGAACAAGAGTTATTCCCAGAACCTGTGT
CTTTTGGCCAAGTGTTTCCTTGACCATAAGACACTGTACTATGACACAGACCCTTTCCTCTTCTACGTCA
TGACAGAGTATGACTGTAAGGGCTTCCACATCGTGGGCTACTTCTCCAAGGAGAAAGAATCAACGGAAGA
CTACAATGTGGCCTGCATCCTAACCCTGCCTCCCTACCAGCGCCGGGGCTACGGCAAGCTGCTGATCGAG
TTCAGCTATGAACTCTCCAAAGTGGAAGGGAAAACAGGGACCCCTGAGAAGCCCCTCTCAGACCTTGGCC
TCCTATCCTATCGAAGCTACTGGTCCCAGACCATCCTGGAGATCCTGATGGGGCTGAAGTCGGAGAGCGG
GGAGAGGCCACAGATCACCATCAATGAGATTAGTGAAATCACCAGCATCAAGAAGGAGGATGTCATCTCC
ACTCTGCAGTACCTCAATCTCATCAACTACTACAAGGGCCAGTACATCCTCACACTGTCAGAGGACATCG
TGGATGGCCATGAGCGGGCCATGCTCAAGCGGCTCCTGCGGATCGACTCCAAGTGTCTGCACTTCACTCC
CAAGGACTGGAGCAAGAGGGGGAAGTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC215979 representing NM_182710
Red=Cloning site Green=Tags(s)

MAEVVSPVPGAGRREPGEVGRARGPPVADPGVALSPQGEIIEGCRLPVLRRNQDNEDEWPLAEILSVKDI
SGRKLFYVHYIDFNKRLDEWVTHERLDLKKIQFPKKEAKTPTKNGLPGSRPGSPEREVPASAQASGKTLP
IPVQITLRFNLPKEREAIPGGEPDQPLSSSSCLQPNHRSTKRKVEVVSPATPVPSETAPASVFPQNGAAR
RAVAAQPGRKRKSNCLGTDEDSQDSSDGIPSAPRMTGSLVSDRSHDDIVTRMKNIECIELGRHRLKPWYF
SPYPQELTTLPVLYLCEFCLKYGRSLKCLQRHLTKCDLRHPPGNEIYRKGTISFFEIDGRKNKSYSQNLC
LLAKCFLDHKTLYYDTDPFLFYVMTEYDCKGFHIVGYFSKEKESTEDYNVACILTLPPYQRRGYGKLLIE
FSYELSKVEGKTGTPEKPLSDLGLLSYRSYWSQTILEILMGLKSESGERPQITINEISEITSIKKEDVIS
TLQYLNLINYYKGQYILTLSEDIVDGHERAMLKRLLRIDSKCLHFTPKDWSKRGKW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182710
ORF Size 1638 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182710.3
RefSeq Size 2335 bp
RefSeq ORF 1641 bp
Locus ID 10524
UniProt ID Q92993
Cytogenetics 11q13.1
Protein Families Druggable Genome, Transcription Factors
MW 61.6 kDa
Summary The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_182710" in other vectors (6)
SKU Description Size Price
RC215979L1 Lenti ORF clone of Human K(lysine) acetyltransferase 5 (KAT5), transcript variant 1, Myc-DDK-tagged 10 ug
$808.00
RC215979L2 Lenti ORF clone of Human K(lysine) acetyltransferase 5 (KAT5), transcript variant 1, mGFP tagged 10 ug
$808.00
RC215979L3 Lenti ORF clone of Human K(lysine) acetyltransferase 5 (KAT5), transcript variant 1, Myc-DDK-tagged 10 ug
$808.00
RC215979L4 Lenti ORF clone of Human K(lysine) acetyltransferase 5 (KAT5), transcript variant 1, mGFP tagged 10 ug
$808.00
RG215979 KAT5 (tGFP-tagged) - Human K(lysine) acetyltransferase 5 (KAT5), transcript variant 1 10 ug
$489.00 $708.00
SC307492 KAT5 (untagged)-Human K(lysine) acetyltransferase 5 (KAT5), transcript variant 1 10 ug
$509.00

Plasmid Map for RC215979

Download sequence for

Cloning scheme for RC215979

Circular map for RC215979

file-alt Citations

Epigenetic Regulation of MicroRNA Expression by Hepatitis B Virus Pre-S2 Mutant Promotes Hepatocellular Carcinoma Tumorigenesis
Lin, Jeng, Shih et al
Liver Int (2026) 46 (4), e70582 DOI: 10.1111/liv.70582
Cited by 1
AI Summary: OriGene products, specifically the KAT3B (RC223265) and KAT5 (RC215979) cDNA plasmids and the pCMV6-Entry vector (PS100001), were used in this study to investigate the epigenetic mechanisms by which the hepatitis B virus pre-S2 mutant regulates microRNA expression in hepatocellular carcinoma.
Snippet: Agilent Technologies, Santa Clara, CA, USA) as described (Figure S1) [37]. The lysine acetyltransferase 3B (KAT3B;RC223265) and KAT5 (RC215979) cDNA plasmids and their background vector pCMV6-Entry (PS100001) were purchased from OriGene (Rockville, MD, USA). The oligonucleotides of all miRNA mimics and their NC mimic used in this study were
Elevation of androgen receptor promotes prostate cancer metastasis by induction of epithelial-mesenchymal transition and reduction of KAT5
Lin, Jan, Kuo et al
Cancer Sci. (2018) 109 (11), 3564-3574 DOI: 10.1111/cas.13776
AI Summary: OriGene’s TissueScan Prostate Tissue qPCR Array (HPRT101~103) was used to measure KAT5 mRNA levels across different stages of prostate cancer, helping to elucidate the relationship between KAT5 expression and prostate cancer progression in this study.
Snippet: The KAT5 mRNA level of human prostate tissue and different stages of prostate cancer was determined on TissueScan Prostate Tissue qPCR Array HPRT101~103 (OriGene Technologies, Rockville, MD, USA) according to the manufacturer's instructions with Maxima SYBR Green/ROX qPCR Master Mix (2×) (Fermentas, Glen Burnie, MA, USA) and analyzed by Applied Biosystems 7500 Real‐Time PCR system (Thermo Scientific, Waltham, MA, USA). …
FigureFigureFigure
Showing 1-2 of 2 papers.
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