UPF2 (NM_080599) Human Tagged ORF Clone

SKU RC215944

UPF2 (Myc-DDK-tagged)-Human UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: UPF2
• Synonyms: HUPF2; RENT2; smg-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215944 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCAGCTGAGCGTAAAAAGCCAGCAAGTATGGAAGAAAAAGACTCTTTACCAAACAACAAGGAAAAAG; ACTGCAGTGAAAGGCGGACAGTGAGCAGCAAGGAGAGGCCAAAAGACGATATCAAGCTCACTGCCAAGAA; GGAGGTCAGCAAGGCCCCTGAAGACAAGAAGAAGAGACTGGAAGATGATAAGAGAAAAAAGGAAGACAAG; GAACGCAAGAAAAAAGACGAAGAAAAGGTGAAGGCAGAGGAAGAATCAAAGAAAAAAGAAGAGGAAGAAA; AAAAGAAACATCAAGAGGAAGAGAGAAAGAAGCAAGAAGAGCAGGCCAAACGTCAGCAAGAAGAAGAAGC; AGCTGCTCAGATGAAAGAAAAAGAAGAATCCATTCAGCTTCATCAGGAAGCTTGGGAACGACATCATTTA; AGAAAGGAACTTCGTAGCAAAAACCAAAATGCTCCGGACAGCCGACCAGAGGAAAACTTCTTCAGCCGCC; TCGACTCAAGTTTGAAGAAAAATACTGCTTTTGTCAAGAAACTAAAAACTATTACAGAACAACAGAGAGA; CTCCTTGTCCCATGATTTTAATGGCCTAAATTTAAGCAAATACATTGCAGAAGCTGTAGCTTCCATCGTG; GAAGCAAAACTAAAAATCTCTGATGTGAACTGTGCTGTGCACCTCTGCTCTCTCTTTCACCAGCGTTATG; CTGACTTTGCCCCATCACTTCTTCAGGTCTGGAAAAAACATTTTGAAGCAAGGAAAGAGGAGAAAACACC; TAACATCACCAAGTTAAGAACTGATTTGCGTTTTATTGCAGAATTGACAATAGTTGGGATTTTCACTGAC; AAGGAAGGTCTTTCCTTAATCTATGAACAGCTAAAAAATATTATTAATGCTGATCGGGAGTCCCACACTC; ATGTCTCTGTAGTGATTAGTTTCTGTCGACATTGTGGAGATGATATTGCTGGACTTGTACCAAGGAAAGT; AAAGAGTGCTGCAGAGAAGTTTAATTTGAGTTTTCCTCCTAGTGAGATAATTAGTCCAGAGAAACAACAG; CCCTTCCAGAATCTTTTAAAAGAGTACTTTACGTCTTTGACCAAACACCTGAAAAGGGACCACAGGGAGC; TCCAGAATACTGAGAGACAAAACAGGCGCATTCTACATTCTAAAGGGGAGCTCAGTGAAGATAGACATAA; ACAGTATGAGGAATTTGCTATGTCTTACCAGAAGCTGCTGGCAAATTCTCAATCCTTAGCAGACCTTTTG; GATGAAAATATGCCAGATCTTCCTCAAGACAAACCAACACCAGAAGAACATGGGCCTGGAATTGATATAT; TCACACCTGGTAAACCTGGAGAATATGACTTGGAAGGTGGTATATGGGAAGATGAAGATGCTCGGAATTT; TTATGAGAACCTCATTGATTTGAAGGCTTTTGTCCCAGCCATCTTGTTTAAAGACAATGAAAAAAGTTGT; CAGAATAAAGAGTCCAACAAAGATGATACCAAAGAGGCAAAAGAATCTAAGGAGAATAAGGAGGTATCAA; GTCCCGATGATTTGGAACTTGAGTTGGAGAATCTAGAAATTAATGATGACACCTTAGAATTAGAGGGTGG; AGATGAAGCTGAAGATCTTACAAAGAAACTTCTTGATGAACAAGAACAAGAAGATGAGGAAGCCAGCACT; GGATCTCATCTCAAGCTCATAGTAGATGCTTTCCTACAGCAGTTACCCAACTGTGTCAACCGAGATCTGA; TAGACAAGGCAGCAATGGATTTTTGCATGAACATGAACACAAAAGCAAACAGGAAGAAGTTGGTACGGGC; ACTCTTCATAGTTCCTAGACAAAGGTTGGATTTGCTACCATTTTATGCAAGATTGGTTGCTACATTGCAT; CCCTGCATGTCTGATGTAGCAGAGGATCTTTGTTCCATGCTGAGGGGGGATTTCAGATTTCATGTACGGA; AAAAGGACCAGATCAATATTGAAACAAAGAATAAAACTGTTCGTTTTATAGGAGAACTAACTAAGTTTAA; GATGTTCACCAAAAATGACACACTGCATTGTTTAAAGATGCTTCTGTCAGACTTCTCTCATCACCATATT; GAAATGGCATGCACCCTGCTGGAGACATGTGGACGGTTTCTTTTCAGATCTCCAGAATCTCACCTGAGGA; CCAGTGTACTTTTGGAGCAAATGATGAGAAAGAAGCAAGCAATGCATCTTGATGCGAGATACGTCACAAT; GGTAGAGAATGCATATTACTACTGCAACCCACCTCCAGCTGAAAAAACCGTGAAAAAGAAACGTCCTCCT; CTCCAGGAATATGTCCGGAAACTTTTGTACAAGGATCTCTCTAAGGTTACCACCGAGAAGGTTTTGAGAC; AGATGCGAAAGCTGCCCTGGCAGGACCAAGAAGTGAAAGACTATGTTATTTGTTGTATGATAAACATCTG; GAATGTGAAATATAATAGTATTCATTGTGTAGCCAACCTCTTAGCAGGACTAGTGCTCTACCAAGAGGAT; GTTGGGATCCACGTTGTGGATGGAGTGTTAGAAGATATTCGATTAGGAATGGAGGTTAATCAACCTAAAT; TTAATCAGAGGCGCATCAGCAGTGCCAAGTTCTTAGGAGAACTTTACAATTACCGAATGGTGGAATCAGC; TGTTATTTTCAGAACTCTGTATTCTTTTACCTCATTTGGTGTTAATCCTGATGGCTCTCCAAGTTCCCTG; GACCCACCTGAGCATCTTTTCAGAATTAGACTCGTATGCACTATTCTGGACACATGTGGCCAGTACTTTG; ACAGAGGTTCCAGTAAACGAAAACTTGATTGTTTCCTTGTATATTTTCAGCGTTATGTTTGGTGGAAGAA; AAGTTTGGAGGTTTGGACAAAAGACCATCCATTTCCTATTGATATAGATTACATGATCAGTGATACACTA; GAACTGCTAAGACCAAAGATCAAACTCTGTAATTCTCTGGAAGAATCCATCAGGCAGGTACAAGACTTGG; AACGAGAATTCTTAATAAAACTAGGCCTAGTAAATGACAAAGACTCAAAAGATTCTATGACAGAAGGAGA; AAATCTTGAAGAGGATGAAGAAGAAGAAGAAGGTGGGGCTGAAACAGAAGAACAATCTGGAAATGAAAGT; GAAGTAAATGAGCCAGAAGAAGAGGAGGGTTCTGATAATGATGATGATGAGGGAGAAGAAGAGGAGGAAG; AGAATACAGATTACCTTACAGATTCCAATAAGGAAAATGAAACCGATGAAGAGAATACTGAGGTAATGAT; TAAAGGCGGTGGACTTAAGCATGTACCTTGTGTAGAAGATGAGGACTTCATTCAAGCTCTGGATAAAATG; ATGCTAGAAAATCTACAGCAACGAAGTGGTGAATCTGTTAAAGTGCACCAACTAGATGTTGCCATTCCTT; TGCATCTCAAAAGCCAGCTGAGGAAAGGGCCCCCACTGGGAGGTGGGGAAGGAGAGGCTGAGTCTGCAGA; CACAATGCCGTTTGTCATGTTAACAAGAAAAGGCAATAAACAGCAGTTTAAGATCCTTAATGTACCCATG; TCCTCTCAACTTGCTGCAAATCACTGGAACCAGCAACAGGCAGAACAAGAAGAGAGGATGAGAATGAAAA; AGCTCACACTAGATATCAATGAACGGCAAGAACAAGAAGATTATCAAGAAATGTTGCAGTCTCTTGCACA; GCGCCCAGCTCCAGCAAACACCAATCGTGAGAGGCGGCCTCGCTACCAACATCCGAAGGGAGCACCTAAT; GCAGATCTAATCTTTAAGACTGGTGGGAGGAGACGT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215944 