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COX7A2 (NM_001865) Human Tagged ORF Clone

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SKU
RC215973
COX7A2 (Myc-DDK-tagged)-Human cytochrome c oxidase subunit VIIa polypeptide 2 (liver) (COX7A2), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol COX7A2
Synonyms COX7AL; COX7AL1; COXVIIa-L; COXVIIAL; VIIAL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215973 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCGGAATCTGCTGGCTCTTCGTCAGATTGGGCAGAGGACGATAAGCACTGCTTCCCGCAGGCATT
TTAAAAATAAAGTTCCGGAGAAGCAAAAACTGTTCCAGGAGGATGATGAAATTCCACTGTATCTAAAGGG
TGGGGTAGCTGATGCCCTCCTGTATAGAGCCACCATGATTCTTACAGTTGGTGGAACAGCATATGCCATA
TATGAGCTGGCTGTGGCTTCATTTCCCAAGAAGCAGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215973 protein sequence
Red=Cloning site Green=Tags(s)

MLRNLLALRQIGQRTISTASRRHFKNKVPEKQKLFQEDDEIPLYLKGGVADALLYRATMILTVGGTAYAI
YELAVASFPKKQE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_001865
ORF Size 249 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001865.6
RefSeq Size 708 bp
RefSeq ORF 252 bp
Locus ID 1347
UniProt ID P14406
Cytogenetics 6q14.1
Domains COX7a
Protein Families Transmembrane
Protein Pathways Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Oxidative phosphorylation, Parkinson's disease
MW 9.4 kDa
Summary Cytochrome c oxidase, the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of three catalytic subunits encoded by mitochondrial genes, and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, while the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (liver isoform) of subunit VIIa, with this polypeptide being present in both muscle and non-muscle tissues. In addition to polypeptide 2, subunit VIIa includes polypeptide 1 (muscle isoform), which is present only in muscle tissues, and a related protein, which is present in all tissues. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4 and 14. provided by RefSeq, Oct 2009
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Documents
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"NM_001865" in other vectors (4)
SKU Description Size Price
RC215973L3 Lenti ORF clone of Human cytochrome c oxidase subunit VIIa polypeptide 2 (liver) (COX7A2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged 10 ug
$450.00
RC215973L4 Lenti ORF clone of Human cytochrome c oxidase subunit VIIa polypeptide 2 (liver) (COX7A2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged 10 ug
$450.00
RG215973 COX7A2 (tGFP-tagged) - Human cytochrome c oxidase subunit VIIa polypeptide 2 (liver) (COX7A2), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$489.00
SC118990 COX7A2 (untagged)-Human cytochrome c oxidase subunit VIIa polypeptide 2 (liver) (COX7A2), nuclear gene encoding mitochondrial protein, transcript variant 1 10 ug
$150.00

file-alt Citations

Two independent respiratory chains adapt OXPHOS performance to glycolytic switch
Fernández-Vizarra, López-Calcerrada, Sierra-Magro et al
Cell Metab (2022) 34 (11), 1792-1808.e6 DOI: 10.1016/j.cmet.2022.09.005
Cited by 96
AI Summary: In this study, OriGene products—including COX7A2L-Myc-DDK (Cat# RC202697), COX7A1-Myc-DDK (Cat# RC201154), COX7A2-Myc-DDK (Cat# RC215973), and pCMV6-A-Entry-Hygro (Cat# PS100024)—were used to investigate the roles of COX7A subunit isoforms in organizing mitochondrial respiratory chain structures and adapting oxidative phosphorylation to metabolic changes.
Snippet: pSp-CAS9(BB)-2A-GFP (PX458) Ran et al., 2013 Addgene# 48138 COX7A2L- Myc-DDK Origene Cat#RC202697 COX7A1- Myc-DDK Origene Cat#RC201154 COX7A2- Myc-DDK Origene Cat#RC215973 pCMV6-A-Entry-Hygro Origene Cat# PS100024 COX7A2L-Myc-DDK in pCMV6-A-Entry-Hygro Lobo-Jarne et al., 2018 N/A
Showing 1-1 of 1 papers.
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