protein sequence; Red=Cloning site Green=Tags(s); ; MPAERKKPASMEEKDSLPNNKEKDCSERRTVSSKERPKDDIKLTAKKEVSKAPEDKKKRLEDDKRKKEDK; ERKKKDEEKVKAEEESKKKEEEEKKKHQEEERKKQEEQAKRQQEEEAAAQMKEKEESIQLHQEAWERHHL; RKELRSKNQNAPDSRPEENFFSRLDSSLKKNTAFVKKLKTITEQQRDSLSHDFNGLNLSKYIAEAVASIV; EAKLKISDVNCAVHLCSLFHQRYADFAPSLLQVWKKHFEARKEEKTPNITKLRTDLRFIAELTIVGIFTD; KEGLSLIYEQLKNIINADRESHTHVSVVISFCRHCGDDIAGLVPRKVKSAAEKFNLSFPPSEIISPEKQQ; PFQNLLKEYFTSLTKHLKRDHRELQNTERQNRRILHSKGELSEDRHKQYEEFAMSYQKLLANSQSLADLL; DENMPDLPQDKPTPEEHGPGIDIFTPGKPGEYDLEGGIWEDEDARNFYENLIDLKAFVPAILFKDNEKSC; QNKESNKDDTKEAKESKENKEVSSPDDLELELENLEINDDTLELEGGDEAEDLTKKLLDEQEQEDEEAST; GSHLKLIVDAFLQQLPNCVNRDLIDKAAMDFCMNMNTKANRKKLVRALFIVPRQRLDLLPFYARLVATLH; PCMSDVAEDLCSMLRGDFRFHVRKKDQINIETKNKTVRFIGELTKFKMFTKNDTLHCLKMLLSDFSHHHI; EMACTLLETCGRFLFRSPESHLRTSVLLEQMMRKKQAMHLDARYVTMVENAYYYCNPPPAEKTVKKKRPP; LQEYVRKLLYKDLSKVTTEKVLRQMRKLPWQDQEVKDYVICCMINIWNVKYNSIHCVANLLAGLVLYQED; VGIHVVDGVLEDIRLGMEVNQPKFNQRRISSAKFLGELYNYRMVESAVIFRTLYSFTSFGVNPDGSPSSL; DPPEHLFRIRLVCTILDTCGQYFDRGSSKRKLDCFLVYFQRYVWWKKSLEVWTKDHPFPIDIDYMISDTL; ELLRPKIKLCNSLEESIRQVQDLEREFLIKLGLVNDKDSKDSMTEGENLEEDEEEEEGGAETEEQSGNES; EVNEPEEEEGSDNDDDEGEEEEEENTDYLTDSNKENETDEENTEVMIKGGGLKHVPCVEDEDFIQALDKM; MLENLQQRSGESVKVHQLDVAIPLHLKSQLRKGPPLGGGEGEAESADTMPFVMLTRKGNKQQFKILNVPM; SSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPN; ADLIFKTGGRRR; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080599
• ORF Size: 3816 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_080599.2, NP_542166.1
• RefSeq Size: 5387 bp
• RefSeq ORF: 3819 bp
• Locus ID: 26019
• UniProt ID: Q9HAU5
• Cytogenetics: 10p14
• MW: 147.8 kDa
• Summary: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein. [provided by RefSeq, Jul 2008]